Detection and isolation of exotic Newcastle disease virus from field-collected flies.

Flies were collected by sweep net from the vicinity of two small groups of "backyard" poultry (10-20 chickens per group) that had been identified as infected with exotic Newcastle disease virus (family Paramyxoviridae, genus avulavirus, ENDV) in Los Angeles County, CA, during the 2002-2003 END outbreak. Collected flies were subdivided into pools and homogenized in brain-heart infusion broth with antibiotics. The separated supernatant was tested for the presence of ENDV by inoculation into embryonated chicken eggs. Exotic Newcastle disease virus was isolated from pools of Phaenicia cuprina (Wiedemann), Fannia canicularis (L.), and Musca domestica L., and it was identified by hemagglutination inhibition with Newcastle disease virus antiserum. Viral concentration in positive pools was low (<1 egg infectious dose50 per fly). Isolated virus demonstrated identical monoclonal antibody binding profiles as well as 99% sequence homology in the 635-bp fusion gene sequence compared with ENDV recovered from infected commercial egg layer poultry during the 2002 outbreak

Managing manure and conserving predators helps control flies in caged-layer poultry systems

As the rural-urban interface expands, controlling flies has become increasingly important on California poultry farms. Manure management is a critical component of keeping fly populations in check. Recent research demonstrates that the dry pad left behind after manure cleanouts in cagedlayer poultry systems aids manure drying because of the elevation and improved airflow. Most mites and beetles that prey on fly eggs and larvae are removed in a cleanout, although predator populations require longer than flies to recover. Leaving undisturbed manure (with a larger number of predators) adjacent to recently removed manure did not improve fly control significantly in open-sided layer houses, but might be more important in fully enclosed houses

Managing manure and conserving predators helps control flies in caged-layer poultry systems

As the rural-urban interface expands, controlling flies has become increasingly important on California poultry farms. Manure management is a critical component of keeping fly populations in check. Recent research demonstrates that the dry pad left behind after manure cleanouts in cagedlayer poultry systems aids manure drying because of the elevation and improved airflow. Most mites and beetles that prey on fly eggs and larvae are removed in a cleanout, although predator populations require longer than flies to recover. Leaving undisturbed manure (with a larger number of predators) adjacent to recently removed manure did not improve fly control significantly in open-sided layer houses, but might be more important in fully enclosed houses

Detection and isolation of exotic Newcastle disease virus from field-collected flies.

Flies were collected by sweep net from the vicinity of two small groups of "backyard" poultry (10-20 chickens per group) that had been identified as infected with exotic Newcastle disease virus (family Paramyxoviridae, genus avulavirus, ENDV) in Los Angeles County, CA, during the 2002-2003 END outbreak. Collected flies were subdivided into pools and homogenized in brain-heart infusion broth with antibiotics. The separated supernatant was tested for the presence of ENDV by inoculation into embryonated chicken eggs. Exotic Newcastle disease virus was isolated from pools of Phaenicia cuprina (Wiedemann), Fannia canicularis (L.), and Musca domestica L., and it was identified by hemagglutination inhibition with Newcastle disease virus antiserum. Viral concentration in positive pools was low (<1 egg infectious dose50 per fly). Isolated virus demonstrated identical monoclonal antibody binding profiles as well as 99% sequence homology in the 635-bp fusion gene sequence compared with ENDV recovered from infected commercial egg layer poultry during the 2002 outbreak