21 research outputs found
Evolution of the peripheral blood lymphocyte populations in multiparous rabbit does with two reproductive management rhythms
The emergence of epizootic rabbit enteropathy is leading to changes in weaning protocols in commercial rabbitries. Traditional weaning protocols are being replaced with late weaning, beyond 35 days postpartum (dpp). The main objectives of this study were to compare the peripheral blood lymphocyte populations of multiparous rabbit does under two reproductive rhythms (insemination at 11 dpp and weaning at 28 dpp, insemination at 25 dpp and weaning at 42 dpp), and to assess the influence on those of kits. Samples of peripheral blood were taken in 22 adult females and 44 of their kits at different critical times, and several lymphocytic populations were evaluated by flow cytometry. Additionally, the perirenal fat thickness of does was also measured at partum and weaning to observe if body condition correlates with lymphocyte populations. During whole lactation, counts of total, CD5 +, CD4 + and CD8 + lymphocytes of females were generally lower with weaning at 42 dpp compared to 28 dpp. Moreover, counts of total, B and CD5 + lymphocytes in rabbit does weaned at 42 dpp correlated to their body condition (+0.60 to 0.82; P<0.05), contrary to that observed in rabbit does weaned at 28 dpp. Some correlations between lymphocyte counts in both groups of does and weaning rabbits were observed. At weaning, those young rabbits weaned at 42 dpp had a significantly lower number of CD4 + lymphocytes than those weaned at 28 dpp (P<0.01). In conclusion, the 42 ddp rabbit does presented a lower number of total lymphocytes and lymphocytic subpopulations during lactation and at weaning, as well as lesser capacity of adjustment during the gestation-lactation cycle. © 2010 Elsevier B.V.This study has been supported by grants from the CEU-Cardenal Herrera University (Banco Santander/CEU-UCH: Copernicus Program (PRCEU-UCH/COOP 01/08), Generalitat Valenciana (ACOMP/2009/207 and ACOMP/2010/062) and the Comision Interministerial de Ciencia y Tecnologia (AGL2008-00273/GAN). Grants for Selena Ferrian and Irene Guerrero from Generalitat Valenciana (the Santiago Grisolia programme) and from the CEU-Cardenal Herrera University, respectively, are gratefully acknowledged.Guerrero, I.; Ferrian, S.; Blas Ferrer, E.; Pascual Amorós, JJ.; Cano, JL.; Corpa, JM. (2011). Evolution of the peripheral blood lymphocyte populations in multiparous rabbit does with two reproductive management rhythms. Veterinary Immunology and Immunopathology. 140(1-2):75-81. https://doi.org/10.1016/j.vetimm.2010.11.017S75811401-
REVIEW: II - DIET AND IMMUNITY : CURRENT STATE OF KNOWLEDGE AND RESEARCH PROSPECTS FOR THE RABBIT
[EN] Feeding plays an importan! role on the immune
system of the animals. Nutriments contained in food can have a
direct role on immunity while being used as substrates for the
cellular proliferation and !he synthesis of the effector and informan!
molecules. They can also have an indirect role: the nutritional status
acts on immunity throughout !he neuroendocrine system and sorne
nutrients (unsaturated fatty acids and vitamins A, D and E) have an
immunomodulatory action. In addition to the nutriments that it
contains, the food can affect the sensitivity of the animals to the
infections by modifying the intestinal flora, the attachment capacity
of the micro-organisms and the intestinal epithelium integrity. In
addition, sorne molecules not having a major nutritional function, such as carotenoids, can modulate the immune answer. Lastly, !he
food can be a vector for molecules or organisms that have a role on
immune system (glucans, probiotics, mycotoxins). lf the feeding can
affect the immune system, reciprocally, a stimulation immune
system (in case of infectious disease for example) modifies the
nutritional needs of the sick animal. The whole of the data
concerning the interactions between feeding and immunity obtained
in other species make it possible to propose interesting perspectives
of research in rabbit. lndeed, the control of digestive pathologies is
one of the major difficulties of the rabbit breeding and appropriate
feeding could contribute to improve immune capacity of the animals.[FR] connaissances et perspectives de recherches pour le lapin.
