S gr gation de la capacit de translocation des polypeptides s cr toires dans le domaine rugueux du reticulum endoplasmique Etude sur le foie de rat

SIGLEBSE B226136U / UCL - Université Catholique de LouvainBEBelgiu

Le collag(ne 1alpha,2alpha,3alpha est-il un constituant de la fibrille de type 2? Les r actifs bifonctionnels clivables pourraient r pondre @ cette question

SIGLEBSE B226137N / UCL - Université Catholique de LouvainBEBelgiu

The Zn center of the anaerobic ribonucleotide reductase from E. coli

International audienceStrict and facultative anaerobes depend on a class III ribonucleotide reductase for their growth. These enzymes are the sole cellular catalysts for de novo biosynthesis of the deoxyribonucleotides needed for DNA chain elongation and repair. In its active form, the class III ribonucleotide reductase from Escherichia coli contains a free radical located on the G681 residue which is essential for the activation of the ribonucleotide substrate toward its reduction. The 3D structure of the homologous enzyme from bacteriophage T4 has revealed the presence of a metal center bound to four conserved cysteine residues. In this report we identify the metal of the E. coli enzyme as Zn. We show that the presence of Zn in this site protects the protein from proteolysis and prevents the formation of disulfide bridges within it. Finally, we show with the fully Zn-loaded reductase that thioredoxin or small thiols are dispensable for the formation of the glycyl radical. However, they are necessary for obtaining high turnover numbers, suggesting that they intervene in radical transfer steps subsequent to the formation of the glycyl radical

Coordination et mise en œuvre de deux expériences pilotes dans des établissements scolaires de la Communauté française en vue de renforcer l'articulation entre l'enseignement primaire et l'enseignement secondaire. Rapport de recherche intermédiaire 1re année

Recherche en éducationinfo:eu-repo/semantics/publishe

Defining the Interacting Regions between Apomyoglobin and Lipid Membrane by Hydrogen/Deuterium Exchange Coupled to Mass Spectrometry

International audienceSperm whale myoglobin can be considered as the model protein of the globin family. The pH-dependence of the interactions of apomyoglobin with lipid bilayers shares some similarities with the behavior of pore-forming domains of bacterial toxins belonging also to the globin family. Two different states of apomyoglobin bound to a lipid bilayer have been characterized by using hydrogen/deuterium exchange experiments and mass spectrometry. When bound to the membrane at pH 5.5, apomyoglobin remains mostly native-like and interacts through α-helix A. At pH 4, the binding is related to the stabilization of a partially folded state. In that case, α-helices A and G are involved in the interaction. At this pH, α-helix G, which is the most hydrophobic region of apomyoglobin, is available for interaction with the lipid bilayer because of the loss of the tertiary structure. Our results show the feasibility of such experiments and their potential for the characterization of various membrane-bound states of amphitropic proteins such as pore-forming domains of bacterial toxins. This is not possible with other high-resolution methods, because these proteins are usually in partially folded states when interacting with membranes

Coordination et mise en œuvre de deux expériences pilotes dans des établissements scolaires de la Communauté française en vue de renforcer l’articulation entre l’enseignement primaire et l’enseignement secondaire

Rapport de recherche 1ère annéeinfo:eu-repo/semantics/publishe

Adenovirus dodecahedron, as a drug delivery vector.

Stability studies show that Dds can be conveniently stored and transported, and can potentially be used for therapeutic purposes under various climates. Successful BLM delivery by Ad Dds demonstrates that the use of virus like particle (VLP) results in significantly improved drug bioavailability. These experiments open new vistas for delivery of non-permeant labile drugs

Coordination et mise en œuvre de deux expériences pilotes dans des établissements scolaires de la Communauté française en vue de renforcer l'articulation entre l'enseignement primaire et l'enseignement secondaire. Rapport de recherche 1re année

Recherche en éducationinfo:eu-repo/semantics/publishe