Impact of genotype, age of tree and environmental temperature on androgenesis induction of Aesculus hippocastanum L.

Influence of ten different genotypes, age of trees and environmental temperature on induction of androgenesis and apperance albino horse chestnut embryos were studied. Efficiency of in vitro androgenesis via anther and microspore culture had been investigated. Microspores and anthers were used from the same closed flower bud. Androgenic response of different genotypes was measured and compared. Anther induction rates were from 5 to 37.6%, depending on genotype. The number ofembryos per isolated anther varied between 0.5 to 5.0 embryos in anther culture, while in microspore culture varied between 3.0 to 27 embryos, depending on genotype. A microspore culture was 5 - 6 times efficient than anther culture for same genotype. Age of the trees had no influence on androgenesis induction. Temperature of about 4 - 5°C was optimal for androgenic embryo induction. Albino horse chestnut embryos phenomenon depended on genotype. The number of albino appearing in anther washigher than in microspore culture. The same correlation of appearing albino was observed between short and long day. Flow cytogenetic analysis of androgenic embryos originating from anther and microspore culture was done after a first generation of regenerants. All androgenic embryos, the first generation from microspore culture were haploid, while 50% of the regenerants originating from anther culture were haploid, and the other half diploid

Environmental impact quantification and correlation between site location and contents and structure of Tansy

The aim of this work was to quantify the most significant impact from the polluted environment and to review the correlation between pollution indicators and the content and structures of Tanacetum vulgare L. (Tansy). Heavy metals as mercury, lead, cadmium, chromium and nickel are considered as pollution indicators. The micro and macro elements concentration of S, P, K, Ca, Mg, Na, Fe, Si, Al and Mn were tested too. The concentration of the essential oil was analyzed as indicator of Tansy environmental adaptability. Essential oils are practically agents which represents correlation between plant and environment. The type of Tansy was selected for laboratory research, because it belongs to most speeded urban flora and vegetation, in which the imperative to adapt is very strong. All those parameters was monitored on two different locations: industrial zone (anthropogenic) and park area (non anthropogenic) for comparison, as zero stage of the environment, before pollution. The diferences were also recorded in the morphological and anatomical structure and chlorophyll a and b content of the Tansy and they were caused by differences of cumulative environmental conditions, with dominant efects of the contamination degree of the location, soil type and microclimate.Key words: Anthropogenic factors, correlation, environmental impact, environmental zero stage, quantification

Environmental study of heavy metals influence on soil and Tansy (Tanacetum vulgare L.)

The aim of this work was to define the correlation between heavy metal (mercury, lead, cadmium, chrome and nickel) concentration changes (in soil, leaf, stem, root and essential oil in Tansy) and type and distance from source pollution. The concentration was monitored on different locations: 1) Chemical industry panevo (with accident situation); 2) Panevo industrial zone; 3) highway; 4) Ada Ciganlija recreation zone and 5) Topider park. Mercury (Hg) concentration was analyzed as a functionof time, starting from accident situation from 1999 to 2008. Hg had maximum concentration of 131 200 mg/kg at a soil depth of 0 - 15 cm. After three, six and nine years, linear accident Hg concentrations insoil decreased from 85 400 via 41 060 to 106.0 mg/kg. Mercury concentration which results in the location where accident occurred showed that 6 years was necessary for concentration to drop below the limits and for revitalization of standard industrial Tansy vegetation. Concentrations of Hg in the Tansy plants from contaminated site were 5, 10, 100 and 200 times greater than in industrial zone, highway, Ada Ciganlija and Topider, respectively. The highest amount of lead (Pb) was in leaves (14.1mg/kg) and in essential oil (0.7 mg/kg) of Tansy near the highway. However, Pb concentration in soil and plant was decreased with square of highway distance

Differences and Similarities among Rural Tourism in Slovenia and Serbia – Perceptions of Local Tourism Workers

