Heuristic Refinement Method for the Derivation of Protein Solution Structures: Validation on Cytochrome B562

A method is described for determining the family of protein structures compatible with solution data obtained primarily from nuclear magnetic resonance (NMR) spectroscopy. Starting with all possible conformations, the method systematically excludes conformations until the remaining structures are only those compatible with the data. The apparent computational intractability of this approach is reduced by assembling the protein in pieces, by considering the protein at several levels of abstraction, by utilizing constraint satisfaction methods to consider only a few atoms at a time, and by utilizing artificial intelligence methods of heuristic control to decide which actions will exclude the most conformations. Example results are presented for simulated NMR data from the known crystal structure of cytochrome b562 (103 residues). For 10 sample backbones an average root-mean-square deviation from the crystal of 4.1 A was found for all alpha-carbon atoms and 2.8 A for helix alpha-carbons alone. The 10 backbones define the family of all structures compatible with the data and provide nearly correct starting structures for adjustment by any of the current structure determination methods

Quantum Dynamics of the Slow Rollover Transition in the Linear Delta Expansion

We apply the linear delta expansion to the quantum mechanical version of the slow rollover transition which is an important feature of inflationary models of the early universe. The method, which goes beyond the Gaussian approximation, gives results which stay close to the exact solution for longer than previous methods. It provides a promising basis for extension to a full field theoretic treatment.Comment: 12 pages, including 4 figure

The Calibration of the HST Kuiper Belt Object Search: Setting the Record Straight

The limiting magnitude of the HST data set used by Cochran et al. (1995) to detect small objects in the Kuiper belt is reevaluated, and the methods used are described in detail. It is shown, by implanting artificial objects in the original HST images, and re-reducing the images using our original algorithm, that the limiting magnitude of our images (as defined by the 50% detectability limit) is $V=28.4$. This value is statistically the same as the value found in the original analysis. We find that $\sim50$ of the moving Kuiper belt objects with $V=27.9$ are detected when trailing losses are included. In the same data in which these faint objects are detected, we find that the number of false detections brighter than $V=28.8$ is less than one per WFPC2 image. We show that, primarily due to a zero-point calibration error, but partly due to inadequacies in modeling the HST'S data noise characteristics and Cochran et al.'s reduction techniques, Brown et al. 1997 underestimate the SNR of objects in the HST dataset by over a factor of 2, and their conclusions are therefore invalid.Comment: Accepted to ApJ Letters; 10 pages plus 3 figures, LaTe

A Damping of the de Haas-van Alphen Oscillations in the superconducting state

Deploying a recently developed semiclassical theory of quasiparticles in the superconducting state we study the de Haas-van Alphen effect. We find that the oscillations have the same frequency as in the normal state but their amplitude is reduced. We find an analytic formulae for this damping which is due to tunnelling between semiclassical quasiparticle orbits comprising both particle-like and hole-like segments. The quantitative predictions of the theory are consistent with the available data.Comment: 7 pages, 5 figure

Unquenched QCD with Light Quarks

We present recent results in unquenched lattice QCD with two degenerate light sea quarks using the truncated determinant approximation (TDA). In the TDA the infrared modes contributing to the quark determinant are computed exactly up to some cutoff in quark off-shellness (typically 2$\Lambda_{QCD}$). This approach allows simulations to be performed at much lighter quark masses than possible with conventional hybrid MonteCarlo techniques. Results for the static energy and topological charge distributions are presented using a large ensemble generated on very coarse (6$^4$) but physically large lattices. Preliminary results are also reported for the static energy and meson spectrum on 10$^3$x20 lattices (lattice scale $a^{-1}$=1.15 GeV) at quark masses corresponding to pions of mass $\leq$ 200 MeV. Using multiboson simulation to compute the ultraviolet part of the quark determinant the TDA approach becomes an exact with essentially no increase in computational effort. Some preliminary results using this fully unquenched algorithm are presented.Comment: LateX, 39 pages, 16 eps figures, 1 ps figur

Reservation status and priorities for Tasmanian plants I. Angiospermae (Dicotyledonae)

Almost one-fifth of Tasmanian native dicotyledonous angiosperms are not known from any national park or equivalent reserve. Extinct, endangered, vulnerable and unreserved species are "most common among annuals and least common among woody plants. The unreserved species have their distributions concentrated between Launceston and Hobart in the dry, naturally grassy Midlands. A minimum reservation strategy is suggested for those species for which this option still exists

The Fading Radio Emission from SN 1961V: Evidence for a Type II Peculiar Supernova?

