Chemoattraction of bone marrow-derived stem cells towards human endometrial stromal cells is mediated by estradiol regulated CXCL12 and CXCR4 expression

AbstractBone marrow derived cells engraft to the uterine endometrium and contribute to endometriosis. The mechanism by which these cells are mobilized and directed to the endometrium has not been previously characterized. We demonstrate that human endometrial stromal cells (hESCs) produce the chemokine CXCL12 and that bone marrow cells (BMCs) express the CXCL12 receptor, CXCR4. Treatment with physiological levels of estradiol (E2) induced both CXCL12 and CXCR4 expression in hESCs and BMCs, respectively. BMCs migrated towards hESCs conditioned media; a CXCR4 antagonist blocked migration indicating that CXCL12 acting through its receptor, CXCR4, is necessary for chemoattraction of BM cells to human endometrial cells. E2 increased both CXCL12 expression in endometrial cells and CXCR4 expression in BM cells, further enhancing chemoattraction. E2 induced CXCL12/CXCR4 expression in endometrium and BM, respectively, drives migration of stem cells to the endometrium. The E2-CXCL12/CXCR4 signaling pathway may be useful in determining treatments for endometrial disorders, and may be antagonized to block stem cell migration to endometriosis

Water Quality Chl-a Inversion Based on Spatio-Temporal Fusion and Convolutional Neural Network

The combination of remote sensing technology and traditional field sampling provides a convenient way to monitor inland water. However, limited by the resolution of remote sensing images and cloud contamination, the current water quality inversion products do not provide both high temporal resolution and high spatial resolution. By using the spatio-temporal fusion (STF) method, high spatial resolution and temporal fusion images were generated with Landsat, Sentinel-2, and GaoFen-2 data. Then, a Chl-a inversion model was designed based on a convolutional neural network (CNN) with the structure of 4-(136-236-340)-1-1. Finally, the results of the Chl-a concentrations were corrected using a pixel correction algorithm. The images generated from STF can maintain the spectral characteristics of the low-resolution images with the R2 between 0.7 and 0.9. The Chl-a inversion results based on the spatio-temporal fused images and CNN were verified with measured data (R2 = 0.803), and then the results were improved (R2 = 0.879) after further combining them with the pixel correction algorithm. The correlation R2 between the Chl-a results of GF2-like and Sentinel-2 were both greater than 0.8. The differences in the spatial distribution of Chl-a concentrations in the BYD lake gradually increased from July to August. Remote sensing water quality inversion based on STF and CNN can effectively achieve high frequency in time and fine resolution in space, which provide a stronger scientific basis for rapid diagnosis of eutrophication in inland lakes

HOXA10 Inhibits Kruppel-Like Factor 9 Expression in the Human Endometrial Epithelium1

Kruppel-like factor 9 (KLF9) is a zinc finger transcription factor that regulates estrogen and progesterone action by modulating the activity of progesterone receptor (PGR). The transition from proliferative to secretory endometrial epithelium involves loss of estrogen receptor/PGR expression and loss of direct response to sex steroids. HOXA10 partially mediates progesterone responsiveness in the endometrium. Here, we demonstrate that HOXA10 directly regulates KLF9 in endometrial epithelial cells and not in stromal cells. Immunohistochemistry performed on endometrial tissue obtained from normal, reproductive-age women revealed that KLF9 expression was decreased in the secretory phase of the menstrual cycle compared to the proliferative phase. In vitro, HOXA10 transfection of human endometrial epithelial cells (Ishikawa), but not stromal cells (HESC), resulted in a greater than 50% decrease in KLF9 mRNA and protein expression. Reporter constructs driven by the KLF9 promoter were repressed by cotransfection with HOXA10. Electrophoretic mobility shift assay was used to demonstrate direct binding of HOXA10 to the KLF9 promoter. Targeted mutation of the HOXA10-binding site in the KLF9 promoter resulted in loss of HOXA10 binding and loss of repression by HOXA10 in reporter assays. HOXA10 directly and selectively repressed KLF9 expression in endometrial epithelial cells. HOXA10 repression of KLF9 likely contributes to the loss of sex steroid responsiveness in secretory-phase endometrial epithelium

