8 research outputs found
Novel expression patterns of phosphatidylinositol 3-hydroxy kinase in nodulated Medicago spp. plants
A cDNA clone encoding a phosphatidylinositol 3-kinase (PtdIns 3-kinase) has been characterized from Medicago truncatula, Mtpi3k, that is highly homologous to their counterparts from soybean (over 84%). The results suggest the presence of at least two genes coding PtdIns 3-kinases in M. truncatula. Mtpi3k transcript levels increased in nodules, compared with non-infected roots. Strikingly, Mtpi3k mRNA accumulated in young elongating stems at higher levels than that observed in other organs. Enhanced transcription of genes coding PtdIns 3-kinases might occur in tissues experiencing a high degree of vesicle trafficking and cell elongation
Interaction of maize actin-depolymerising factor with actin and phosphoinositides and its inhibition of plant phospholipase C
Regulation of the pollen-specific actin-depolymerizing factor LIADF1
Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LlADF1. Its actin binding and depolymerizing activity is pH sensitive, inhibited by certain phosphoinositides, but not controlled by phosphorylation. Compared with its F-actin binding properties, its low activity in depolymerization assays has been used to explain why pollen ADF decorates F-actin in pollen grains. This low activity is incompatible with a role in increasing actin dynamics necessary to promote pollen tube growth. We have identified a plant homolog of actin-interacting protein, AIP1, which enhances the depolymerization of F-actin in the presence of LlADF1 by 60%. Both pollen ADF and pollen AIP1 bind F-actin in pollen grains but are mainly cytoplasmic in pollen tubes. Our results suggest that together these proteins remodel actin filaments as pollen grains enter and exit dormancy
Phosphatidylinositol 3- and 4-phosphate modulate actin filament reorganization in guard cells of day flower
Changes in starch and inositol 1,4,5-trisphosphate levels and auxin transport are interrelated in graviresponding oat ( Avena sativa ) shoots
This study was conducted to unravel a mechanism for the gravitropic curvature response in oat ( Avena sativa ) shoot pulvini. For this purpose, we examined the downward movement of starch-filled chloroplast gravisensors, differential changes in inositol 1,4,5-trisphosphate (IP 3 ) levels, transport of indole-3-acetic acid (IAA) and gravitropic curvature. Upon gravistimulation, the ratio for IAA levels in lower halves versus those in upper halves (L/U) increased from 1.0 at 0 h and reached a maximum value of 1.45 at 8 h. When shoots were grown in the dark for 10 d, to deplete starch in the chloroplast, the gravity-induced L/U of IAA was reduced to 1.0. N -naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA), both auxin transport inhibitors, significantly reduced the amount of gravitropic curvature and gravity-induced lateral IAA transport, but did not reduce the gravity-induced late change in the L/U ratio of IP 3 levels. U73122, a specific phospholipase C (PLC) inhibitor, decreased gravity-induced curvature. Because U73122 reduced the ratio of L/U of IAA imposed by gravistimulation, it is clear that IAA transport is correlated with changes in IP 3 levels upon gravistimulation. These results indicate that gravistimulation-induced differential lateral IAA transport may result from the onset of graviperception in the chloroplast gravisensors coupled with gravity-induced asymmetric changes in IP 3 levels in oat shoot pulvini.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74234/1/j.1365-3040.2006.01584.x.pd