Reduction of trimethylamine N-oxide to trimethylamine by the human gut microbiota: supporting evidence for ‘metabolic retroversion’

Dietary sources of methylamines such as choline, trimethylamine (TMA), trimethylamine N-oxide (TMAO), phosphatidylcholine (PC) and carnitine are present in a number of foodstuffs, including meat, fish, nuts and eggs. It is recognized that the gut microbiota is able to convert choline to TMA in a fermentation-like process. Similarly, PC and carnitine are converted to TMA by the gut microbiota. It has been suggested that TMAO is subject to ‘metabolic retroversion’ in the gut (i.e. it is reduced to TMA by the gut microbiota, with this TMA being oxidized to produce TMAO in the liver). Sixty-six strains of human faecal and caecal bacteria were screened on solid and liquid media for their ability to utilize trimethylamine N-oxide (TMAO), with metabolites in spent media profiled by Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy. Enterobacteriaceae produced mostly TMA from TMAO, with caecal/small intestinal isolates of Escherichia coli producing more TMA than their faecal counterparts. Lactic acid bacteria (enterococci, streptococci, bifidobacteria) produced increased amounts of lactate when grown in the presence of TMAO, but did not produce large amounts of TMA from TMAO. The presence of TMAO in media increased the growth rate of Enterobacteriaceae; while it did not affect the growth rate of lactic acid bacteria, TMAO increased the biomass of these bacteria. The positive influence of TMAO on Enterobacteriaceae was confirmed in anaerobic, stirred, pH-controlled batch culture fermentation systems inoculated with human faeces, where this was the only bacterial population whose growth was significantly stimulated by the presence of TMAO in the medium. We hypothesize that dietary TMAO is used as an alternative electron acceptor by the gut microbiota in the small intestine/proximal colon, and contributes to microbial population dynamics upon its utilization and retroversion to TMA, prior to absorption and secondary conversion to TMAO by hepatic flavin-containing monooxygenases. Our findings support the idea that oral TMAO supplementation is a physiologically-stable microbiota-mediated strategy to deliver TMA at the gut barrier

Observation of correlations up to the micrometer scale in sliding charge-density waves

High-resolution coherent x-ray diffraction experiment has been performed on the charge density wave (CDW) system K$_{0.3}$MoO$_3$. The $2k_F$ satellite reflection associated with the CDW has been measured with respect to external dc currents. In the sliding regime, the $2k_F$ satellite reflection displays secondary satellites along the chain axis which corresponds to correlations up to the micrometer scale. This super long range order is 1500 times larger than the CDW period itself. This new type of electronic correlation seems inherent to the collective dynamics of electrons in charge density wave systems. Several scenarios are discussed.Comment: 4 pages, 3 figures Typos added, references remove

Flexible Wedge Phased Array Transducers for Inspecting Variable-Geometry or Complex Components

The transmission of ultrasound from the transducer into the inspected component is a determining factor in the performance of ultrasound inspections. Various coupling solutions exist to ensure this transmission. The most frequently used are:• Immersion of the component in water tank: This coupling presents the best acoustic performance (low attenuation, coupling homogeneity, no intermediate interface). However, the inspected parts need to be fully immersed and thus complex control systems are required.• Coupling by direct contact with a liquid couplant, or via a rigid wedge or a delay line with liquid couplant at the interfaces: This coupling requires simpler control systems for the inspection, but the homogeneity of the couplant film and attenuation in the wedges deteriorate the signal. The geometry of the inspected part can make the coupling more difficult to setup, particularly if the surface is complex or varies from one point to another. The problem becomes critical when the dimensions of the transducer are large in comparison with the local curvature of the interface. The use of transducers that are flexible, or that are fitted with a flexible wedge, improves the quality of the coupling for components with complex or variable geometry, and in some cases, makes it possible to do certain inspections that currently have no solution. This article presents the recent developments and results obtained in the context of transducers with flexible wedges, in particular: • Design options; • Flexible membranes and mechanical interfaces development for PA transducers; • Mechanical supports development for manual or automated use; • Acoustic performance, and wear resistance tests; These studies have demonstrated the contribution of flexible wedge transducers to various applications, with acoustic performances similar to that of immersion and easy implementation comparable to standard contact inspections, while remaining compatible with an industrial use. The detailed results will be presented, as well as the possibilities for the future developments of transducers with flexible wedges

