Animal and plant responses for steers grazing switchgrass and big bluestem pastures

Native warm-season grasses can provide large amounts of high-quality forage during the midsummer months. Maximum potential benefits depend on management of the entire cool-and warm-season grazing system. This study compared two grazing systems for the warm-season pasture component. Fall-born steers grazed pastures of \u27Cave-in-Rock\u27 switchgrass or \u27Roundtree\u27 big bluestem over three years using either continuous or rotational grazing systems. Pasture carrying time for switchgrass and bluestem pastures and steer weight gain were considerably higher for the rotational grazing program than for the continuous grazing regimen

Vegetative Filter Strip Removal of Metals in Runoff from Poultry Litter-Amended Fescuegrass Plots

Runoff from land areas amended with poultry (Gallus gallus domesticus) manure can contain elevated concentrations of metals such as Cu, Fe, and Zn. Vegetative filter strips (VFS) can reduce runoff concentrations of animal manure components, but reported studies have typically focused on nutrients and solids rather than metals. This experiment assessed the impact of VFS length (0 to 12 m) on concentrations and mass losses of Cu, Fe, K, Na, Ni, and Zn in runoff from fescuegrass (Festuca arundinacea Schreb.) plots (1.5 m wide × 6 and 12 m long) treated with poultry litter. The runoff was produced from simulated rainfall applied at 50 mm h-1 until 1 h of runoff had occurred. Runoff Ni concentrations were below detection levels in all cases. Concentrations of Cu, Fe, K, Na, and Zn did not differ between litter-treated plot lengths but were significantly (p \u3c 0.001) affected by VFS length, decreasing in an approximately firstorder fashion. Means separation indicated that concentrations of Cu, Fe, K, and Zn did not significantly decrease after a VFS length of 3 m, while Na concentrations decreased up to a VFS length of 6 m. Mass transport of only Cu significantly decreased with increasing VFS, suggesting that VFS removal mechanisms such as adsorption to clay particles might play a larger role with regard to Cu than to Fe, K, Na, and Zn

Indicator Bacteria Concentrations of Two Northwest Arkansas Streams in Relation to Flow and Season

Concentrations of indicator bacteria such as fecal coliform (FC) and fecal streptococcus (FS) are often used to assess the suitability of waters for their intended use(s) and to allocate resources for water quality improvement measures. There is evidence, however, that concentrations of FC and FS can be influenced by variables such as season and flow rate during sampling, which could lead to biased results. The objective of this study was to assess the impacts of season and flow rate on concentrations of FC and FS. Fecal indicator bacteria concentrations were measured for approximately three years at five sites on two Northwest Arkansas streams. Flow data were collected at two of the five sites. Land use in the basins draining the streams was primarily pasture (57-90%) and forest (6-40%). Significant seasonal influences on FC and FS concentrations were detected for all sampling sites, with the highest concentrations occurring in summer. On the two sites with flow data, flow rate generally had a significant effect on FC and FS concentrations during all seasons, with FC and FS concentrations increasing with flow rate. Ratios of FC and FS, which have been used in the past to differentiate between animal and human sources of fecal pollution, did not appear to reliably indicate the major sources of fecal indicator bacteria. The findings of this study suggest a potential for fixed sampling intervals to contribute to biased results. The issue of biased results might be avoided by sampling during times of year and flow conditions that support the intended use(s) of the waters

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival