Potential Substitutes of Antibiotics for Swine and Poultry Production

Early of the last century, it was detected that antibiotics added to the animal feeds at low doses and for a long time can improve technical performances such as average daily gain and gain-to-feed ratio. Since then, the antibiotics have been used worldwide as feed additives for many decades. At the end of the twentieth century, the consequences of the uses of antibiotics in animal feeds as growth promoters were informed. Since then, many research studies have been done to find other solutions to replace partly or fully to antibiotic as growth promoters (AGPs). Many achievements in finding alternatives to AGPs in which probiotics and direct-fed microorganism, prebiotics, organic acids and their salts, feed enzymes, bacteriophages, herbs, spices, and other plant extractives (phytogenics), mineral and essential oils are included

Detection of Japanese encephalitis virus and its specific antibody in abnormal swine litters in Vietnam

Japanese encephalitis is considered an essential disease-causing swine reproductive failure in Asian countries; however, significant knowledge gaps remain about this problem in actual cases. This study examined 55 cases of reproductive failure in sows, including one abortion and 54 full-term farrowing litters, to find the Japanese encephalitis virus (JEV) and specific antibodies against JEV. Haemagglutination test was used to detect specific antibodies against JEV from 63 samples of fetal thoracic fluids and newborn un-suckling piglet sera (54 thoracic fluid samples of dead fetuses, two thoracic fluid samples of abortion fetuses, seven sera samples of weakly newborn un-suckling piglets), viral isolation and RT- PCR technique was attempted from 60 brains of fetuses and newborn un-suckling piglets, histopathology sections of fetal brains which were positive with JEV confirmed by HI test and RT-PCR were also made. The results showed that 17.46% (11/63) of thoracic fluids and sera were positive for JEV. No JEV isolation was found from 60 brain samples of dead fetuses and piglets, but RNA of JEV were detected from 5 of them (8.33%). Our results suggest that JEV should be considered the important cause of swine reproductive failure in Viet Nam

Acidifiers as Alternatives for Antibiotics Reduction and Gut Health Improvement for Poultry and Swine

Using antibiotics of low doses as feed additives could support to improve poultry and swine performances. However, these applications have caused resistance of bacteria and antibiotic residues in foods of animal origins. Therefore, efforts were focused on solutions to replace antibiotics as growth promoters (AGPs). There are many alternatives for AGPs, in which organic acids are one of the important alternatives. The aim of this chapter is to review publications on these acids and their other forms namely as acidifiers using as feed additives including their names and forms, mode of actions, spectrum against bacteria, combinations among them, and latest updates on their effects on swine and poultry production. The scientific findings show that acidifiers can inhibit pathogenic bacteria growth, improve nutrient digestibility, enhance immunity and overall gut health, consequently increase performances of poultry and swine. Several acids and their salts in both liquid and solid forms have been studied and applied as poultry and swine feed additives; however, the efficacy levels and the mode of actions are dependent on the single acidifiers, their salts, and combinations among them. The uses of acidifiers in their salts and derivative forms and mixtures of different acidifiers seem to be more favorable

FLAVONOIDS AND TRITERPENOIDS FROM CALLISTEMON CITRINUS AND THEIR INHIBITORY EFFECT ON NO PRODUCTION IN LPS-STIMULATED RAW264.7 MACROPHAGES

Phytochemical investigation of the leaves and stems of Callistemon citrinus (Curtis) Skeels led to the isolation of 12 flavonoid and triterpenoid compounds,  including one new flavonoid, callistine A (1) and six known flavonoids 6,7- dimethyl-5,7-dihydroxy-4’-methoxy flavone (2), astragalin (3), quercetin (4), catechin (5), eucalyptin (6), and 8-demethyleucalyptin (7), along with 5 triterpenoids, 3-β-acetylmorolic acid (8), 3β-hydroxy-urs-11-en-13(28)-olide (9), betulinic acid (10), diospyrolide (11) and ursolic acid (12). Their chemical structures were determined from the spectroscopic evidences counting 1D- and 2D-NMR and HR-MS data. All the isolated compounds were examined for their anti-inflammatory activity against LPS-activated NO production in macrophage RAW264.7 cells. Among them, quercetin (4) and 3β-hydroxy-urs-11-en-13(28)-olide (9) showed potential inhibition activity in nitric oxide (NO)  production in RAW264.7 cells exposed to LPS

Biological activities of essential oil extracted from leaves of Atalantia sessiflora Guillauminin Vietnam

Introduction: The present study aimed to determine the chemical compositions and bioactivities of the essential oil of Atalantia sessifloraGuillaumin (A. sessiflora), including antibacterial, antimycotic, antitrichomonas, anti-inflammatory and antiviral effects. Methodology: The essential oil from leaves of A. sessiflora was extracted by hydrodistillation using a Clevenger apparatus. Chemical compositions of oil were identified by GC/MS. Antimicrobial and antitrichomonas activity were determined by the microdilution method; anti-inflammatory and antiviral were determined by the MTT method. Results: The average yield of oil was 0.46 ± 0.01% (v/w, dry leaves). A number of 45 constituents were identified by GC/MS. The essential oil comprised four main components. The oil showed antimicrobial activities against Gram-positive strains as Staphylococcus; Gram-negative bacteria such as Klebsiella pneumoniae and Escherichia coli; and finally four Candidaspecies. Enterococcus faecalis and Pseudomonas aeruginosawere least susceptible to the oil of A. sessiflora, as seen in their MIC and MLC values over 16% (v/v). Activity against Trichomonas vaginalis was also undertaken, showing IC50, IC90 and MLC values of 0.016, 0.03 and 0.06% (v/v) respectively, after 48 hours of incubation. The oil of A. sessiflora displayed activity against the nitric oxide generation with the IC50 of 95.94 ± 6.18 μg/mL. The oil was completely ineffective against tested viruses, ssRNA+, ssRNA-, dsRNA, and dsDNA viruses. Conclusions: This is the first yet comprehensive scientific report about the chemical compositions and pharmacological properties of the essential oil of A. sessiflora. Further studies should be done to evaluate the safety and toxicity of A. sessiflora oil

