DW-MRI as a Biomarker to Compare Therapeutic Outcomes in Radiotherapy Regimens Incorporating Temozolomide or Gemcitabine in Glioblastoma

The effectiveness of the radiosensitizer gemcitabine (GEM) was evaluated in a mouse glioma along with the imaging biomarker diffusion-weighted magnetic resonance imaging (DW-MRI) for early detection of treatment effects. A genetically engineered murine GBM model [Ink4a-Arf−/− PtenloxP/loxP/Ntv-a RCAS/PDGF(+)/Cre(+)] was treated with gemcitabine (GEM), temozolomide (TMZ) +/− ionizing radiation (IR). Therapeutic efficacy was quantified by contrast-enhanced MRI and DW-MRI for growth rate and tumor cellularity, respectively. Mice treated with GEM, TMZ and radiation showed a significant reduction in growth rates as early as three days post-treatment initiation. Both combination treatments (GEM/IR and TMZ/IR) resulted in improved survival over single therapies. Tumor diffusion values increased prior to detectable changes in tumor volume growth rates following administration of therapies. Concomitant GEM/IR and TMZ/IR was active and well tolerated in this GBM model and similarly prolonged median survival of tumor bearing mice. DW-MRI provided early changes to radiosensitization treatment warranting evaluation of this imaging biomarker in clinical trials

Percepção de agentes comunitários de saúde sobre os riscos à saúde fonoaudiológica Perception of community health workers regarding risks for hearing and communication disorders

OBJETIVO: Investigar a percepção dos agentes comunitários sobre os aspectos relacionados à saúde fonoaudiológica da população usuária de um programa de saúde da família. MÉTODOS: Trata-se de estudo observacional transversal com aplicação de questionário a 85 agentes comunitários. Foram investigadas 20 situações hipotéticas abordando os temas fonoaudiológicos na rotina de trabalho dos agentes. As variáveis analisadas foram: idade, tempo de atuação, escolaridade, percepção dos riscos à saúde da população nas áreas de voz, motricidade orofacial, linguagem e audiologia. RESULTADOS: A média de idade dos agentes foi de 38 anos (±9,1), e o tempo de médio de atuação no programa 5,0 anos (±2,9). Observou-se que 80 profissionais (94%) possuíam pelo menos o Ensino Médio completo, e todos eram do gênero feminino. Entre as situações hipotéticas investigadas, os agentes demonstraram ter a percepção do risco e atitude de levá-lo para discussão da equipe em 49% das situações envolvendo o risco à saúde auditiva, 53% saúde vocal, 60% e 62% os riscos relacionados à motricidade orofacial e linguagem, respectivamente. Não foi encontrada relação entre o tempo de atuação e a percepção do risco à saúde fonoaudiológica. CONCLUSÃO: Os agentes comunitários mostraram ter percepção de muitas situações de risco à saúde fonoaudiológica dos usuários, especialmente no que se refere à saúde vocal e das estruturas e funções orofaciais. É necessário ao agente comunitário ir além das habilidades e competências conceituais e procedimentais no que se refere à saúde da comunicação humana, pois se almeja um profissional com habilidades atitudinais.<br>PURPOSE: To investigate the perception of community health workers about the aspects related to hearing and communication health of users of a family health program. METHODS: Cross-sectional observational study with questionnaire application to 85 community health workers. Twenty hypothetical situations were investigated, addressing issues related to Speech-Language Pathology and Audiology present in their routine. The variables analysed were: age, work experience, education, perception of health risks in the areas of voice, orofacial myology, language and audiology. RESULTS: The mean age of the agents was 38 years (±9.1), and their mean time of experience in the family health program was 5 years (±2.9). It was observed that 80 professionals (94%) had at least complete high school education, and all were female. Among the hypothetical situations investigated, the workers showed to have the perception of risk and attitude to take it to discussion with the team in 49% of the situations involving risks to hearing health, 53% risk to vocal health, 60% and 62% risks related to orofacial myology and language, respectively. There was no relationship between time of experience and the perception of risks. CONCLUSION: The community health workers have perception of many risk situations to hearing and communication health of the population, especially regarding voice and orofacial structures and functions. Community health workers need to go beyond the conceptual and procedural abilities and competencies regarding the health of human communication, because of the idea of professionals with attitudinal skills