eSciDoc.PubMan - flyer

Information flyer about the eSciDoc solution PubMan

A gas-liquid solid phase peptide and protein sequenator

A new miniaturized protein and peptide sequenator has been constructed which uses gas phase reagents at the coupling and cleavage steps of the Edman degradation. The sample is embedded in a matrix of Polybrene dried onto a porous glass fiber disc located in a small cartridge-style reaction cell. The protein or peptide, though not covalently attached to the support, is essentially immobile throughout the degradative cycle, since only relatively apolar, liquid phase solvents pass through the cell. This instrument can give useful sequence data on as little as 5 pmol or protein, can perform extended sequence runs (greater than 30 residues) on subnanomole quantities of proteins purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and can sequence hydrophobic peptides to completion. The sequenator is characterized by a high repetitive yield during the degradation, low reagent consumption, low maintenance requirements, and a degradative cycle time of only 50 min using a complete double cleavage program

Research & Development User Interface Engineering (UIE)

Flyer about User Interface Engineering (UIE) at the MPDL

Die huwelik as identiteitsmerker in die Ou Testament

Marriage as identity marker in the Old Testament The formularies used for  consecrating marriages in the Nederduitsch Hervormde Church reflect this church’s view on matrimony. As the biblical bases of the formularies are deficient, new ways of exploring biblical information on this subject should be followed. This article proposes that data on marriage in the Bible always be used in conjunction with other concepts to form theological constructs to outline who God is and who his people are. It is always intended to be an identity marker to the members of the church. In the three Genesis cycles of Genesis 11:10-25:11; 25:12-35:29 and 36:1-50:26 heirship, marriage and land are used in an integrated construct to indicate the  identity of the post-exilic community in Yehud. In the penitential prayers of Ezra 9:6-15 and Nehemia 9:5b-37 the concepts law, land and marriage are jointly used to depict the identity of the “real” Israel. The conclusion drawn from this investigation is that the Bible does not present models for marriage, but rather theological constructs to understand the relationship with the Lord in metaphorical terms and to reflect on the meaning of everyday life of that relationship including matrimony under ever changing social circumstances