Measuring Supply Chain Performance Based on SCOR: A Case Study of a Garment Company in Taiwan

The performance of supply chain is an important factor for the success of a company since it greatly affects the ability to provide customer value. Therefore, it is very important for a company to develop independent criteria to evaluate the performance, compare with competitors, and monitor the operation of a company. In the past, many companies tried to develop criteria for measuring their performance of supply chain. However, suitable criteria are hard to develop since the supply chain is generally very complex. The purpose of this study is to develop criteria to measure the performance of supply chain by using the Supply Chain Operations Reference Model (SCOR), which was shown by several previous studies to be an effective tool to develop criteria for measuring performance in diverse industries. To investigate the effectiveness of SCOR, we use the process reference model in SCOR to analyze the current state of a famous garment company’s processes and its goals, and quantify the operational performance. Results from this study show that SCOR is a very effective tool to develop performance metrics of the supply chain. Through the use of SCOR, a company can clearly define key performance indices (KPI) to improve their performance

1-Benzoyl-3-(5-quinol­yl)thio­urea

The title compound, C17H13N3OS, was obtained by the reaction of benzoyl chloride, ammonium thio­cyanate and 5-amino­quinoline in the presence of polyethyl­eneglycol-400 (PEG-400) as a phase-transfer catalyst. The compound crystallized as discrete mol­ecules linked by N—H⋯N and C—H⋯N hydrogen bonds involving all the potential donors, generating sheets parallel to (100). An intramolecular N—H⋯O bond is also present

DC-SIGN as an attachment factor mediates Japanese encephalitis virus infection of human dendritic cells via interaction with a single high-mannose residue of viral E glycoprotein

AbstractThe skin-resident dendritic cells (DCs) are thought to be the first defender to encounter incoming viruses and likely play a role in Japanese encephalitis virus (JEV) early infection. In the current study, following the demonstration of JEV productive infection in DCs, we revealed that the interaction between JEV envelope glycoprotein (E glycoprotein) and DC-SIGN was important for such infection as evidenced by antibody neutralization and siRNA knockdown experiments. Moreover, the high-mannose N-linked glycan at N154 of E glycoprotein was shown to be crucial for JEV binding to DC-SIGN and subsequent internalization, while mutation of DC-SIGN internalization motif did not affect JEV uptake and internalization. These data together suggest that DC-SIGN functions as an attachment factor rather than an entry receptor for JEV. Our findings highlight the potential significance of DC-SIGN in JEV early infection, providing a basis for further understanding how JEV exploits DC-SIGN to gain access to dendritic cells

Research of fermentation preparation technology and preliminary application for Deoxynivalenol-degrading direct inoculated microbial inoculum

Deoxynivalenol(DON)，as a secondary metabolite of some fusarium species，has been harmful to the food and feed industry.To improve spore-producing ability of Bacillus subtilis，the fermentation pH，temperature，supplemental carbon source and duration of Bacillus subtilis that efficiently degraded DON were studied in 5L-quadruple fermenters.The best fermentation conditions were determined，the fermentation pH was controlled at 7.5 and the temperature was controlled by stages，kept at 37 ℃ for first 12 hours，then raised to 39 ℃ until the end; 10 g/L molasses was added once at the 12th hour，then the fermentation was terminated at the 30th hour.Under the optimal conditions，the number of spores could reach 2.9×1010 CFU/mL and the spores’ rate could reach 96.7%.The fermentation liquid were further prepared into direct inoculated microbial inoculum and applied to remove DON from grain processing by-products; Under the condition that dry matter content in the composite material culture medium was not more than 30%，and the inoculation amount of the bacterium preparation was more than 0.1%，DON in the grain by-products could be effectively removed by fermentation and the highest degradation rate could reach 98.8%

Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virus

Humoral immune response after immunization. Sera from IIama was collected, two-fold diluted and tested by HI using LaSota as antigen. Figure S1 Amplification of VHH through a nested PCR. (A) First round PCR to separate VH from VHH. The upper 900 bp bands represent the VH-CH1-Hinge-CH2 of conventional Abs (lane 1–8). The lower 600 bp bands represent the VHH-Hinge-CH2 of HCAbs (lane 1–8). (B) VHH amplified through nested PCR using 600 bp fragment recovered from first round PCR as template (lane 1–4). M in A and B was the DL2000 DNA marker. C in A and B represent the negative control. Figure S2 PCR identification of inserted VHH. 47 clones were randomly picked to determine the library functional diversity by PCR using universal primers T7 and 3’AD (Table 1). Meanwhile, Sterile water was used as negative controls. 45 clones have amplified the 500 bp VHH fragments (lane 1–47), while negative templates control haven’t amplified any bands (lane C). M indicated the DL2000 DNA marker. Figure S3 Detection of library capacity and library titer. (A) 10-3 dilution plating of the transformed cells calculated a library capacity of 1.25 × 107 independent clones. (B) 10-5 dilution plating of the cultured library indicated a library titer of 3.45 × 108 cfu/mL. Figure S4 Deduced amino acid aligment of 10 random picked VHH. Deduced amino acid sequences were analyzed according to the Kabat numbering. Differences in the sequences are pinked, and the dash represent the missing sequences. Two hallmark Cys residues are labeled by the thick-line boxes. The four conservative hallmark residues of VHH in FR2 are labeled by the dotted line boxes. Figure S5 pGBKT7-HN bait plasmid construction. (A) PCR was carried out to amplify a truncate HN gene (without transmembrane region) from La Sota strain. M, 5000 DNA marker. 1, Truncate HN. C, Negative control. (B) A truncate HN was cloned into pGBKT7 through BamH I and Sal I. M, 5000 DNA marker. 1, Double restriction enzyme digestion of pGBKT7-HN. Figure S6 pHSIE-VHH plasmid construction. (A) 7 positive VHH fragment were amplified from recovered positive clones containing pGADT7-VHH by PCR. M, 5000 DNA marker. 1–7, VHH 1–7. C, Negative control. (B) Double restriction enzyme digestion of pHSIE-VHHs. M, 5000 DNA marker. 1–7, pHSIE-VHH 1–7. Figure S7 Western blot analysis of bait protein expression. 2 mL of Y2HGold(pGBKT7-HN) culture liquid was extracted using yeast protein extraction reagent (Takara). c-Myc tag monoclonal antibody (1:4000 dilution) was used as first antibody and HRP-labeled goat anti-mouse antibody (1:5000) was used as second antibody. The immunoreactive was visualized with cECL Plus Western blotting detection reagent (CWBIO). (DOC 1129 kb

MicroRNA-196a-5p targeting LRP1B modulates phenotype of thyroid carcinoma cells

Introduction: Thyroid cancer (TC) is a common endocrine malignancy, comprising nearly one-third of all head and neck malignancies worldwide. MicroRNAs (miRNAs) have been implicated in the malignant progression of multiple cancers; however, their contribution to thyroid diseases has not been fully explored. Material and methods: This study aimed to illustrate the regulatory mechanism of microRNA-196a-5p in TC progression and to investigate whether microRNA-196a-5p affects progression of TC cells by targeting low-density lipoprotein receptor-associated protein 1B (LRP1B). MicroRNA-196a-5p and LRP1B expression status in TC cells and normal human thyroid cells was detected by quantative reverse transcription polymerase chain reaction (qRT-PCR) and western blot. Dual-luciferase reporter assay, cell counting kit-8 (CCK-8) assay, scratch healing assay, and Transwell assay were also performed. Results: The results showed that microRNA-196a-5p expression was up-regulated and LRP1B expression was down regulated in TC cells. In addition, the upregulation of microRNA-196a-5p facilitated progression of TC cells. Silencing microRNA-196a-5p led to the opposite results. Dual-luciferase reporter assay offered evidence for microRNA-196a-5p targeting LRP1B in TC. MicroRNA-196a-5p could target LRP1B to facilitate proliferation, invasion, and migration of TC cells. Conclusion: Overall, this study revealed that microRNA-196a-5p may be a cancer-promoting microRNA that plays an important role in TC progression