Transcriptional Targeting of B Cells for Induction of Peripheral CD8 T Cell Tolerance

Abstract Several mechanisms are in place to neutralize autoimmune CD8 T cells by tolerance induction. Developing self-specific CD8 T cells are eliminated in the thymus by Ag-presenting epithelial and dendritic cells (DCs). However, CD8 T cells escaping thymic central tolerance can also be inactivated by tolerance mechanisms in peripheral organs. In contrast to DCs, the role of B cells in generating CD8 T cell tolerance is not well-characterized. To investigate this question in more detail, we transcriptionally targeted Ag to B cells using B cell-specific retroviral vectors in vivo. Although Ag expression could be detected in B cells of thymus, lymph nodes, and spleen, B cells were unable to induce central tolerance of CD8 thymocytes. In contrast, in peripheral organs, we could identify clonal deletion and functional inhibition (anergy) of CD8 T cells as tolerance-inducing mechanisms. Although Ag expressed by B cells was acquired and cross-presented by DCs, B cells were also sufficient to tolerize CD8 T cells directly. These findings suggest exploitation of B cells for Ag-specific immunotherapy of CD8 T cell-mediated autoimmune diseases

Análise de polimorfismo de fragmentos de DNA envolvidos na síntese da cadeia beta do receptor de células T em indivíduos de diferentes etnias

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Análise de polimorfismo de fragmentos de DNA envolvidos na síntese da cadeia beta do receptor de células T em indivíduos de diferentes etnias

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La correspondencia de Valencia : diario de noticias : eco imparcial de la opinión y de la prensa: Año XLVI Número 19038 - 1923 Junio 26

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Análise de polimorfismos de fragmentos de DNA envolvidos na síntese da cadeia beta do receptor de células T em indivíduos com artrite reumatóide

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Análise dos polimorfismos G54D e IVS do gene MBL2 em indivíduos com anemia falciforme

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