Control of aromatic biosynthesis in Neurospora crassa

Control of aromatic biosynthesi

The biochemical genetics of certain structural genes of the regulation enzymic function 3-deoxy-D-arabino-heptulosonate 7-Phosphate synthetase

Regulation of DAHP synthetas

The Far-Infrared Properties of Spatially Resolved AKARI Observations

We present the spatially resolved observations of IRAS sources from the Japanese infrared astronomy satellite AKARI All-Sky Survey during the performance verification (PV) phase of the mission. We extracted reliable point sources matched with IRAS point source catalogue. By comparing IRAS and AKARI fluxes, we found that the flux measurements of some IRAS sources could have been over or underestimated and affected by the local background rather than the global background. We also found possible candidates for new AKARI sources and confirmed that AKARI observations resolved IRAS sources into multiple sources. All-Sky Survey observations are expected to verify the accuracies of IRAS flux measurements and to find new extragalactic point sources.Comment: 11 pages, 7 figures, accepted publication in PASJ AKARI special issu

Thermal conductivity measurement of liquids in a microfluidic device

A new microfluidic-based approach to measuring liquid thermal conductivity is developed to address the requirement in many practical applications for measurements using small (microlitre) sample size and integration into a compact device. The approach also gives the possibility of high-throughput testing. A resistance heater and temperature sensor are incorporated into a glass microfluidic chip to allow transmission and detection of a planar thermal wave crossing a thin layer of the sample. The device is designed so that heat transfer is locally one-dimensional during a short initial time period. This allows the detected temperature transient to be separated into two distinct components: a short-time, purely one-dimensional part from which sample thermal conductivity can be determined and a remaining long-time part containing the effects of three-dimensionality and of the finite size of surrounding thermal reservoirs. Identification of the one-dimensional component yields a steady temperature difference from which sample thermal conductivity can be determined. Calibration is required to give correct representation of changing heater resistance, system layer thicknesses and solid material thermal conductivities with temperature. In this preliminary study, methanol/water mixtures are measured at atmospheric pressure over the temperature range 30–50°C. The results show that the device has produced a measurement accuracy of within 2.5% over the range of thermal conductivity and temperature of the tests. A relation between measurement uncertainty and the geometric and thermal properties of the system is derived and this is used to identify ways that error could be further reduced

Analysis and in situ hybridization of cryptic satellites in Hordeum arizonicum

Three satellites, one (H1) on the heavy side of the main band of Hordeum arizonicum DNA and two (L1, L2) on the lighter side were purified using preparative silver-cesium sulphate density gradients. The native and the reassociated satellite DNAs were analysed in terms of buoyant densities and thermal dissociation. In cesium chloride gradients the H1 and L1 satellites formed single peaks corresponding to buoyant densities of 1.700 and 1.701 g · cm-3 respectively while the L2 satellite gave two peaks (1.680 and 1.661 g · cm-3). The H1 satellite showed three thermal components (Tm=82.5 °C, 87 °C and 91.5 °C) while the L1 and L2 had three (86.5, 92, 97.5 °C) and two (86, 95 °C) respectively. The H1 satellite was localized on the nuclei and chromosomes. The distribution of H1 onto approximately on third of the complement may reflect the genome specific origin of this satellite