Bioinformatic Analyses of Unique (Orphan) Core Genes of the Genus Acidithiobacillus: Functional Inferences and Use As Molecular Probes for Genomic and Metagenomic/Transcriptomic Interrogation

Indexación: Web of Science.Using phylogenomic and gene compositional analyses, five highly conserved gene families have been detected in the core genome of the phylogenetically coherent genus Acidithiobacillus of the class Acidithiobacillia. These core gene families are absent in the closest extant genus Thermithiobacillus tepidarius that subtends the Acidithiobacillus genus and roots the deepest in this class. The predicted proteins encoded by these core gene families are not detected by a BLAST search in the NCBI non-redundant database of more than 90 million proteins using a relaxed cut-off of 1.0e(-5). None of the five families has a clear functional prediction. However, bioinformatic scrutiny, using pI prediction, motif/domain searches, cellular location predictions, genomic context analyses, and chromosome topology studies together with previously published transcriptomic and proteomic data, suggests that some may have functions associated with membrane remodeling during cell division perhaps in response to pH stress. Despite the high level of amino acid sequence conservation within each family, there is sufficient nucleotide variation of the respective genes to permit the use of the DNA sequences to distinguish different species of Acidithiobacillus, making them useful additions to the armamentarium of tools for phylogenetic analysis. Since the protein families are unique to the Acidithiobacillus genus, they can also be leveraged as probes to detect the genus in environmental metagenomes and metatranscriptomes, including industrial biomining operations, and acid mine drainage (AMD).http://journal.frontiersin.org/article/10.3389/fmicb.2016.02035/ful

High phosphate content significantly increases apatite formation of fluoride-containing bioactive glasses

NOTICE: this is the author’s version of a work that was accepted for publication in Acta Biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Biomaterialia, [VOL 7, ISSUE 4, (2001)] DOI: 10.1016/j.actbio.2010.11.03

Scale Factor Self-Dual Cosmological Models

We implement a conformal time scale factor duality for Friedmann-Robertson-Walker cosmological models, which is consistent with the weak energy condition. The requirement for self-duality determines the equations of state for a broad class of barotropic fluids. We study the example of a universe filled with two interacting fluids, presenting an accelerated and a decelerated period, with manifest UV/IR duality. The associated self-dual scalar field interaction turns out to coincide with the "radiation-like" modified Chaplygin gas models. We present an equivalent realization of them as gauged K\"ahler sigma models (minimally coupled to gravity) with very specific and interrelated K\"ahler- and super-potentials. Their applications in the description of hilltop inflation and also as quintessence models for the late universe are discussed.Comment: v3, improved and extended version to be published in JHEP; new results added to sect.2; 4 figures; 17pg

THE INFLUENCE OF ERGONOMIC CONCEPT TO THE WORK POSTURE AND THE PHYSICAL WORK ENVIRONMENT AND ITS IMPACT ON THE WORKER PERFORMANCE (A Case Study On The Manufacturing Process Division at PT. Sinar Terang Logamjaya Bandung)

The design of reliable product needs an integrated system design and the importance of ergonomic concept. One of the references is the high performance of workers; it needs a good work posture and physical work environment to reduce accidents. Based on path analysis method, it can be concluded that most of accidents are happen due to bad work posture during the work is running and lack of support from the environment, so that the workers are not comfortable to doing their jobs. Things to do to develop high performance are environment infrastructures improvement, conducting a well plan and continue training of ergonomic concepts, so that these concepts will be a good work culture among the workers. Keywords : Ergonomic Concept, Work Posture, Physical Work Environment, Worker Performance, Path Anaysis

Pengembangan Software Automatic Double-Identification Parking System (Studi Kasus : Kampus IV Universitas Pasyndan"

Hampir di semua kampus yang ada di Kota Bandung mengalami masalah kurangnya fasilitas parkir yang dapat dilihat dari banyak kendaraan parkir di bahu jalan sekitar kampus terkait. Seperti halnya di Kampus IV Universitas Pasundan, khususnya Fakultas Teknik saat ini akan membangun tempat parkir tiga lantai untuk motor. Namun, tidak hanya tempat yang dibutuhkan, namun juga manajemen parkir yang baik, oleh karena itu dibutuhkan sistem parkir terkomputerisasi untuk mempermudah dan meminimalisir human error. Saat ini sistem parkir yang ada hanya melakukan satu identifikasi yaitu nomor polisi kendaraan yang direpresntasikan di karcis parkir. Artinya, yang memiliki karcis parkir bisa membawa kendaraan tersebut meskipun bukan pemilik. Walaupun ada SOP pengecekan STNK, namun hal ini tidak dilakukan dan hanya dilakukan saat terjadi kehilangan karcis parkir. Di Kampus IV sendiri, dengan manajemen parkir dikelola sendiri, pengecekan karcis parkir saat keluar kampus sering tidak dilakukan. Dikarenakan pembukaan portal parkir masih di triger oleh manusia bukan oleh sistem komputer. Sehingga human error seperti ini tinggi sekali dan sering terjadi kehilangan kendaraan. Diperlukan sebuah sistem parkir yang bisa meminimalisir human error baik dari mulai identifikasi kendaraan dan pemilik serta triger dari sistem untuk membuka gerbang saat masuk dan keluar. Sistem ini akan diterapkan di gedung parkir kampus IV Unpas dan diharapkan mampu memenuhi tujuan tersebut serta dengan double-identification (kendaraan dan pemilik), kehilangan kendaranan tidak akan terjadi lagi

The Newtonian Limit of Hermitian Gravity

We construct the gauge invariant potentials of Hermitian Gravity and derive the linearized equations of motion they obey. A comparison reveals a striking similarity to the Bardeen potentials of general relativity. We then consider the response to a point particle source, and discuss in what sense the solutions of Hermitian Gravity reduce to the Newtonian potentials. In a rather intriguing way, the Hermitian Gravity solutions exhibit a generalized reciprocity symmetry originally proposed by Born in the 1930s. Finally, we consider the trajectories of massive and massless particles under the influence of a potential. The theory correctly reproduces the Newtonian limit in three dimensions and the nonrelativistic acceleration equation. However, it differs from the light deflection calculated in linearized generalrelativity by 25%. While the specific complexification of general relativity by extension to Hermitian spaces performed here does not agree with experiment, it does possess useful properties for quantization and is well-behaved around singularities. Another form of complex general relativity may very well agree with experimental data.Comment: The published version in Gen. Rel. Grav. 24 pages, no figure