Contribution to the Improvement of Vegetative Propagation by Greenhouse Cuttings of Odorous Verbena (Aloysia citrodora)

Currently several species are threatened with extinction due to climatic factors, population pressure and the strong global demand for a continuous and uniform supply of fragrant, aromatic and medicinal plants. The multiplication and domestication of these species remains the only way to save them from extinction, our study is within the framework of propagation by cuttings of odorous verbena (Aloysia citrodora). In fact, some parameters influencing the success of cuttings have been studied, namely the nature of the substrate, the effect of certain rooting products and the position of the cutting in relation to the mother plant (basal or apical), the tests were carried out in a tunnel greenhouse at the ibn tofail university, faculty of sciences kenitra, Morroco. Out of 144 trials, the success rate of cuttings according to the type of substrate was 91.66%, 75% and 83.33% respectively for substrates S1 (soil only), S2 (1/2 sand and 1/2 compost) and S3 (1/3 soil; 1/3 compost and 1/3 peat). For the three products, we didn’t make combinations. but we followed the effect of each product alone, For the treatment effect, 77%, 0% and 69% respectively were obtained for Product 1 (auxin), Product 2 (based on mineral matter and amino acids) and Product 3 (seaweed extract). With regard to the position of the cutting, high percentages were obtained for cuttings from the basal position

Recent Trends on Domestic, Agricultural and Industrial Wastewaters Treatment Using Microalgae Biorefinery System

In addition to producing bioenergy and molecules with high added value, microalgae have been recognized as an efficient microorganism for wastewater treatment. However, a major obstacle preventing its widespread use is the high energy cost of pretreatment, cultivation and downstream processes. Different types of wastewaters have been tested as culture mediums for microalgal biorefinery system. This review gives a summary of the most used microalgae strains for wastewater treatment, as well as information on the physical and chemical characteristics of domestic, agricultural, and industrial wastewaters. It also discusses wastewater pretreatment techniques, nutrient uptake and removal, biomass production and biomolecules productivities. There is also discussion on how microalgae remove contaminants from wastewater. Additionally, the problems and restrictions of microalgae-based wastewater treatment are explored, and recommendations are made for additional study and advancement. This literature review demonstrates that microalgae monoculture systems have proven to be beneficial as an innovative wastewater treatment technology, due to its high efficiencies in pollutant removals and biomolecule production; however, the upstream and downstream treatment pose a limit to industrialize the process. Until now, there has been no conventional design of the wastewater treatment process using microalgae in the biorefinery system, which constitutes a huge gap to assess a real life cycle assessment (LCA) and techno economic analysis (TEA)

Combination of <i>Microalgae</i> Method, Decantation, and Filtration for Domestic Wastewater Treatment

Phycoremediation of wastewater with microalgae is a viable option and is considered a process for cleaning up toxic waste using microalgae or macroalgae. Most water is modified by its use and must be treated before discharge. Given this situation, and following the example of other researchers around the world, our study focuses on the filtration method and combines it with the microalgae method to treat domestic wastewater. The aim of our work is to study the effects of using the microalgae system in combination with the decontamination and filtration system to reduce the nutrient content of domestic wastewater. The coupling of the two methods produced very significant results. However, the removal efficiencies for the filtered effluent increased to 86.34%, 100%, and 91.12% for COD, ammonia, and phosphate, respectively. The algae treatment offers an ecologically safe and less expensive system for nutrient removal and eliminates the need for tertiary treatment, which refers to the filtered treatment effluent, allowing us to conclude that the Chlorella vulgaris species has a very interesting influence on dissolved oxygen and that it had a very remarkable effect on COD, with a maximum reduction that reached 80%. The results obtained show that the phosphate content of the treated wastewater was significantly reduced during the cultivation period. In time, a decrease in solids was observed within the microalgae treatment system, influenced by the use of two different types of microalgae and the incorporation of the filtration system, which is based on the use of biosorption of methylene blue by biomass. The parameters analyzed in this study are hydrogen potential (pH), ammonia (NH3), phosphate ion (PO43−), chemical oxygen demand (COD), electrical conductivity (EC), total solids (TS), total dissolved solids (TDS), total suspended solids (TSS), nitrates, and dissolved oxygen (DO)