This paper deals with the development of rural areas in Slovenia and Serbia and gives some differences/similarities among these two countries based on perception of 492 tourism work - ers. The article making a series of proposals to guide the future research agenda. The main aim of the paper is to point out the benefits of rural tourism development and it’s similarities and differences in Slovenia and Serbia. The data for this study were collected using Dwyer and Kim’s (2003) Integrated Model of Destination Competitiveness to observe Slovenia’s and Serbia’s rural tourism differences/similarities. Determinants were assessed using a survey evaluating 24 indicators (demand and supporting factors), based upon a Likert Scale.Rad se bavi razvojem ruralnih područja u Sloveniji i Srbiji i prikazuje neke razlike/sličnosti između ove dve zemlje na osnovu percepcije 492 turističkih radnika. U radu se analiziraju glavni doprinosi ruralnog turizma i donosi niz predloga za buduća istraživanja. Glavni cilj rada je ukazati na pozitivne aspekte razvoja ruralnog turizma, kao i sličnosti i razlike Slovenije i Srbije. Podaci za ovu studiju su prikupljeni pomoću Dvajer i Kimovog (2003) Integrisanog modela konkurentnosti destinacije kojim se posmatraju ključne razlike/sličnosti ruralnog turizma Slovenije i Srbije. Determinante su obrađene pomoću ankete na bazi 24 indikatora zasnovanih na Likertovoj skali

Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)

An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragaevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragaevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragaevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent

The Linkage between Traditional Food and Loyalty of Tourists to the Rural Destinations

Gastronomy tourism can be regarded as a travel to a destination for cultural purposes with experiences of unique and traditional foods at the destination. Based on this, the main hypothesis is set. It follows that traditional food is one of the main reasons for loyalty of tourists. The paper is based on empirical research carried out amongst 328 foreign tourists from eight countries who visited six traditional farm houses (Salaši) in rural destination of Vojvodina (Serbia) in August 2015. The purpose of the study is to investigate the role and importance of traditional food in tourist satisfaction and the extent to which traditional food affects positive attitude and loyalty of the tourists about the rural destination. Analysis of the findings revealed that the traditional food is the attribute that most affected the overall rural tourism experience in Vojvodina (Serbia). It is concluded in the paper that traditional food is important contributor to tourist satisfaction and that satisfied tourists are always pleased to return to a destination

Effect of activated charcoal, abscisic acid and polyethylene glycol on maturation, germination and conversion of Aesculus hippocastanum androgenic embryos

The influence of activated charcoal (AC), abscisic acid (ABA) and polyethylene glycol (PEG) on the maturation and conversion of horse chestnut (Aesculus hippocastanum L.) androgenic embryos weretested. Androgenic embryos originating from microspores and anther culture were maturated over 90 days. Androgenic embryos on media containing PEG (50 g l-1), in combination with AC (1 g l-1) showed arapid development of embryos in the cotyledonary stage and lowered percentage of abnormal structures. The best results of androgenic microspore embryo germination were observed on media supplemented with AC alone (99%) and in combination with PEG (100%). Also, the greatest number of androgenic microspore plants (18%) and androgenic anther plants (12%) were formed on media enriched with 1 % AC. Lowest germination percentages of 37 and 39% in microspore culture and 33 and38% in anther culture were obtained on maturation media with ABA 20 mg l-1 alone and in combination with AC 1g l-1. Flow cytometric analysis showed that most of the androgenic embryos were haploid, corresponding to their microspore origin, while half of these became diploid after maturation for 90 days. All regenerants originating from microspore culture were haploid immediately after germination, but only 10% embryos re ained haploidity after 3 years subculturing, while 10.5% were diploid, 73.5% tetraploid and 6% octaploid on hormone-free medium. Unlike those from anther culture, after 3 years of subculturing on hormone-free medium, there were no haploid regenerant from anther culture, while8.5% were diploid, 81% tetraploid and 10.5% octaploid

Variability and bimodal distribution of size in uninuclear microspores of Aesculus flava Marshall

Projekat ministarstva br. 14302