Dynamics and regulation of ovarian antral follicular waves in sheep

The focus of the present thesis was on ultrasonographic, endocrine and molecular characterization of ovarian antral follicular waves in sheep. Transrectal ultrasonography and computer assisted image analysis were used to determine the feasibility of detecting ovulation and the forming corpus luteum (CL) and to non-invasively monitor CL differentiation and growth. High resolution transrectal ultrasonography and hormone measurements were used to assess changes in numbers of small ovarian antral follicles and their relationships to the emergence of follicular waves in cyclic ewes and to correlate pulsatile secretion of gonadotropins with follicular growth in a wave, during the mid to late-luteal phase of the ovine estrous cycle. A series of experiments were conducted, using treatment with injections of ovine follicle stimulating hormone (oFSH) and measurement of serum concentrations of FSH, in cyclic and anestrous sheep, to investigate the existence of follicular dominance. We also evaluated the characteristics of secretory patterns of FSH that are critical for follicular wave emergence, in anestrous ewes. The possible existence of an endogenous rhythm of FSH secretion, independent of ovarian antral follicular dynamics, was studied in ovariectomized ewes. Finally, ovarian antral follicles at defined stages of growth in a follicular wave (based on transrectal ultrasonographic observations) were collected from separate groups of sheep by ovariectomy, to profile the expression patterns of steroidogenic enzymes (3¦Â-HSD, 17¦Á-OH and aromatase) using immunohistochemistry and gray-scale densitometric analysis. The results of the present studies showed that it is possible to detect ovulation and visualize developing CL as early as 12-24 h after ovulation in the ewe. Changes in echotexture of the CL were closely associated with its morphological and functional characteristics, and we concluded that computer assisted image analysis holds promise for the noninvasive monitoring of CL differentiation and growth. Follicles reaching ovulatory diameter (¡Ý 5 mm) emerged and grew in a wave-like pattern in sheep, but without variation in the number of small follicles (1-3 mm in diameter), as seen in cattle. We concluded that all follicles that are recruited to grow beyond 2-3-mm in diameter, to 4-mm diameter in a wave, succeed in reaching an ovulatory diameter of ¡Ý 5 mm in the ewe. The emergence and growth of ovarian antral follicles in follicular waves, in sheep, do not require changes in LH secretion and may perhaps involve changes in the follicular sensitivity to LH. The largest follicle of a wave, in sheep, appears to have limited effects on other small follicles and on the time of emergence of the next follicular wave. Thus, functional dominance, as is present in cattle, may be absent in sheep. An endogenous rhythm for periodic peaks in serum FSH concentrations that is independent of ovarian follicular dynamics may exist in sheep. The expression patterns of steroidogenic enzymes, in the theca and granulosa compartments of antral follicles growing in each follicular wave in the ewe, paralleled serum estradiol concentrations, with the exception of the concentrations of 3¦Â-HSD in granulosa cells, which increased continuously from follicles 3 mm in diameter to the preovulatory follicle after the LH surge. The largest follicle of any follicular wave, irrespective of the stage of the cycle, would appear to be mature enough to ovulate if a gonadotropin surge is provided

Maternal and perinatal outcome in abruption placenta in tertiary care center: a record based case series study

Background: Abruptio placenta is premature separation of the normally implanted placenta before delivery. It is one of the leading causes of maternal morbidity and neonatal morbidity and mortality, more so because of the difficulty to predict the acute event. It occurs in approximately one in 80 deliveries and remains a significant cause of perinatal mortality and morbidity. Objective of the study was to study maternal and perinatal outcome in cases of abruption. Methods: 42 cases of pregnant women who presented with abruption placenta to HSK hospital and Research Centre, S. Nijalingappa Medical College, a tertiary care centre at Bagalkot, from January 2022 to December 2022. Maternal and perinatal characteristics were retrieved from the case papers. Results: Among 42 cases of abruption 70% delivered vaginally and 30% underwent caesarean section. 84% had preterm delivery. 66% were still born. 21% were case of severe anaemia and required blood transfusion. 1 had maternal mortality. Conclusions: The availability of advanced emergency obstetric care across greater number of referral hospitals has been responsible for decreasing the morbidity and mortality associated with many obstetric conditions. However, the challenge with abruptio placenta is the difficulty of predicting this condition, and hence appropriate management. As of now, early referral to tertiary care centres, better availability of blood and blood products and early interventions have the potential to limit adverse maternal and perinatal outcomes. Research regarding predictors of placental abruption can help in improving maternal and perinatal outcome.

Transforming growth factor-beta family members are regulated during induced luteolysis in cattle

The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis in vivo. On day 10 of the estrous cycle, cows received an injection of prostaglandin F2α (PGF) and luteal samples were obtained from separate groups of cows (n= 4-5 cows per time-point) at 0, 2, 12, 24 or 48 h after treatment. Since TGF beta family comprises more than 30 ligands, we focused in some candidates genes such as activin receptors (ACVR-1A, -1B, -2A, -2B) AMH, AMHR2, BMPs (BMP-1, -2, -3, -4, -6 and -7), BMP receptors (BMPR 1A, -1B and -2), inhibin subunits (INH-A, -BA, -BB) and betaglycan (TGFBR3). The mRNA levels of BMP4, BMP6 and INHBA were higher at 2 h after PGF administration (P<0.05) in comparison to 0 h. The relative mRNA abundance of BMP1, BMP2, BMP3, BMP4, BMP6, ACVR1B, INHBA and INHBB was upregulated up to 12 h post PGF (P<0.05). On the other hand, TGFBR3 mRNA that codes for a reservoir of ligands that bind to TGF-beta receptors, was lower at 48 h. In conclusion, findings from this study demonstrated that genes encoding several TGFβ family members are expressed in a time-specific manner after PGF administration