In Vitro Studies on the Antimicrobial and Antioxidant Activities of Total Polyphenol Content of Cynara humilis from Moulay Yacoub Area (Morocco)

In Morocco, Cynara humilis L. is used in traditional medicine. The objective of this research was to research the antioxidant and antimicrobial properties of hydroethanolic extracts from the C. humilis plant&rsquo;s leaves and roots. The content of polyphenols and flavonoids was evaluated using Folin&ndash;Ciocalteu&rsquo;s and aluminum chloride assays. Two techniques were used to evaluate antioxidant properties: antioxidant capacity in total (TAC) and 2,2-diphenyl-1-picrylhdrazyl (DPPH). In antimicrobial assays, five pathogenic microbial strains were studied including two Escherichia coli, one coagulase-negative Staphylococcus and Klebsiella pneumoniae, and one Candida albicans, by two techniques: agar disk diffusion and microdilution. Leaves had a greater content of flavonoids 27.07 mg QE/g of extract and the polyphenols 38.84 mg GAE/g of extract than root 24.39 mg QE/g of extract and 29.39 mg GAE/g of extract, respectively. The TAC test value of the 0.77 mg AAE/g extract in the leaf extract was found to be significantly greater than that of the 0.60 mg EAA/g extract in the root extract. The DPPH antioxidant assay IC50 values of the root and leaf extract were 0.23 and 0.93 &micro;g/mL, respectively. C. humilis extracts showed an antimicrobial effect against all tested strains, the inhibitory zone (DIZ) have values in the range between 12 and 15 mm. Moreover, the root extract showed the lowest minimum inhibitory concentration (MIC) against coagulase-negative Staphylococcus with an IC50 value of 6.25 mg/mL. The higher content of flavonoids and polyphenols in the hydroethanolic extracts of C. humilis leaves and roots demonstrates that they have a significant antimicrobial and antioxidant effect, as found in this study

Antimicrobial and Antioxidant Properties of Total Polyphenols of Anchusa italica Retz

Anchusa italica Retz has been used for a long time in phytotherapy. The aim of the present study was to determine the antioxidant and antibacterial activities of extracts from the leaves and roots of Anchusa italica Retz. We first determined the content of phenolic compounds and flavonoids using Folin&ndash;Ciocalteu reagents and aluminum chloride (AlCl3). The antioxidant activity was determined using three methods: reducing power (FRAP), 2.2-diphenyl-1-picrylhydrazyl (DPPH), total antioxidant capacity (TAC). The antimicrobial activity was investigated against four strains of Escherichia coli, two strains of Klebsiella pneumoniae and coagulase-negative Staphylococcus, and one fungal strain of Candida albicans. The results showed that the root extract was rich in polyphenols (43.29 mg GAE/g extract), while the leave extract was rich in flavonoids (28.88 mg QE/g extract). The FRAP assay showed a strong iron reduction capacity for the root extract (IC50 of 0.11 &micro;g/mL) in comparison to ascorbic acid (IC50 of 0.121 &micro;g/mL). The DPPH test determined an IC50 of 0.11 &micro;g/mL for the root extract and an IC50 of 0.14 &micro;g/mL for the leaf extract. These values are low compared to those for ascorbic acid (IC50 of 0.16 &micro;g/mL) and BHT (IC50 0.20 &micro;g/mL). The TAC values of the leaf and root extracts were 0.51 and 0.98 mg AAE/g extract, respectively. In vitro, the extract showed inhibitory activity against all strains studied, with diameters of zones of inhibition in the range of 11.00&ndash;16.00 mm for the root extract and 11.67&ndash;14.33 mm for the leaf extract. The minimum inhibitory concentration was recorded for the leaf extract against E. coli (ATB:57), corresponding to 5 mg/mL. Overall, this research indicates that the extracts of Anchusa italica Retz roots and leaves exert significant antioxidant and antibacterial activities, probably because of the high content of flavonoids and polyphenols