Commercial fishing, conservation and compatibility in the Northwestern Hawaiian Islands

A critical issue in marine ecosystem-based management is addressing the compatibility of extractive use with ecosystem protection. Analysis of the protected area planning process for the Northwestern Hawaiian Islands suggests the key elements of an ecosystem-based approach to protected area management include establishing a common understanding among decision makers of ecosystem boundaries and ecosystem condition, informed by the best available science. A participatory process is proposed that relies on an informed assessment of whether extractive use compromises ecological integrity and is consistent with conservation objectives. Best practices for compatibility determinations are proposed for multi-agency protected area management and planning.Compatibility Marine protected area Conservation Fishery management Ecosystem-based management Fishery Coral reef

Functional characterization of GcpE, an essential enzyme of the non-mevalonate pathway of isoprenoid biosynthesis

The gcpE gene product controls one of the terminal steps of isoprenoid biosynthesis via the mevalonate independent 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. This pathway is utilized by a variety of eubacteria, the plastids of algae and higher plants, and the plastid-like organelle of malaria parasites. Recombinant GcpE protein from the hyperthermophilic bacterium Thermus thermophilus was produced in Escherichia coli and purified under dioxygen-free conditions. The protein was enzymatically active in converting 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcPP) into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) in the presence of dithionite as reductant. The maximal specific activity was 0.6 micromol x min(-1) x mg(-1) at pH 7.5 and 55 degrees C. The kcat value was 0.4 s(-1) and the K(m) value for HMBPP 0.42 mM