44 research outputs found
The Endosymbiotic Coral Algae Symbiodiniaceae Are Sensitive to a Sensory Pollutant: Artificial Light at Night, ALAN
Artificial Light at Night, ALAN, is a major emerging issue in biodiversity conservation, which can negatively impact both terrestrial and marine environments. Therefore, it should be taken into serious consideration in strategic planning for urban development. While the lionâs share of research has dealt with terrestrial organisms, only a handful of studies have focused on the marine milieu. To determine if ALAN impacts the coral reef symbiotic algae, that are fundamental for sustainable coral reefs, we conducted a short experiment over a period of one-month by illuminating isolated Symbiodiniaceae cell cultures from the genera Cladocopium (formerly Clade C) and Durusdinium (formerly Clade D) with LED light. Cell cultures were exposed nightly to ALAN levels of 0.15 Îźmol quanta mâ2 sâ1 (âź4â5 lux) with three light spectra: blue, yellow and white. Our findings showed that even in very low levels of light at night, the photo-physiology of the algaeâs Electron Transport Rate (ETR), Non-Photochemical Quenching, (NPQ), total chlorophyll, and meiotic index presented significantly lower values under ALAN, primarily, but not exclusively, in Cladocopium cell cultures. The findings also showed that diverse Symbiodiniaceae types have different photo-physiology and photosynthesis performances under ALAN. We believe that our results sound an alarm for the probable detrimental effects of an increasing sensory pollutant, ALAN, on the eco-physiology of symbiotic corals. The results of this study point to the potential effects of ALAN on other organisms in marine ecosystem such as fish, zooplankton, and phytoplankton in which their biorhythms is entrained by natural light and dark cycles
Myosin XIK is a major player in cytoplasm dynamics and is regulated by two amino acids in its tail
It has recently been found that among the 17 Arabidopsis myosins, six (XIC, XIE, XIK, XI-I, MYA1, and MYA2) have a major role in the motility of Golgi bodies and mitochondria in Nicotiana benthamiana and Nicotiana tabacum. Here, the same dominant negative tail fragments were also found to arrest the movement of Gogi bodies when transiently expressed in Arabidopsis plants. However, when a Golgi marker was transiently expressed in plants knocked out in these myosins, its movement was dramatically inhibited only in the xik mutant. In addition, a tail fragment of myosin XIK could inhibit the movement of several post-Golgi organelles, such as the trans-Golgi network, pre-vacuolar compartment, and endosomes, as well as total cytoplasmic streaming, suggesting that myosin XIK is a major player in cytoplasm kinetics. However, no co-localization of myosin tails with the arrested organelles was observed. Several deletion truncations of the myosin XIK tail were generated to corroborate function with localization. All deletion mutants possessing an inhibitory effect on organelle movement exhibited a diffuse cytoplasmic distribution. Point mutations in the tail of myosin XIK revealed that Arg1368 and Arg1443 are essential for its activity. These residues correspond to Lys1706 and Lys1779 from mouse myosin Va, which mediate the inhibitory headâtail interaction in this myosin. Therefore, such an interaction might underlie the dominant negative effect of truncated plant myosin tails and explain the mislocalization with target organelles
Investigation of the Degradation Behavior of Cyclophosphamide by Catalytic Ozonation Based on Mg(OH)2
Metal hydroxides, owing to their catalytic active sites for the decomposition of O3 to Reactive Oxygen Species (ROS), have been adapted for catalytic ozonation of micropollutants in wastewater. In this study, commercial Mg (OH)2 was used for the degradation of cyclophosphamide (CYP) by ozone. The crystal phase was confirmed by X-ray powder Diffraction (XRD). Percent degradation of 10 ppm CYP after 30 min by O3 and Mg (OH)2/O3 was 56 and 93, respectively, suggesting enhanced decomposition of O3 to ROS by the catalyst. The presence of ROS was further confirmed using pCBA as a probe, which showed that the concentration of ROS was eight times higher in the presence of Mg (OH)2/O3 than O3 alone. Catalytic ozonation experiments in the presence of scavengers showed that OH· radicals play a significant role in the degradation of CYP. The catalyst was found to be reusable for at least three cycles without significant loss in degradation efficiency. To study the compatibility of Mg (OH)2 for wastewater treatment applications, synthetic effluent was spiked with CYP and subjected to ozonation by Mg(OH)2/O3. The TOC of CYP before and after the treatment showed that Mg (OH)2/O3 not only degrades CYP but also mineralizes to a certain extent unlike O3 alone
Agricultural Irrigation with Effluent â What should we be Worried About?