Direct regulation of HOXA10 by 1,25-(OH)\u3csub\u3e2\u3c/sub\u3eD\u3csub\u3e3\u3c/sub\u3e in human myelomonocytic cells and human endometrial stromal cells

Vitamin D receptor (VDR) and the functionally active form of its ligand, 1,25-(OH)2D3, have been implicated in female reproduction function and myeloid leukemic cell differentiation. HOXA10 is necessary for embryo implantation and fertility, as well as hematopoeitic development. In this study, we identified a direct role of vitamin D in the regulation of HOXA10 in primary human endometrial stromal cells, the human endometrial stromal cell line (HESC), and in the human myelomonocytic cell line, U937. Treatment of primary endometrial stromal cells, or the cell lines HESC and U937 with 1,25-(OH) 2D3 increased HOXA10 mRNA and protein expression. VDR mRNA and protein were detected in primary uterine stromal cells as well as HESC and U937 cells. We cloned the HOXA10 upstream regulatory sequence and two putative vitamin D response elements (VDRE) into luciferase reporter constructs and transfected primary stromal cells and HESC. One putative VDRE (P1: -385 to -434 bp upstream of HOXA10) drove reporter gene expression in response to treatment with 1,25-(OH)2D3. In EMSA, VDR demonstrated binding to the HOXA10 VDRE in the presence of 1,25-(OH)2D3. 1,25-(OH)2D3 up-regulates HOXA10 expression by binding VDR and interacting with a VDRE in the HOXA10 regulatory region. Direct regulation of HOXA10 by vitamin D has implications for fertility and myeloid differentiation. Copyright © 2005 by The Endocrine Society

The mediating effect of psychological flexibility on fatigue and depressive symptoms among nursing staff

Objectives To explore the relationship between depressive symptoms, fatigue and psychological flexibility, as well as their interactions on depression in Chinese nurses. Material and Methods Using convenience sampling, a cross-sectional survey of 796 nurses in municipal hospitals of Zhengzhou, Henan Province, China, was conducted. The questionnaires of Work-related Acceptance and Action Questionnaire, Center for Epidemiological Studies Depression Scale and Fatigue Assessment Instrument were used. Hierarchical regression and bootstrap methods were used to examine the mediating effect of psychological flexibility between fatigue and depression. Results More than 51.8% of the nurses were at risk of depression and 62.3% were at risk of fatigue. There was a significantly positive and moderate correlation between depression and fatigue severity, situation specificity, and consequences (r = 0.43, r = 0.24 and r = 0.31, respectively, p < 0.01). Depression was negatively correlated with psychological flexibility (r = –0.28, p < 0.01). Psychological flexibility had a negative impact on depression with the explained variance increased by 4.2% (β = –0.211, p < 0.001). The bootstrap method showed that the mediating effect of psychological flexibility accounting for 8.5% and 12.3% on fatigue and depressive symptoms, respectively. Conclusions Psychological flexibility plays a partial mediating role between the fatigue severity, consequences of fatigue and depressive symptoms of nurses. Hospital managers should improve medical staff work acceptance to alleviate their depressive symptoms

Ethylene glycol monoethyl ether (EGME) adsorption by organic matter (OM)-clay complexes: Dependence on the OM Type