HỢP CHẤT STEROID VÀ FLAVONE TỪ THÂN RỄ THIÊN NIÊN KIỆN LÁ LỚN (Homalomena pendula)

Phytochemical investigation of the rhizomes of Homalomena pendula resulted in the isolation of one flavone and three steroids. These compounds were determined as tangeretin (1), ergosterol peroxide (2), sitoindoside I (3), and stigmasterol (4) on the basis of 1D and 2D NMR data and in comparison with the available data in the literature. Compounds 1‒3 were found for the first time from the genus Homamomena. The n-hexane and ethyl acetate extracts show NO production inhibitory activity in RAW 264.7 macrophage cells with IC50 values of 46.8 and 75.52 µg·mL–1.Hợp chất flavone, tangeretin (1), và ba hợp chất steroid: ergosterol peroxide (2), sitoindoside I (3) và stigmasterol (4) đã được phân lập từ thân rễ của cây thiên niên kiện lá lớn (Homalomena pendula). Cấu trúc hóa học của chúng được xác định dựa trên phân tích dữ liệu phổ cộng hưởng từ hạt nhân (1D và 2D NMR) và so sánh với các tài liệu đã công bố. Các hợp chất (1-3) được phân lập lần đầu tiên từ chi Homalomena. Cao chiết n-hexane và ethyl acetate của cây này có hoạt tính ức chế sản sinh NO trên đại thực bào RAW 264.7 kích thích bằng lipopolysaccharide với các giá trị IC50 là 46,80 và 75,52 µg·mL–1

Chicken novel leukocyte immunoglobulin-like receptor subfamilies B1 and B3 are transcriptional regulators of major histocompatibility complex class I genes and signaling pathways

Objective The inhibitory leukocyte immunoglobulin-like receptors (LILRBs) play an important role in innate immunity. The present study represents the first description of the cloning and structural and functional analysis of LILRB1 and LILRB3 isolated from two genetically disparate chicken lines. Methods Chicken LILRB1-3 genes were identified by bioinformatics approach. Expression studies were performed by transfection, quantitative polymerase chain reaction. Signal transduction was analyzed by western blots, immunoprecipitation and flow cytometric. Cytokine levels were determined by enzyme-linked immunosorbent assay. Results Amino acid homology and phylogenetic analyses showed that the homologies of LILRB1 and LILRB3 in the chicken line 6.3 to those proteins in the chicken line 7.2 ranged between 97%–99%, while homologies between chicken and mammal proteins ranged between 13%–19%, and 13%–69%, respectively. Our findings indicate that LILRB1 and LILRB3 subdivided into two groups based on the immunoreceptor tyrosine-based inhibitory motifs (ITIM) present in the transmembrane domain. Chicken line 6.3 has two ITIM motifs of the sequence LxYxxL and SxYxxV while line 7.2 has two ITIM motifs of the sequences LxYxxL and LxYxxV. These motifs bind to SHP-2 (protein tyrosine phosphatase, non-receptor type 11) that plays a regulatory role in immune functions. Moreover, our data indicate that LILRB1 and LILRB3 associated with and activated major histocompatibility complex (MHC) class I and β2-microglobulin and induced the expression of transporters associated with antigen processing, which are essential for MHC class I antigen presentation. This suggests that LILRB1 and LILRB3 are transcriptional regulators, modulating the expression of components in the MHC class I pathway and thereby regulating immune responses. Furthermore, LILRB1 and LILRB3 activated Janus kinase2/tyrosine kinase 2 (JAK2/TYK2); signal transducer and activator of transcription1/3 (STAT1/3), and suppressor of cytokine signaling 1 genes expressed in Macrophage (HD11) cells, which induced Th1, Th2, and Th17 cytokines. Conclusion These data indicate that LILRB1 and LILRB3 are innate immune receptors associated with SHP-2, MHC class I, β2-microglobulin, and they activate the Janus kinase/signal transducer and activator of transcription signaling pathway. Thus, our study provides novel insights into the regulation of immunity and immunopathology

Simulation for Optimizing the Design of Cryogenic Stopping Cell for the IGISOL Facility at ELI-NP

The production of the exotic neutron-rich ion beams from photofission of the actinide targets in an IGISOL facility will be studied via an experimental program that will take place at the Extreme Light Infrastructure - Nuclear Physics (ELI-NP) facility. Geant4 simulation toolkit was used for optimizing the target configuration in order to maximize the rate of released photofission fragments from targets placed in a cell filled with He gas