Policy regarding effluent water and reclamation aims to prevent environmental pollution while proposing an alternative water resource. Water makes up 99â99.9% of raw wastewater. Thus extracting organic and inorganic matter from water is a must. Worldwide, but especially in developed countries, great effort has been made to reuse wastewater, and it is becoming a reliable alternative source. Israel is the world leader in water reuse, allocating 85% of effluent water for agricultural irrigation. As such, it constitutes a âliving laboratoryâ in which to study the implications of the intensive use of treated wastewater for agricultural irrigation, leading to research and legislation regarding effluent quality and regulation. Effluent produced in Israel is subject to severe regulations and standards and is considered suitable for every use except drinking water. It is mostly allocated for agricultural irrigation with no restrictions. The irrigated lands are close to natural water sources, and therefore water leaching from the fields infiltrate those sources, becoming part of the water cycle. A group of persistent and toxic nano- and micro-organic contaminants, including pharmaceutical residues, flows to water-treatment plants from hospitals, industry, agriculture and especially the domestic sector. These contaminants' chemical structure, characterized by aromatic rings and double bonds, makes them especially persistent; they are resistant to conventional biological treatment, used as a secondary treatment. As a result, the effluent that leaves the treatment plants, which is considered to be of high quality, actually contains pharmaceutical residues. After secondary and tertiary treatment, these persistent chemical residues can still be found in surface water, groundwater and agricultural products. Pharmaceutical residues in effluent allocated for agricultural irrigation are undesirable. Expansion of the monitoring system for those contaminants, improvement of the tertiary treatment, and implementation of advanced technologies for decomposition and removal of pharmaceutical contaminants are thus needed
Pharmaceutical Transformation Products Formed by Ozonation—Does Degradation Occur?
The efficiency of an advanced oxidation process (AOP) using direct and indirect ozonation for the removal of pharmaceutical residues from deliberately spiked deionized water was examined. Both direct and indirect ozonation demonstrated 34% to 100% removal of the parent compounds. However, based on the products’ chemical structure and toxicity, we suggest that despite using accepted and affordable ozone and radical concentrations, the six parent compounds were not fully degraded, but merely transformed into 25 new intermediate products. The transformation products (TPs) differed slightly in structure but were mostly similar to their parent compounds in their persistence, stability and toxicity; a few of the TPs were found to be even more toxic than their parent compounds. Therefore, an additional treatment is required to improve and upgrade the traditional AOP toward degradation and removal of both parent compounds and their TPs for safer release into the environment
Pharmaceutical Transformation Products Formed by OzonationâDoes Degradation Occur?
The efficiency of an advanced oxidation process (AOP) using direct and indirect ozonation for the removal of pharmaceutical residues from deliberately spiked deionized water was examined. Both direct and indirect ozonation demonstrated 34% to 100% removal of the parent compounds. However, based on the productsâ chemical structure and toxicity, we suggest that despite using accepted and affordable ozone and radical concentrations, the six parent compounds were not fully degraded, but merely transformed into 25 new intermediate products. The transformation products (TPs) differed slightly in structure but were mostly similar to their parent compounds in their persistence, stability and toxicity; a few of the TPs were found to be even more toxic than their parent compounds. Therefore, an additional treatment is required to improve and upgrade the traditional AOP toward degradation and removal of both parent compounds and their TPs for safer release into the environment
Trace Organic Compound Removal from Wastewater Reverse-Osmosis Concentrate by Advanced Oxidation Processes with UV/O3/H2O2
Advanced technologies, such as reverse osmosis (RO), allow the reuse of treated wastewater for direct or indirect potable use. However, even highly efficient RO systems produce ~10–15% highly contaminated concentrate as a byproduct. This wastewater RO concentrate (WWROC) is very rich in metal ions, nutrients, and hard-to-degrade trace organic compounds (TOrCs), such as pharmaceuticals, plasticizers, flame retardants, and detergents, which must be treated before disposal. WWROC could be up to 10 times more concentrated than secondary effluent. We examined the efficiency of several advanced oxidation processes (AOPs) on TOrC removal from a two-stage WWROC matrix in a pilot wastewater-treatment facility. WWROC ozonation or UV irradiation, with H2O2 addition, demonstrated efficient removal of TOrCs, varying between 21% and over 99% degradation, and indicating that radical oxidation (by HO·) is the dominant mechanism. However, AOPs are not sufficient to fully treat the WWROC, and thus, additional procedures are required to decrease metal ion and nutrient concentrations. Further biological treatment post-AOP is also highly important, to eliminate the degradable organic molecules obtained from the AOP
