Changes in erythropoietin levels during space flight or space flight simulation

Two hundred and seventy samples from 24 subjects involved in 3 bedrest studies and from 3 subjects involved in Spacelab Mission Development Test 3 were assayed for erythropoietin (Ep), in an in vitro fetal mouse liver cell assay, and for ferritin using a commercially available immunoradiometric assay kit. No trends or significant changes in serum Ep were observed. Serum ferritin concentrations tended to increases slightly during the 'missions', reflecting a redirection of iron from the suppressed erythron into iron stores

Dynamic techniques for studies of secular variations in position from ranging to satellites

Satellite laser range measurements were applied to the study of latitude variation arising from polar motion, and the solid-earth and ocean tidal distortion of the earth's gravity field. Experiments involving two laser tracking stations were conducted. The relative location of one station with respect to the other was determined by performing simultaneous range measurements to a satellite from two stations several hundred kilometers apart. The application of this technique to the San Andreas Fault Experiment in California is discussed. Future capabilities of spacecraft equipped with laser retroreflectors include: (1) determination of the product of the earth's mass and gravitational constant; (2) measurement of crustal and tectonic motions; (3) determination of the elastic response of the solid-earth tidal forces; (4) measurement of the amplitudes and phase of certain components of the ocean tides; and (5) self-monitoring of the latitude and height variations of the tracking station

Evaluating the Applicability of the Fokker-Planck Equation in Polymer Translocation: A Brownian Dynamics Study

Brownian dynamics (BD) simulations are used to study the translocation dynamics of a coarse-grained polymer through a cylindrical nanopore. We consider the case of short polymers, with a polymer length, N, in the range N=21-61. The rate of translocation is controlled by a tunable friction coefficient, gamma_{0p}, for monomers inside the nanopore. In the case of unforced translocation, the mean translocation time scales with polymer length N as ~ (N-N_p)^alpha, where N_p is the average number of monomers in the nanopore. The exponent approaches the value alpha=2 when the pore friction is sufficiently high, in accord with the prediction for the case of the quasi-static regime where pore friction dominates. In the case of forced translocation, the polymer chain is stretched and compressed on the cis and trans sides, respectively, for low gamma_{0p}. However, the chain approaches conformational quasi-equilibrium for sufficiently large gamma_{0p}. In this limit the observed scaling of with driving force and chain length supports the FP prediction that is proportional to N/f_d for sufficiently strong driving force. Monte Carlo simulations are used to calculate translocation free energy functions for the system. The free energies are used with the Fokker-Planck equation to calculate translocation time distributions. At sufficiently high gamma_{0p}, the predicted distributions are in excellent agreement with those calculated from the BD simulations. Thus, the FP equation provides a valid description of translocation dynamics for sufficiently high pore friction for the range of polymer lengths considered here. Increasing N will require a corresponding increase in pore friction to maintain the validity of the FP approach. Outside the regime of low N and high pore friction, the polymer is out of equilibrium, and the FP approach is not valid.Comment: 13 pages, 11 figure

Vacuum-UV negative photoion spectroscopy of CH3F, CH3Cl and CH3Br

Using tunable vacuum-UV radiation from a synchrotron, negative ions are detected by quadrupolar mass spectrometry following photoexcitation of three gaseous halogenated methanes CH$_3$X (X = F,Cl,Br). The anions X$^-$, H$^-$, CX$^-$, CHX$^-$ and CH$_2$X$^-$ are observed, and their ion yields recorded in the range 8-35 eV. The anions show a linear dependence of signal with pressure, showing that they arise from unimolecular ion-pair dissociation, generically described as AB + h$v$ $\rightarrow$ A$^-$ + B$^+$ (+ neutrals). Absolute cross sections for ion-pair formation are obtained by calibrating the signal intensities with those of F$^-$ from both SF$_6$ and CF$_4$. The cross sections for formation of X$^-$ + CH$_3$$^+$ are much greater than for formation of CH$_2$X$^-$ + H$^+$. In common with many quadrupoles, the spectra of $m$/$z$ 1 (H$^-$) anions show contributions from all anions, and only for CH$_3$Br is it possible to perform the necessary subtraction to obtain the true H$^-$ spectrum. The anion cross sections are normalised to vacuum-UV absorption cross sections to obtain quantum yields for their production. The appearance energies of X$^-$ and CH$_2$X$^-$ are used to calculate upper limits to 298 K bond dissociation energies for D$^o$ (H$_3$C-X) and D$^o$ (XH$_2$C-H) which are consistent with literature values. The spectra suggest that most of the anions are formed indirectly by crossing of Rydberg states of the parent molecule onto an ion-pair continuum. The one exception is the lowest-energy peak of F$^-$ from CH$_3$F at 13.4 eV, where its width and lack of structure suggest it may correspond to a direct ion-pair transition

Anomalous dynamics of cell migration

Cell movement, for example during embryogenesis or tumor metastasis, is a complex dynamical process resulting from an intricate interplay of multiple components of the cellular migration machinery. At first sight, the paths of migrating cells resemble those of thermally driven Brownian particles. However, cell migration is an active biological process putting a characterization in terms of normal Brownian motion into question. By analyzing the trajectories of wildtype and mutated epithelial (MDCK-F) cells we show experimentally that anomalous dynamics characterizes cell migration. A superdiffusive increase of the mean squared displacement, non-Gaussian spatial probability distributions, and power-law decays of the velocity autocorrelations are the basis for this interpretation. Almost all results can be explained with a fractional Klein- Kramers equation allowing the quantitative classification of cell migration by a few parameters. Thereby it discloses the influence and relative importance of individual components of the cellular migration apparatus to the behavior of the cell as a whole.Comment: 20 pages, 3 figures, 1 tabl

Spectral geometry, homogeneous spaces, and differential forms with finite Fourier series

Let G be a compact Lie group acting transitively on Riemannian manifolds M and N. Let p be a G equivariant Riemannian submersion from M to N. We show that a smooth differential form on N has finite Fourier series if and only if the pull back has finite Fourier series on

Studying the X-ray hysteresis in GX 339-4: the disc and iron line over one decade

We report on a comprehensive and consistent investigation into the X-ray emission from GX 339-4. All public observations in the 11 year RXTE archive were analysed. Three different types of model - single powerlaw, broken powerlaw and a disc + powerlaw - were fitted to investigate the evolution of the disc, along with a fixed gaussian component at 6.4 keV to investigate any iron line in the spectrum. We show that the relative variation in flux and X-ray colour between the two best sampled outbursts are very similar. The decay of the disc temperature during the outburst is clearly seen in the soft state. The expected decay is S_Disc \propto T^4; we measure T^4.75\pm0.23. This implies that the inner disc radius is approximately constant in the soft state. We also show a significant anti-correlation between the iron line significant width and the X-ray flux in the soft state while in the hard state the EW is independent of the flux. This results in hysteresis in the relation between X-ray flux and both line flux and EW. To compare the X-ray binary outburst to the behaviour seen in AGN, we construct a Disc Fraction Luminosity Diagram for GX 339-4, the first for an X-ray binary. The shape qualitatively matches that produced for AGN. Linking this with the radio emission from GX 339-4 the change in radio spectrum between the disc and power-law dominated states is clearly visible.Comment: Accepted for publication in MNRAS, 20 pages, 17 figures. For high-res version see http://www.astro.soton.ac.uk/~r.j.dunn/publications.htm