15 research outputs found
Mapping the human amylase gene cluster on the proximal short arm of chromosome 1 using a highly informative (CA)n repeat
The human amylase gene cluster includes a (CA)n repeat sequence immediately upstream of the [gamma]-actin pseudogene associated with the AMY2B gene. Analysis of this (CA)n repeat by PCR amplification of genomic DNA from the 40 families of the Centre d'Etude du Polymorphisme Humain (CEPH) reference panel revealed extensive polymorphism. A total of six alleles with (CA)n lengths of 16-21 repeats were found. The average heterozygosity for this polymorphism was 0.70. Multipoint linkage analysis showed that the amylase gene cluster is located distal to the nerve growth factor [beta]-subunit gene (NGFB) and is within 1 cM of the anonymous locus D1S10. The amylase (CA)n repeat provides a convenient marker for both the physical and the genetic maps of human chromosome 1p.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28584/1/0000391.pd
Taql and Mspl RFLPs are detected by the human 2,3-biphosphoglycerate mutase (BPGM) cDNA
No abstract availabl
MspI RFLP detected by a ZNF-40 cDNA sequence
Source/Description: 1.6 kb EcoRI fragment from a 5' cDNA
clone (designated 4b) of the human ZNF-40 locus. ZNF-40
(MBP-1) (PRDII-BFI) encodes the class I major
histocompatibility complex (MHC) enhancer binding protein 1
(2)
Cell-free DNA approaches for cancer early detection and interception
Rapid advancements in the area of early cancer detection have brought us closer to achieving the goals of finding cancer early enough to treat or cure it, while avoiding harms of overdiagnosis. We evaluate progress in the development of early cancer detection tests in the context of the current principles for cancer screening. We review cell-free DNA (cfDNA)-based approaches using mutations, methylation, or fragmentomes for early cancer detection. Lastly, we discuss the challenges in demonstrating clinical utility of these tests before integration into routine clinical care