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Nature of Flow and Gas Dynamics Below Spreading Ponds
The El Rio Spreading Grounds, an area of artificial recharge, lies within a regional groundwater depression. It consists of ten small ponds surrounded by eight production wells. A deliberate tracer experiment using sulfur hexafluoride (SF6) and an isotope of helium (3He) was conducted to determine groundwater flow. The tracer was continuously injected into one pond for one week prior to a ~50 day period of no recharge. At the time of the experiment, the artificial recharge rate through the highly permeable alluvial sediments which underlie the ponds was ~4 m day-1 and the water table was ~12 m below the ground surface. Tracer breakthrough curves from the production wells indicate that the plume remained near (within 500 m) the injection site during the 18 month experiment. Its movement was influenced by a number of factors. First, variable groundwater production in the production wells cause frequent changes in the water level, and thus, rates and direction of the plume flow respond to local groundwater cones of depression. Second, additional recharge events, which were free of tracer, create groundwater mounds that block the transport of the tracer to specific production wells. Noble gas concentrations in the groundwater were much higher than in the pond due to the dissolution of trapped air. However, significant retardation (absolute and relative) of the gas tracers was not detected. Breakthrough curves of SF6 and 3He at two nearby production wells (within ~10 m of the pond) were very similar and suggest that nonequilibrium gas transfer was occurring between the percolating water and trapped air. At one well screened between 50 and 90 m below ground, both tracers were detected after 5 days and reached a maximum at ~24 days. Despite potential dilution caused by mixing within the production well, the maximum concentration observed at this well was ~33% of the mean pond concentration. More than 50% of the SF6 recharged was recovered by the production wells during this 18 month experiment. The experiment demonstrates that at artificial recharge sites with high infiltration rates and moderately deep water tables, transport times between recharge locations and wells determined with gas tracer experiments are reliable
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Gas transport below artificial recharge ponds: insights from dissolved noble gases and a dual gas (SF6 and 3He) tracer experiment.
A dual gas tracer experiment using sulfur hexafluoride (SF6) and an isotope of helium (3He) and measurements of dissolved noble gases was performed at the El Rio spreading grounds to examine gas transport and trapped air below an artificial recharge pond with a very high recharge rate (approximately 4 m day(-1)). Noble gas concentrations in the groundwater were greater than in surface water due to excess air formation showing that trapped air exists below the pond. Breakthrough curves of SF6 and 3He at two nearby production wells were very similar and suggest that nonequilibrium gas transfer was occurring between the percolating water and the trapped air. At one well screened between 50 and 90 m below ground, both tracers were detected after 5 days and reached a maximum at approximately 24 days. Despite the potential dilution caused by mixing within the production well, the maximum concentration was approximately 25% of the mean pond concentration. More than 50% of the SF6 recharged was recovered by the production wells during the 18 month long experiment. Our results demonstrate that at artificial recharge sites with high infiltration rates and moderately deep water tables, transport times between recharge locations and wells determined with gas tracer experiments are reliable
Recommended from our members
Gas transport below artificial recharge ponds: insights from dissolved noble gases and a dual gas (SF6 and 3He) tracer experiment.
A dual gas tracer experiment using sulfur hexafluoride (SF6) and an isotope of helium (3He) and measurements of dissolved noble gases was performed at the El Rio spreading grounds to examine gas transport and trapped air below an artificial recharge pond with a very high recharge rate (approximately 4 m day(-1)). Noble gas concentrations in the groundwater were greater than in surface water due to excess air formation showing that trapped air exists below the pond. Breakthrough curves of SF6 and 3He at two nearby production wells were very similar and suggest that nonequilibrium gas transfer was occurring between the percolating water and the trapped air. At one well screened between 50 and 90 m below ground, both tracers were detected after 5 days and reached a maximum at approximately 24 days. Despite the potential dilution caused by mixing within the production well, the maximum concentration was approximately 25% of the mean pond concentration. More than 50% of the SF6 recharged was recovered by the production wells during the 18 month long experiment. Our results demonstrate that at artificial recharge sites with high infiltration rates and moderately deep water tables, transport times between recharge locations and wells determined with gas tracer experiments are reliable