Larval Ecology of Some Lower Michigan Black Flies (Diptera: Simuliidae) With Keys to the Immature Stages

The species composition, succession, and seasonal abundance of -immature simuliids ocmrrhg in the Rose Lake Wildlife Research Area in lower Michigan are presented. Selected physical and chemical characteristics of streams in the above area were examined and compared in relation to faunal distributions. Comparisons of species differences between permanent and temporary streams were made utilizing the functional group concept based on feeding mechanisms. Keys and illustrations are presented for the identiiication of larvae and pupae of four genera (Prosimulium, Simulium, Stegopterna, Cnephia) and 19 species of Simuliidae known to occur in lower Michigan. Two species, Cnephia ornithophilia and Simulium vemum, were recorded for the first time in Michigan

Search for Large Rapidity Gap Events in e^+ e^- Annihilation

We investigate the cross-section for the production of a low-mass colour-singlet cluster in $e^+e^-$ annihilation with a large rapidity gap between the colour-singlet cluster and the other jets. It is argued that such events are the cross-channel analogue of large-rapidity-gap events in deep-inelastic scattering, and therefore could in principle be used to investigate the analytic continuation of the BFKL pomeron to the positive-$t$ kinematic regime, where one would expect the trajectory to pass through glueball states. The cross section can be calculated in perturbative QCD, so that the infrared scale arising from non-perturbative effects, which prevents an exponential fall-off with rapidity gap in the case of deep-inelastic scattering, is absent in $e^+ e^-$ annihilation. Correspondingly, the cross section for such events decreases rapidly with increasing rapidity gap.Comment: LATEX file - 21 pages + 15 figure

Genetic Taster Status as a Mediator of Neural Activity and Swallowing Mechanics in Healthy Adults

As part of a larger study examining relationships between taste properties and swallowing, we assessed the influence of genetic taster status (GTS) on measures of brain activity and swallowing physiology during taste stimulation in healthy men and women. Twenty-one participants underwent videofluoroscopic swallowing study (VFSS) and functional magnetic resonance imaging (fMRI) during trials of high-intensity taste stimuli. The precisely formulated mixtures included sour, sweet-sour, lemon, and orange taste profiles and unflavored controls. Swallowing physiology was characterized via computational analysis of swallowing mechanics plus other kinematic and temporal measures, all extracted from VFSS recordings. Whole-brain analysis of fMRI data assessed blood oxygen responses to neural activity associated with taste stimulation. Swallowing morphometry, kinematics, temporal measures, and neuroimaging analysis revealed differential responses by GTS. Supertasters exhibited increased amplitude of most pharyngeal movements, and decreased activity in the primary somatosensory cortex compared to nontasters and midtasters. These preliminary findings suggest baseline differences in swallowing physiology and the associated neural underpinnings associated with GTS. Given the potential implications for dysphagia risk and recovery patterns, GTS should be included as a relevant variable in future research regarding swallowing function and dysfunction

Proteomic analysis of tomato (Lycopersicon esculentum) pollen

In flowering plants, pollen grains are produced in the anther and released to the external environment with the primary function of delivering sperm cells to the female gametophyte. This study was conducted to identify proteins in tomato pollen and to analyse their roles in relation to pollen function. Tomato is an important crop which is grown worldwide and is an excellent experimental system. Proteins were extracted from pollen, separated by two-dimensional gel electrophoresis (2-DE), and identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and peptide mass fingerprinting. Of the 960 spots observed on Colloidal Coomassie Blue (CCB)-stained 2-DE gels, 190 were selected for analysis. Of these, 158 spots, representing 133 distinct proteins, were identified by searching the NCBInr and Expressed Sequence Tag databases. The identified proteins were classified based on designated functions and the majority included those involved in defence mechanisms, energy conversions, protein synthesis and processing, cytoskeleton formation, Ca(2+) signalling, and as allergens. A number of proteins in tomato pollen were similar to those reported in the pollen of other species; however, several additional proteins with roles in defence mechanisms, metabolic processes, and hormone signalling were identified. The potential roles of the identified proteins in the survival strategy of the small, independent, two-celled pollen grain of tomato, and subsequently in pollen germination and tube growth are discussed

Proteome profile and functional classification of proteins in Arabidopsis thaliana (Landsberg erecta) mature pollen

Proteome analysis of mature Arabidopsis thaliana (Landsberg erecta ecotype) pollen was conducted using two-dimensional gel electrophoresis and mass spectrometry. A total of 960 spots were resolved on pH 4–7 IPG strips and 110 distinct proteins were identified from 150 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, defense-related mechanisms, calcium-binding and signaling, cytoskeletal formation, pollen allergens, glycine-rich proteins (GRPs), and late embryogenesis abundant (LEA) proteins. These proteins potentially play important roles in pollen function at maturity and during subsequent germination and tube growth. Some of the proteins identified were related to known pollen-specific transcripts, while some were similar to proteins found in the seed. In this study, 66 new proteins were identified which were not reported in two other recent studies on Arabidopsis pollen, 17 proteins were common in all three studies, and 35 or 26 proteins reported here had an overlap with one or the other two studies. These differences may be attributed to the methods of protein extraction, spot selection for analysis, and the ecotype used. Together, the three studies provide a broad spectrum of the Arabidopsis pollen proteome

Death-associated Protein Kinase-1 Expression and Autophagy in Chronic Lymphocytic Leukemia Are Dependent on Activating Transcription Factor-6 and CCAAT/Enhancer-binding Protein-β

Expression of DAPK1, a critical regulator of autophagy and apoptosis, is lost in a wide variety of tumors, although the mechanisms are unclear. A transcription factor complex consisting of ATF6 (an endoplasmic reticulum-resident factor) and C/EBP-β is required for the IFN-γ-induced expression of DAPK1. IFN-γ-induced proteolytic processing of ATF6 and phosphorylation of C/EBP-β are obligatory for the formation of this transcriptional complex. We report that defects in this pathway fail to control growth of chronic lymphocytic leukemia (CLL). Consistent with these observations, IFN-γ and chemotherapeutics failed to activate autophagy in CLL patient samples lacking ATF6 and/or C/EBP-β. Together, these results identify a molecular basis for the loss of DAPK1 expression in CLL

Alpha-Vacua, Black Holes, and AdS/CFT

The Schwarzschild, Schwarzschild-AdS, and Schwarzschild-de Sitter solutions all admit freely acting discrete involutions which commute with the continuous symmetries of the spacetimes. Intuitively, these involutions correspond to the antipodal map of the corresponding spacetimes. In analogy with the ordinary de Sitter example, this allows us to construct new vacua by performing a Mottola-Allen transform on the modes associated with the Hartle-Hawking, or Euclidean, vacuum. These vacua are the `alpha'-vacua for these black holes. The causal structure of a typical black hole may ameliorate certain difficulties which are encountered in the case of de Sitter alpha-vacua. For Schwarzschild-AdS black holes, a Bogoliubov transformation which mixes operators of the two boundary CFT's provides a construction of the dual CFT alpha-states. Finally, we analyze the thermal properties of these vacua.Comment: 40 pages REVTeX and AMSLaTeX, 17 black&white eps figures. v3: references added. v4: details of the pinch singularity avoidance for the string quantization of the Rindler space toy model have been added in both the body of the paper and in a new 7 page appendix. Other clarifications and references added. This is the version accepted for publication in Class. Quant. Gra