Optimization of a pulsed-field gel electrophoresis for molecular typing of Proteus mirabilis

Objective: For the detection of outbreaks caused byProteus mirabilis, strains clonal relations are determinedmethods as “pulsed-field gel electrophoresis (PFGE)”.The aim of this study was optimization of a pulsed-fieldgel electrophoresis for molecular typing of P. mirabilis.Methods: In this study, PFGE’ protocol is optimized foruse in molecular typing of P. mirabilis. Phylogenetic analyzesof strains were evaluated with Bionumerics softwaresystem (version 6.01; Applied Maths, Sint-Martens-Latem, Belgium).Results: This protocol compared with Gram-negativebacteria PFGE protocols, NotI enzyme is suitable for thisbacterium. Electrophoresis conditions should be revealedas; - block 1: initial pulse duration 1 sec, ending pulseduration 30 sec, striking angle 120°, the current 6 V/cm2,temperature 14°C, time 8 hours; - block 2: initial pulseduration 30 sec, ending pulse duration 70 sec, strikingangle 120°, the current 6 V/cm2, temperature 14°C, time16 hours; - TBE, pH=8.4.Conclusion: P. mirabilis strains were typed by PFGE andBionumerics analysis program were determined clonal relationships.The procedure was simple, reproducible andsuitable for these bacteria. Also it was evaluated, becauseof reducing time, the solution volumes and enzymes canbe economically. Outbreaks of nosocomial infections dueto bacteria studied assessment and the potential to provideuseful information about the degree of prevalence.This optimized protocol is allowed different centers’ PFGEresults to compare with other laboratories results. J ClinExp Invest 2013; 4 (3): 306-312Key words: Proteus mirabilis, molecular typing, pulsedfieldgel electrophoresis

Magnetic resonance dacryocystography: Its role in the diagnosis and treatment plan of lacrimal drainage system obstructions

Objective: To evaluate the role of magnetic resonance dacryocystography (MR-DCG) technique in patients with obstruction of lacrimal drainage system. Methods: A total of 40 patients who had presented to the ophthalmology clinic were suspected to have obstruction of lacrimal drainage system, were evaluated with MR-DCG after instillation of 0.5% Gd-DTPA conjunctival contrast medium. Dacryocystorhinostomy (DCR) was performed in patients who were found to have lacrimal drainage system obstruction on either side on MR-DCG. Results: Obstruction of lacrimal drainage system was successfully detected in a total of 49 eyes of 40 patients undergoing examination with MR-DCG. The MR-DCG findings of 29 nasolacrimal systems were compared with the intraoperative findings in 28 out of 40 patients who had undergone the DCR operation. The sensitivity of MR-DCG was determined as 100% and specificity as 96.7% for identification of nasolacrimal system obstruction when compared with the intraoperative findings, and MR-DCG was found to detect obstruction with high accuracy. Conclusion: MR-DCG has a high success rate in detection of lacrimal drainage system obstructions and the level and cause of the obstruction

Effect of Sport Training and Education on Q Angle in Young Males and Females

The purpose of this study investigation of the effect of sport training and education on Q angle in young males and females. The Quadriceps Q angles of 240 female and 600 male subject were measured in both supine and standing positions by goniometer. It was used t-tests for statistical analyses. In this study, the average age was found to be 19.16 for women and 21.120 for men. The vast majority of participants are in the 17-22 age range. In this study, was found the mean Q angle of female and male subjects at supine position 15.710 and 13.020. On the other hand Q angle of female and male subjects at standing position were 17.050 and 14.040 respectively. The difference between mean Q angle of female and male subject was statistically significant (p0.05). Finally, the mean dominant knee Q angle of young females and males seemed to be in normal range. The high value of the knee Q angle of the dominant leg should be considered to be greater than 17 for males and 20 for females. The effect of physical activity on Q-angle should be investigated more extensively. It is thought that sport education has a decreasing effect on Q angle

INTERVAL VERSUS CONTINUOUS SMALL-SIDED SOCCER GAMES WITH SAME PITCH SIZE AND NUMBER OF PLAYERS

The aim of the study compared effects of intermittent and continuous small-sided games (SSGs) on physiological and technical activities in soccer players. Totally 16 male amateur soccer players (Mean age: 22.37±1.69 years, height: 170.89±5.96 cm and body mass: 67.37±7.47 kg) were recruited as participants. Subjects were separated in 4 groups of 4 players according to the Yo-Yo test results and their coaches' comments. Four-a-side SSGs were used in a pitch size of 26 m x 34 m and 2 different small-sided games were used in this study. Intermittent SSG (ISSG) included a series of 3 bouts of 6 min duration with 3 min recovery between sets. Continuous SSG (CSSG) was a bout of 18 min duration with no recovery. Heart rate (HR), the rating of perceived exertion (RPE), and blood lactate concentration (LA) were measured as physical parameters and shoot on target, total pass, accurate pass, inaccurate pass, interception, dribbling, tackle, and possessing the ball were counted by Mathball Match Analyses System. A repeated measures ANOVA method, paired and unpaired tests were used to analyses data. The results of this study suggest that SSGs might cause increases of HR, RPE, and LA and that intermittent and continuous SSGs might not bring about an alteration on HR, RPE and LA

