Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria

<p>Abstract</p> <p>Background</p> <p>Reuterin produced from glycerol by <it>Lactobacillus reuteri</it>, a normal inhabitant of the human intestine, is a broad-spectrum antimicrobial agent. It has been postulated that reuterin could play a role in the probiotic effects of <it>Lb. reuteri</it>. Reuterin is active toward enteropathogens, yeasts, fungi, protozoa and viruses, but its effect on commensal intestinal bacteria is unknown. Moreover reuterin's mode of action has not yet been elucidated. Glutathione, a powerful antioxidant, which also plays a key role in detoxifying reactive aldehydes, protects certain bacteria from oxidative stress, and could also be implicated in resistance to reuterin.</p> <p>The aim of this work was to test the activity of reuterin against a representative panel of intestinal bacteria and to study a possible correlation between intracellular low molecular weight thiols (LMW-SH) such as glutathione, hydrogen peroxide and/or reuterin sensitivity. Reuterin was produced by <it>Lb</it>. <it>reuteri </it>SD2112 in pure glycerol solution, purified and used to test the minimal inhibitory (MIC) and minimal bactericidal concentrations (MBC). Hydrogen peroxide sensitivity and intracellular LMW-SH concentration were also analysed.</p> <p>Results</p> <p>Our data showed that most tested intestinal bacteria showed MIC below that for a sensitive indicator <it>Escherichia coli </it>(7.5–15 mM). Lactobacilli and <it>Clostridium clostridioforme </it>were more resistant with MIC ranging from 15 to 50 mM. No correlation between bacterial intracellular concentrations of LMW-SH, including glutathione, and reuterin or hydrogen peroxide sensitivities were found.</p> <p>Conclusion</p> <p>Our data showed that intestinal bacteria were very sensitive to reuterin and that their intracellular concentration of LMW-SH was not directly linked to their capacity to resist reuterin or hydrogen peroxide. This suggests that detoxification by LMW-SH such as glutathione is not a general mechanism and that other mechanisms are probably involved in bacterial tolerance to reuterin and hydrogene peroxide.</p

Inhibitory activity spectrum of reuterin produced by against intestinal bacteria-0

<p><b>Copyright information:</b></p><p>Taken from "Inhibitory activity spectrum of reuterin produced by against intestinal bacteria"</p><p>http://www.biomedcentral.com/1471-2180/7/101</p><p>BMC Microbiology 2007;7():101-101.</p><p>Published online 12 Nov 2007</p><p>PMCID:PMC2222629.</p><p></p>l 60 chromatography column with acetone:ethyl acetate (2:1) as eluent

Inhibitory activity spectrum of reuterin produced by against intestinal bacteria-1

<p><b>Copyright information:</b></p><p>Taken from "Inhibitory activity spectrum of reuterin produced by against intestinal bacteria"</p><p>http://www.biomedcentral.com/1471-2180/7/101</p><p>BMC Microbiology 2007;7():101-101.</p><p>Published online 12 Nov 2007</p><p>PMCID:PMC2222629.</p><p></p>l 60 chromatography column with acetone:ethyl acetate (2:1) as eluent