Using the Very Large Array (VLA), we have detected radio emission from the site of SN 1961V in the Sc galaxy NGC 1058. With a peak flux density of 0.063 +/- 0.008 mJy/beam at 6 cm and 0.147 +/- 0.026 mJy/beam at 18 cm, the source is non-thermal, with a spectral index of -0.79 +/- 0.23. Within errors, this spectral index is the same value reported for previous VLA observations taken in 1984 and 1986. The radio emission at both wavelengths has decayed since the mid 1980's observations with power-law indices of beta(20cm) = -0.69 +/- 0.23 and beta(6cm) = -1.75 +/- 0.16. We discuss the radio properties of this source and compare them with those of Type II radio supernovae and luminous blue variables.Comment: 19 pages, 3 figures; To appear in the Astronomical Journa

Heavy-light baryonic mass splittings from the lattice

We present lattice estimates of the mass of the heavy-light baryons $\Lambda_b$ and $\Xi_b$ obtained using propagating heavy quarks. For $\Lambda_b$ our result is $M_{\Lambda_b}=5.728 \pm 0.144 \pm 0.018$ GeV, after extrapolation to the continuum limit and in the quenched approximation.Comment: 3 pages postscript, Contribution to Lattice'9

Sensitivity Improvement of a 1-μm Ladar System Incorporating an Active Optical Fiber Preamplifier

In an effort to increase the SNR of a continuous wave, 1-μm all solid state ladar system, a rare-earth-doped optical fiber amplifier is investigated as a preamplifier for ladar return signals. The experimental system is detailed and a theoretical analysis of the fiber amplifier\u27s effect on both heterodyne and direct detection schemes is provided. Beginning with the optical powers incident on the detector, the signal and noises are analyzed, through the detector electronics, to predict the SNR. The SNR is then plotted as a function of the return signal power, and a SNR threshold is defined to determine a minimum detectable signal power. The return signals required to attain the SNR threshold are then compared for four cases: direct detection with and without the fiber amplifier and heterodyne detection with and without the fiber amplifier. For the direct detection scheme considered, our results predict a sensitivity increase of 20.6 dB with the addition of the fiber amplifier, yet for heterodyne detection the predicted sensitivity increase is only 3.1 dB

Comparative study of selected indoor concentration from selective laser sintering process using virgin and recycled polyamide nylon (pa12)

Additive manufacturing (AM) stands out as one of the promising technologies that have huge potential towards manufacturing industry. The study on additive manufacturing impact on the environment and occupational exposure are attracting growing attention recently. However, most of the researcher focus on desktop and fused deposition modelling type and less attention given to the industrial type of AM. Usually, during the selective laser sintering process, recycle powder will be used again to reduce cost and waste. This article compares the PM 2.5, carbon dioxide (CO2) and total volatile organic compound (TVOC) concentration between virgin and recycles powder using polyamide-nylon (PA12) towards indoor concentration. Four phases of sampling involve during air sampling accordingly to the Industry Code of Practice on Indoor Air Quality 2010 by DOSH Malaysia. It was found that PM 2.5 and CO2 concentration are mainly generated during the pre-printing process. The recycle powder tended to appear higher compared to virgin powder in terms of PM 2.5, and CO2. The peak value of PM 2.5 is 1452 μg/m3 and CO2 is 1218 ppm are obtained during the pre-printing process during 8 hours of sampling. TVOC concentration from recycling powder is slightly higher during the post- printing phase where confirm the influence of the powder cake and PA12 temperature from the printing process. In summary, this work proves that elective laser sintering (SLS) machine operators are exposed to a significant amount of exposure during the SLS printing process. Mitigation strategies and personal protective equipment are suggested to reduce occupational exposure