An Experimental Study of NOMA for Connected Autonomous Vehicles

Connected autonomous vehicles (CAV) constitute an important application of future-oriented traffic management .A vehicular system dominated by fully autonomous vehicles requires a robust and efficient vehicle-to-everything (V2X) infrastructure that will provide sturdy connection of vehicles in both short and long distances for a large number of devices, requiring high spectral efficiency (SE). Power domain non-orthogonal multiple access (PD-NOMA) technique has the potential to provide the required high SE levels. In this paper, a vehicular PD-NOMA testbed is implemented using software defined radio (SDR) nodes. The main concerns and their corresponding solutions arising from the implementation are highlighted. The bit error rates(BER) of vehicles with different channel conditions are measured for mobile and stationary cases. The extent of the estimation errors on the success rate beyond the idealized theoretical analysis view is investigated and the approaches to alleviate these errors are discussed. Finally, our perspective on possible PD-NOMA based CAV deployment scenarios is presented in terms of performance constraints and expectancy along with the overlooked open issues.Comment: 7 Pages, 6 figure

Anatomic Origin and Molecular Genetics in Neuroblastoma

Neuroblastoma is considered as the most common extracranial solid tumor occurring during childhood, but takes place rarely after the age of 10 years. The tumors are considered as embryonal tumors that result from the fetal or early postnatal life development and are formed from neural crest-derived cells, and their origination is from the early nerve cells which are called as neuroblasts of sympathetic nervous system. Being heterogeneous in their biological, genetic, and morphological characteristics, tumors which are distinct from other solid tumors due to their biological heterogeneity result in the clinical pattern changes from spontaneous regression to a highly aggressive metastatic disease. Neuroblastoma tumorigenesis is regulated by Myc oncogene, leading to aggressive tumor subset. Many epigenetic factors play crucial role in the disease induction and development, while regulatory effect and outcome result in epigenetic patterns distinguishing neuroectoderm, neural crest, and more mature neural states. Neuroblastoma patients’ clinical management is based on prognostic categories subtracted from studies correlating outcome and clinico-biological variables. Neuroblastoma anatomic boundaries include primarily autonomic nervous system besides other rare locations. Neuroblastoma molecular pathogenesis classifies the tumor according to the different clinical behaviors that are important for the improvement of the patients outcome and overall survival according to the different therapy modalities applied

Magnetic Resonance Imaging Texture Analysis in the Detection of Metastatic Lymph Nodes in Patients with Nasopharyngeal Carcinoma

Abstract Aims: To investigate the role of magnetic resonance imaging (MRI) texture analysis (TA) in the detection of metastatic lymph nodes in patients with nasopharyngeal carcinoma (NPC). Material and methods: Between January 2020 and October 2021, 15 NPC patients with 32 metastatic lymph nodes and 30 healthy subjects with benign lymph nodes were included in the study. The texture features compared between metastatic and benign lymph nodes. The independent predictor parameters of metastatic lymph nodes were determined using multivariate regression analysis. Receiver operator characteristics (ROC) analysis was used to evaluate the diagnostic performance of the regression models. Results: The first order texture features did not differ significantly between groups (p>0.05). Except for correlation in metastatic lymph nodes, all gray-level co-occurrence matrix (GLCM) and gray-level run length matrix (GLRLM) features were significantly different (p<0.05). The GLCM features of joint entropy, joint energy, and maximum probability; and the GLRLM features of gray level non uniformity and low gray level run emphasis were independent predictors of metastatic lymph nodes. The area under the curve (AUC) values for the GLCM regression model and GLRLM regression model were 0.975 and 0.928, respectively. Conclusion: MRI texture analysis may be useful to detect metastatic lymph nodes in patients with NPC by providing quantitative information on tissue heterogeneity and cellular composition

FARKLI TUTKALLARIN KAYIN ve SARIÇAM AĞAÇLARINDAKİ YAPIŞMA DİRENÇLERİNİN BELİRLENMESİ

Bu çalışmada, poliüretan esaslı Pu-Mon ve Pu-Ma, polivinilasetat esaslı PVAc-MA22, PVAc-MA35 ve PVAc-MA50 ile yapıştırılmış Doğu kayını (Fagus orientalis L.) ve sarıçam (Pinus Sylvestris L.) odunlarının yapışma direnci, TS EN 205 esaslarına göre belirlenmiştir. Deney sonuçlarına göre, en yüksek yapışma direnci Pu-Ma (16.01 N/mm2) ve PVAc-MA35 (15.12 N/mm2) ile yapıştırılmış kayın numunelerinde, en düşük yapışma direnci ise Pu-Mon (7.98 N/mm2) ve PVAc-MA35 (7.67 N/mm2) ile yapıştırılmış sarıçam numunelerinde tespit edilmiştir.