Improving the Acoustic Performance of Linear Multi-Element Transducers

The electro-acoustic performance of transducers has a direct impact on the performance of ultrasound inspections. The signal/noise ratio and the resolution (both axial and lateral) are key factors for detecting and/or proportioning the indications being sought. The signal/noise ratio partly depends on the sensitivity and the signal/noise ratio of the transducer itself. The axial resolution depends on the length of the signal and therefore, for a given maximum frequency, on the damping of the transducer. Sensitivity and damping are often considered antagonistic, as damping traditionally reduces resonance and therefore sensitivity. Earlier studies have demonstrated the advantages gained through using piezocomposite technology to improve this compromise. These two parameters also depend on the acoustic adaptation to the coupling medium (water, plexiglass, rexolite, steel, etc.), and according to the design used, performance deteriorates more or less as one moves further from the nominal use. In addition to sensitivity and the signal/noise ratio, other parameters such as the angular acceptance and resistance to abrasion are sometimes to be integrated in the expected performances. This article presents the recent developments undertaken and tested in the context of improving the acoustic performance of multi-element probes: - Identification of the components that influence performance; - Simulations; - Selection of the configurations that meet the needs of various applications; - The experimental results obtained; - Comparison with the simulations. These studies have led to the development of a design expertise for responding to requests for custom-made, industrial, multi-element probes with improved performance, for production runs from a single item to dozens, even hundreds. The detailed results will be presented, as well as the possibilities for future development

Quantitative Decoding of Interactions in Tunable Nanomagnet Arrays Using First Order Reversal Curves

To develop a full understanding of interactions in nanomagnet arrays is a persistent challenge, critically impacting their technological acceptance. This paper reports the experimental, numerical and analytical investigation of interactions in arrays of Co nanoellipses using the first-order reversal curve (FORC) technique. A mean-field analysis has revealed the physical mechanisms giving rise to all of the observed features: a shift of the non-interacting FORC-ridge at the low-H$_c$ end off the local coercivity H$_c$ axis; a stretch of the FORC-ridge at the high-H$_c$ end without shifting it off the H$_c$ axis; and a formation of a tilted edge connected to the ridge at the low-H$_c$ end. Changing from flat to Gaussian coercivity distribution produces a negative feature, bends the ridge, and broadens the edge. Finally, nearest neighbor interactions segment the FORC-ridge. These results demonstrate that the FORC approach provides a comprehensive framework to qualitatively and quantitatively decode interactions in nanomagnet arrays.Comment: 19 pages, 4 figures. 9 page supplemental material including 3 figure

Reduction of trimethylamine N-oxide to trimethylamine by the human gut microbiota: supporting evidence for ‘metabolic retroversion’

Dietary sources of methylamines such as choline, trimethylamine (TMA), trimethylamine N-oxide (TMAO), phosphatidylcholine (PC) and carnitine are present in a number of foodstuffs, including meat, fish, nuts and eggs. It is recognized that the gut microbiota is able to convert choline to TMA in a fermentation-like process. Similarly, PC and carnitine are converted to TMA by the gut microbiota. It has been suggested that TMAO is subject to ‘metabolic retroversion’ in the gut (i.e. it is reduced to TMA by the gut microbiota, with this TMA being oxidized to produce TMAO in the liver). Sixty-six strains of human faecal and caecal bacteria were screened on solid and liquid media for their ability to utilize trimethylamine N-oxide (TMAO), with metabolites in spent media profiled by Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy. Enterobacteriaceae produced mostly TMA from TMAO, with caecal/small intestinal isolates of Escherichia coli producing more TMA than their faecal counterparts. Lactic acid bacteria (enterococci, streptococci, bifidobacteria) produced increased amounts of lactate when grown in the presence of TMAO, but did not produce large amounts of TMA from TMAO. The presence of TMAO in media increased the growth rate of Enterobacteriaceae; while it did not affect the growth rate of lactic acid bacteria, TMAO increased the biomass of these bacteria. The positive influence of TMAO on Enterobacteriaceae was confirmed in anaerobic, stirred, pH-controlled batch culture fermentation systems inoculated with human faeces, where this was the only bacterial population whose growth was significantly stimulated by the presence of TMAO in the medium. We hypothesize that dietary TMAO is used as an alternative electron acceptor by the gut microbiota in the small intestine/proximal colon, and contributes to microbial population dynamics upon its utilization and retroversion to TMA, prior to absorption and secondary conversion to TMAO by hepatic flavin-containing monooxygenases. Our findings support the idea that oral TMAO supplementation is a physiologically-stable microbiota-mediated strategy to deliver TMA at the gut barrier