L'alimentation joue une role importan! sur le systéme immunitaire
des animaux. Les nutriments contenus dans l'aliment peuvent avoir
un role direct sur l'immunité en servan! de substrats pour la
prolifération cellulaire et la synthése des molécules effectrices et
informatrices. lis peuvent Ă©galement avoir un role indirect : le statut
nutritionnel agit sur l'immunité par l'intermédiaire du systéme
neuroendocrinien tandis que certains nutriments (acides gras
polyinsaturés et vitamines A, D et E) ont une action
immunomodulatrice. L'aliment peut également affecter la sensibilité
des animaux aux infections en modifiant la flore intestinale, la
capacité d'attachement des microorganismes aux entérocytes et
l'intégrité de l'épithélium intestinal. Par ailleurs, certaines molécules n'ayant pas de role nutritionnel majeur, tels que les caroténo'ides,
peuvent moduler la réponse immunitaire. Enfin, l'aliment peut servir
de vecteur a des molécules ou a des organismes qui ont un role sur
le fonctionnement du systéme immunitaire (glucanes, probiotiques,
mycotoxines). Si l'alimentation peut affecter le systéme immunitaire,
réciproquement, une stimulation mettant en jeu la réponse immune
(maladie infectieuse par exemple) modifie les besoins nutritionnels
de !'animal malade. L'ensemble des données concernant les
interactions entre alimentation et immunité obtenues sur les autres
espéces permettent de proposer des pistes de travail intéressantes
chez le lapin. En effet, la maitrise des pathologies est une des
difficultés majeures de l'élevage cunicole et une alimentation
adéquate pourrait contribuer a renforcer les défenses immunitaires
des animaux.Fortun-Lamothe, L.; Drouet-Viard, F. (2002). REVIEW: II - DIET AND IMMUNITY : CURRENT STATE OF KNOWLEDGE AND RESEARCH PROSPECTS FOR THE RABBIT. World Rabbit Science. doi:10.4995/wrs.2002.473SWORD10
Revue : I- Organisation et fonctionnement du système immunitaire : particularités chez le lapin
International audienc
REVIEW: I -THE ORGANISATION AND FUNCTIONING OF THE IMMUNE SYSTEM: PARTICULAR FEATURES OF THE RABBIT""
[EN] This article summarises the general organisation of the immune system, and the origins of its cells and their roles. The
various aspects of the immune response, innate, specific, humoral
el cell mediated, are briefly described. The immune response of the intestinal mucosa is presentad with the inductiva and effector
sites. The regulatory pathways of the immune response and the
techniques that can be used to evaluate the immune status of an
animal are also presentad.[FR] , texte rappelle l'organisation générale du systéme immunitaire,
·igine des cellules le composant et leur róle. Les différents
pects de la réaction immunitaire, naturelle et spécifique, humorale ou a médiation cellulaire, sont abordés. La réponse immune de la
muqueuse intestinale, avec présentation des sites inducteurs et
effecteurs, est plus particuliérement développée. La régulation de la
réponse immunitaire et les techniques utilisables pour évaluer le
statut immunitaire d'un animal sont également présentéesDrouet-Viard, F.; Fortun-Lamothe, L. (2002). REVIEW: I -THE ORGANISATION AND FUNCTIONING OF THE IMMUNE SYSTEM: PARTICULAR FEATURES OF THE RABBIT"". World Rabbit Science. doi:10.4995/wrs.2002.472SWORD10
Mammalian coccidiosis : natural resistance of suckling rabbits
Resume * INRA, centre de Tours Diffusion du document : INRA, centre de ToursInternational audienc
Comparaison of labelling (Ifi and Blot) of several Eimeria species with hybridoma antibodies (HAb) raised against E.intestinalis
*INRA, centre de Tours Diffusion du document : INRA, centre de ToursInternational audienc
Eimeria perforans and E. coecicola : multiplication rate and effect of the acquired protection on the oocyst output
Resume * INRA, centre de Tours Diffusion du document : INRA, centre de ToursInternational audienc