The Bile Acid Synthesis Pathway Is Present and Functional in the Human Ovary

Background: Bile acids, end products of the pathway for cholesterol elimination, are required for dietary lipid and fat-soluble vitamin absorption and maintain the balance between cholesterol synthesis in the liver and cholesterol excretion. They are composed of a steroid structure and are primarily made in the liver by the oxidation of cholesterol. Cholesterol is also highly abundant in the human ovarian follicle, where it is used in the formation of the sex steroids. Methodology/Principal Findings: Here we describe for the first time evidence that all aspects of the bile acid synthesis pathway are present in the human ovarian follicle, including the enzymes in both the classical and alternative pathways, the nuclear receptors known to regulate the pathway, and the end product bile acids. Furthermore, we provide functional evidence that bile acids are produced by the human follicular granulosa cells in response to cholesterol presence in the culture media. Conclusions/Significance: These findings establish a novel pathway present in the human ovarian follicle that has the capacity to compete directly with sex steroid synthesis

Clock genes and their genomic distributions in three species of salmonid fishes: Associations with genes regulating sexual maturation and cell cycling

<p>Abstract</p> <p>Background</p> <p>Clock family genes encode transcription factors that regulate clock-controlled genes and thus regulate many physiological mechanisms/processes in a circadian fashion. Clock1 duplicates and copies of Clock3 and NPAS2-like genes were partially characterized (genomic sequencing) and mapped using family-based indels/SNPs in rainbow trout (RT)(<it>Oncorhynchus mykiss</it>), Arctic charr (AC)(<it>Salvelinus alpinus</it>), and Atlantic salmon (AS)(<it>Salmo salar</it>) mapping panels.</p> <p>Results</p> <p>Clock1 duplicates mapped to linkage groups RT-8/-24, AC-16/-13 and AS-2/-18. Clock3/NPAS2-like genes mapped to RT-9/-20, AC-20/-43, and AS-5. Most of these linkage group regions containing the Clock gene duplicates were derived from the most recent 4R whole genome duplication event specific to the salmonids. These linkage groups contain quantitative trait loci (QTL) for life history and growth traits (i.e., reproduction and cell cycling). Comparative synteny analyses with other model teleost species reveal a high degree of conservation for genes in these chromosomal regions suggesting that functionally related or co-regulated genes are clustered in syntenic blocks. For example, anti-müllerian hormone (amh), regulating sexual maturation, and ornithine decarboxylase antizymes (oaz1 and oaz2), regulating cell cycling, are contained within these syntenic blocks.</p> <p>Conclusions</p> <p>Synteny analyses indicate that regions homologous to major life-history QTL regions in salmonids contain many candidate genes that are likely to influence reproduction and cell cycling. The order of these genes is highly conserved across the vertebrate species examined, and as such, these genes may make up a functional cluster of genes that are likely co-regulated. CLOCK, as a transcription factor, is found within this block and therefore has the potential to cis-regulate the processes influenced by these genes. Additionally, clock-controlled genes (CCGs) are located in other life-history QTL regions within salmonids suggesting that at least in part, trans-regulation of these QTL regions may also occur via Clock expression.</p

The transcriptional landscape of Rhizoctonia solani AG1-IA during infection of soybean as defined by RNA-seq

Rhizoctonia solani Kühn infects most plant families and can cause significant agriculturalyield losses worldwide; however, plant resistance to this disease is rare and short-lived,and therefore poorly understood, resulting in the use of chemical pesticides for its control.Understanding the functional responses of this pathogen during host infection can help elucidatethe molecular mechanisms that are necessary for successful host invasion. Using thepathosystem model soybean-R. solani anastomosis group AG1-IA, we examined the globaltranscriptional responses of R. solani during early and late infection stages of soybean byapplying an RNA-seq approach. Approximately, 148 million clean paired-end reads, representing93% of R. solani AG1-IA genes, were obtained from the sequenced libraries. Analysisof R. solani AG1-IA transcripts during soybean invasion revealed that most genes weresimilarly expressed during early and late infection stages, and only 11% and 15% of theexpressed genes were differentially expressed during early and late infection stages,respectively. [...

<b>Effect of body condition loss on</b><b> </b><b>hepatic and ovarian function of lactating dairy cows</b><i>Supplementary Table 1: Differential expressed genes (DEGs) of SEV vs MOD body condition</i><i>loss cows at week seven of calving. P ≤ 0.05 and |fold change| ≥ 1.5.</i>

Supplementary Table 1: Differential expressed genes (DEGs) of SEV vs MOD body condition loss cows at week seven of calving. P ≤ 0.05 and |fold change| ≥ 1.5.Cows were monitored for body condition score (BCS, 5-point scale) during the transition period (3 weeks before to 7 weeks after calving) and were classified as Moderate (MOD, < 0.75 units) or Severe (SEV, ≥ 0.75 units) loss groups. Liver biopsies were collected at Week 7 for trascriptome analysis (N=3 per group).</p