Type II and IV radio bursts in the active period October-November 2003

In this report we present the Type II and IV radio bursts observed and analyzed by the radio spectrograph ARTEMIS IV1, in the 650-20MHz frequency range, during the active period October-November 2003. These bursts exhibit very rich fine structures such fibers, pulsations and zebra patterns which is associated with certain characteristics of the associated solar flares and CMEs.Comment: Recent Advances in Astronomy and Astrophysics: 7th International Conference of the Hellenic Astronomical Society. AIP Conference Proceedings, Volume 848, pp. 199-206 (2006

Ten Years of the Solar Radiospectrograph ARTEMIS-IV

The Solar Radiospectrograph of the University of Athens (ARTEMIS-IV) is in operation at the Thermopylae Satellite Communication Station since 1996. The observations extend from the base of the Solar Corona (650 MHz) to about 2 Solar Radii (20 MHz) with time resolution 1/10-1/100 sec. The instruments recordings, being in the form of dynamic spectra, measure radio flux as a function of height in the corona; our observations are combined with spatial data from the Nancay Radioheliograph whenever the need for 3D positional information arises. The ARTEMIS-IV contribution in the study of solar radio bursts is two fold- Firstly, in investigating new spectral characteristics since its high sampling rate facilitates the study of fine structures in radio events. On the other hand it is used in studying the association of solar bursts with interplanetary phenomena because of its extended frequency range which is, furthermore, complementary to the range of the WIND/WAVES receivers and the observations may be readily combined. This reports serves as a brief account of this operation. Joint observations with STEREO/WAVES and LOFAR low frequency receivers are envisaged in the future

Type II Shocks Characteristics: Comparison with associated CMEs and Flares

A number of metric (100-650 MHz) typeII bursts was recorded by the ARTEMIS-IV radiospectrograph in the 1998-2000 period; the sample includes both CME driven shocks and shocks originating from flare blasts. We study their characteristics in comparison with characteristics of associated CMEs and flares.Comment: Recent Advances in Astronomy and Astrophysics: 7th International Conference of the Hellenic Astronomical Society. AIP Conference Proceedings, Volume 848, pp. 238-242 (2006

Deep imaging survey of young, nearby austral stars: VLT/NACO near-infrared Lyot-coronographic observations

Context. High contrast and high angular resolution imaging is the optimal search technique for substellar companions to nearby stars at physical separations larger than typically 10 AU. Two distinct populations of substellar companions, brown dwarfs and planets, can be probed and characterized. As a result, fossile traces of processes of formation and evolution can be revealed by physical and orbital properties, both for individual systems and as an ensemble. Aims. Since November 2002, we have conducted a large, deep imaging, survey of young, nearby associations of the southern hemisphere. Our goal is detection and characterization of substellar companions with projected separations in the range 10–500 AU. We have observed a sample of 88 stars, primarily G to M dwarfs, younger than 100 Myr, and within 100 pc of Earth. Methods. The VLT/NACO adaptive optics instrument of the ESO Paranal Observatory was used to explore the faint circumstellar environment between typically 0.1 and 10". Diffraction-limited observations in H and K_s-band combined with Lyot-coronagraphy enabled us to reach primary star-companion brightness ratios as small as 10^(-6). The existence of planetary mass companions could therefore be probed. We used a standardized observing sequence to precisely measure the position and flux of all detected sources relative to their visual primary star. Repeated observations at several epochs enabled us to discriminate comoving companions from background objects. Results. We report the discovery of 17 new close (0.1–5.0") multiple systems. HIP 108195 AB and C (F1 III-M6), HIP 84642 AB (a~14 AU, K0-M5) and TWA22 AB (a~1.8 AU; M6-M6) are confirmed comoving systems. TWA22 AB is likely to be a rare astrometric calibrator that can be used to test evolutionary model predictions. Among our complete sample, a total of 65 targets were observed with deep coronagraphic imaging. About 240 faint companion candidates were detected around 36 stars. Follow-up observations with VLT or HST for 83% of these stars enabled us to identify a large fraction of background contaminants. Our latest results that pertain to the substellar companions to GSC 08047-00232, AB Pic and 2M1207 (confirmed during this survey and published earlier), are reviewed. Finally, a statistical analysis of our complete set of coronagraphic detection limits enables us to place constraints on the physical and orbital properties of giant planets between typically 20 and 150 AU