Immunogenicity of precocious lines of Eimeria magna and E.intestinalis
Resume * INRA, centre de Tours Diffusion du document : INRA, centre de ToursInternational audienc
ETUDE DE LA RESISTANCE ACQUISE PAR LA LAPINE REPRODUCTRICE IMMUNISEE AVEC UNE LIGNEE PRECOCE d'Eimeria magna ET DE SA TRANSMISSION A SA PORTEE
[EN] Fifty two coccidia free rabbit does were used in this experiment an~
26 of them were immunized by repeated per os inoculations of 10
oocysts of Eimeria magna precocious line during gestation.The
oocyst output was completely controlled after the third inoculation
and signs of disease were never observed. The serum antibodies
(Ab) were titrated by an ELISA method. In the controls, the Ab titers
were constantly below the optical density 0.25 at the wave length
405nm. In immunized does, the Ab level began to increase 4 weeks
after the first inoculation. Booster inoculations given 7 and 4 days
before parturition produced a high increase of Ab level. 26 females
received an injection of sporozoites extracts 2 and 17 days atter parturition; these injections produced a dramatic lncrease of the Ab
level compared with that of the untreated females. The serum Ab of
the weanlĂngs were titrated at 29 and 39 days of age. In all groups,
the Ab titers were very low but a slightly higher level was observed in
the goups corresponding to the immunized does. The dramatic
increase of the Ab titers of the does injected with sporozoites had no
effect on the Ab titers of their litters. To test the protectlog
transmitted by the mothers, the weanlings were inoculated with 10
oocysts of the same Eimeria magna precocious line and the oocyst
output 'fÂŞs measured. The whole excretion of oocysts was identical
(6 x 10 oocysts) in all groups, and the decrease in welght galn was
identical to that of inoculated controla. We can conclude that the
does' immunity did not protect the litter against an infection with
E.magna.[FR] Cinquante deux lapines exemptes de coccidies ont été
utĂ/isĂ©es pour cet essai, dont ;26 immunisĂ©es par os au moyen
d'inoculations fépétées de 1a9 oocystes de la souche précoce
d'Eimeria magna pendant la gestation. Aprés la troisieme
inoculation, l'excfétion d'oocystes a été indécelable, et aucun signe
de maladie n'a été observé. Les anticorps sériques (Ac) ont été titrés
par une technique ELISA. Chez les témoins, /es titres en Ac
restaient au-dessous d'une densité optique de 0,25 a la longueur
d'onde de 405nm. Chez /es meres immunisées, /e niveau des Ac
s'Ă©levait 4 semaines aprĂ©s la premiĂ©re inoculation. Les inoculatĂons
de rappel faites 7 et 4 jours avant la mise-bas ont provoqué une
élévation importante du titre en Ac. 26 feme/les ont re9u une
injection d'extraits de sporozoiles 2 et 17 jours aprés la m1se-bas;
ces injections ont provoqué une trés forte élévation du titre en Ac par rapport a celui des feme/les non traitées. Les Ac sériques des
lapereaux au sevrage ont été titfés a 29 et 39 jours. Dans tous les
groupes, les titres en Ac étaient trés bas, mais nous avons observé
un titre légerement plus élevé dans les groupes correspondant aux
meres immunisées. L'augmentation du titre en Ac chez les meres
traitées avec des extraits de sporozolfes n'a pas eu d'effet sur les
titres en Ac de leurs portées. Pour évaluer l'immuni'é_ transmise par
les meres, les lapereaux ont été inoculés avec 10 oocystes de la
souche précoce d'Eimeria magna, et l'excrétion f oocystes a été
mesurée. L'excrétion totale a été identique (6 x 10 oocystes) dans
tous les groupes, et la perte de gain de poids a été la mime que
ce/le des témoins inoculés. Nous pouvons conclure que l'immunitéDrouet-Viard, F.; Coudert, P.; Roux, C.; Licois, D.; Boivin, M. (1996). ETUDE DE LA RESISTANCE ACQUISE PAR LA LAPINE REPRODUCTRICE IMMUNISEE AVEC UNE LIGNEE PRECOCE d'Eimeria magna ET DE SA TRANSMISSION A SA PORTEE. World Rabbit Science. 04(3). doi:10.4995/wrs.1996.289SWORD04