The ethylene glycol monoethyl ether (EGME) adsorption method has been used as an available technique for measuring the total specific surface area (TSSA) of soils and clay-rich rocks. However, the existence of organic matters (OM) has recently been proposed to affect the accurate measurement of the TSSA. To explore the effects of OM on the TSSA evaluation of clay-rich samples, EGME adsorption experiments were performed on OM and the OM-clay minerals (OM-clay) complexes that widely exist in soil and clay-rich rocks. Two types of OM, 12-aminolauric acid (ALA) and lauric acid (LA) were used, and montmorillonite (Mt) was selected as the representative clay mineral. OM-clay complexes with OM in interlayer space or OM-clay mixture with OM on the external surface of an expanding clay mineral were prepared to investigate the influence of occurrence sites of OM on the EGME adsorption. The combined methods of X-ray diffraction (XRD) and diffuse reflectance infrared Fourier transform spectroscopy (DRIFT) were used to study the structural characteristics of the OM-clay complexes before and after EGME adsorption for revealing the EGME adsorption mechanisms. The results showed both the occurrence sites and the functional groups of OM significantly influence the EGME adsorption behaviour and TSSA for OM-clay complexes. As ALA intercalated into interlayer space of Mt, it can occupy parts of adsorption sites of EGME leading to a lower TSSA than that of Mt. While as LA located on the external surface of Mt, it affects access to the interlayer surface by the EGME and occupies parts of EGME adsorption sites of the external surface of Mt, resulting in lower adsorption capability and the slight smaller TSSA than Mt. In addition, EGME reacted strongly with LA producing excess TSSA, which brings about a great difference between LA-Mt and ALA-Mt on EGME adsorption behaviour. These fundamental results demonstrated that OM could strongly affect the EGME adsorption on the OM-Mt complexes and further influence detection of TSSA. The occurrence sites and the functional groups of OM in OM-clay complex must be considered when the EGME adsorption method is used for TSSA evaluation of such clay-rich rocks and soil samples

Bone Marrow-Derived Stem Cell (BMDSC) Transplantation Improves Fertility in a Murine Model of Asherman's Syndrome

<div><p>Asherman's Syndrome is characterized by intrauterine adhesions or fibrosis resulting as a consequence of damage to the basal layer of endometrium and is associated with infertility due to loss of normal endometrium. We have previously shown that bone marrow derived stem cells (BMDSCs) engraft the endometrium in mice and humans and Ischemia/reperfusion injury of uterus promoted BMDSCs migration to the endometrium; however, the role of BMDSCs in Asherman's syndrome has not been characterized. Here a murine model of Asherman's syndrome was created by traumatizing the uterus. We evaluate stem cell recruitment and pregnancy after BMDSCs transplantation in a model of Asherman's syndrome. In the Asheman's syndrome model, after BMDSC transplant, the Y chromosome bearing CD45-cells represented less than 0.1% of total endometrial cells. Twice the number of Y+CD45- cells was identified in the damaged uterus compared to the uninjured controls. There was no significant difference between the damaged and undamaged uterine horns in mice that received injury to a single horn. In the BMDSC transplant group, 9 of the 10 mice conceived, while only 3 of 10 in the non-transplanted group conceived (Chi-Square p = 0.0225); all mice in an uninjured control group conceived. The time to conception and mean litter size were not different between groups. Taken together, BMDSCs are recruited to endometrium in response to injury. Fertility improves after BMDSC transplant in Asherman's Syndrome mice, demonstrating a functional role for these cells in uterine repair. BMDSC transplantation is a potential novel treatment for Asherman's Syndrome and may also be useful to prevent Asherman's syndrome after uterine injury.</p></div

Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing

Radix polygalae, the dried roots of Polygala tenuifolia and P. sibirica, is one of the most well-known traditional Chinese medicinal plants. Radix polygalae contains various saponins, xanthones, and oligosaccharide esters and these compounds are responsible for several pharmacological properties. To provide basic breeding information, enhance molecular biological analysis, and determine secondary metabolite biosynthetic pathways of P. tenuifolia, we applied Illumina sequencing technology and de novo assembly. We also applied this technique to gain an overview of P. tenuifolia transcriptome from samples with different years. Using Illumina sequencing, approximately 67.2% of unique sequences were annotated by basic local alignment search tool similarity searches against public sequence databases. We classified the annotated unigenes by using Nr, Nt, GO, COG, and KEGG databases compared with NCBI. We also obtained many candidates CYP450s and UGTs by the analysis of genes in the secondary metabolite biosynthetic pathways, including putative terpenoid backbone and phenylpropanoid biosynthesis pathway. With this transcriptome sequencing, future genetic and genomics studies related to the molecular mechanisms associated with the chemical composition of P. tenuifolia may be improved. Genes involved in the enrichment of secondary metabolite biosynthesis-related pathways could enhance the potential applications of P. tenuifolia in pharmaceutical industries