Brief of \u3cem\u3eAmici Curiae\u3c/em\u3e Professors Joshua A. Douglas and Michael E. Solimine, Election Law Scholars, in Support of Petitioners

Professor Joshua A. Douglas and Professor Michael E. Solimine are election law experts who have a particular interest in the procedural aspects of election litigation. Professors Douglas and Solimine are filing this brief because they have a keen interest in ensuring that the federal courts employ the proper procedure in election law cases, as doing so helps to resolve these disputes in a manner that best comports with the unique aspects of the electoral system. This brief explains why district courts should not use the pleading standard from Bell Atlantic Corp. v. Twombly, 550 U.S. 544 (2007) and Ashcroft v. Iqbal, 556 U.S. 662 (2009), in making the threshold determination whether to refer a redistricting case to a three-judge district court. Relying on Professor Douglas’s and Professor Solimine’s experience and expertise in this area, it describes the history of the three-judge district court and explains the strong legal and policy reasons why Congress intended for three-judge district courts to resolve redistricting cases. The single district judge here improperly dismissed this case without referring it to a three-judge district court

FACTORS RELATED TO FECAL ESTROGENS AND FECAL TESTOSTERONE IN CALIFORNIA SPOTTED OWLS

We estimated concentrations of fecal reproductive steroid metabolites in free-ranging California Spotted Owls (Strix occidentalis occidentalis) during the breeding season. We collected fresh fecal samples (n= 142) from 65 individual owls in the Sierra Nevada during April–August of 2001. We developed and validated radioimmunoassay procedures to quantify fecal estrogen metabolites and fecal testosterone metabolites. We used an information- theoretic approach to identify factors that might influence fecal estrogen (E), fecal testosterone (T), and fecal estrogen:testosterone ratio (E:T ratio) levels during the owl’s breeding season. We hypothesized that factors related to sampling procedures, owl characteristics (sex, reproductive status), and habitat might influence fecal reproductive steroid levels. Our analyses suggested that sampling factors and owl characteristics, but not habitat variables, were related to fecal reproductive steroid levels in Spotted Owls. Our most supported models explained \u3c30% of the observed variation. Fecal testosterone levels were higher in male Spotted Owls than females, whereas E:T ratios were higher in females compared to males. High fecal estrogens were correlated with high fecal glucocorticoids in nonbreeding Spotted Owls, whereas fecal estrogens and fecal glucocorticoids were not related in breeding birds. Sampling influenced fecal reproductive steroid measures, and bias from small-mass fecal samples might partially explain these relationships. Noninvasive measurements of fecal reproductive steroids might be useful for sex determination and reproductive assessment of free-ranging Spotted Owls. However, more research is needed to understand the variability we observed in sex steroids before this technique can be effective in conservation studies

Phase identifications in crud from commercial boiling-water reactors at the Idaho National Laboratory by transmission electron microscopy

Summarizes results of two studies of "crud" at the INL. All data is from INL/EXT-06-11742 and INL/JOU-06-11507 and has been previously released for publication

Evolution of brown carbon in wildfire plumes

Particulate brown carbon (BrC) in the atmosphere absorbs light at subvisible wavelengths and has poorly constrained but potentially large climate forcing impacts. BrC from biomass burning has virtually unknown lifecycle and atmospheric stability. Here, BrC emitted from intense wildfires was measured in plumes transported over 2 days from two main fires, during the 2013 NASA SEAC4RS mission. Concurrent measurements of organic aerosol (OA) and black carbon (BC) mass concentration, BC coating thickness, absorption Ångström exponent, and OA oxidation state reveal that the initial BrC emitted from the fires was largely unstable. Using back trajectories to estimate the transport time indicates that BrC aerosol light absorption decayed in the plumes with a half-life of 9 to 15 h, measured over day and night. Although most BrC was lost within a day, possibly through chemical loss and/or evaporation, the remaining persistent fraction likely determines the background BrC levels most relevant for climate forcing

The many streams of the Magellanic Stream

We present results from neutral hydrogen (HI) observations of the tip of the Magellanic Stream (MS), obtained with the Arecibo telescope as a part of the on-going survey by the Consortium for Galactic studies with the Arecibo L-band Feed Array. We find four large-scale, coherent HI streams, extending continously over a length of 20 degrees, each stream possessing different morphology and velocity gradients. The newly discovered streams provide strong support for the tidal model of the MS formation by Connors et al. (2006), which suggested a spatial and kinematic bifurcation of the MS. The observed morphology and kinematics suggest that three of these streams could be interpreted as a 3-way splitting of the main MS filament, while the fourth stream appears much younger and may have originated from the Magellanic Bridge. We find an extensive population of HI clouds at the tip of the MS. Two thirds of clouds have an angular size in the range 3.5'--10'. We interpret this as being due to thermal instability, which would affect a warm tail of gas trailing through the Galactic halo over a characteristic timescale of a few Myrs to a few hundred Myrs. We show that thermal fragments can survive in the hot halo for a long time, especially if surrounded by a <10^6 K halo gas. If the observed clumpy structure is mainly due to thermal instability, then the tip of the MS is at a distance of ~70 kpc. A significant fraction of HI clouds at the tip of the MS show multi-phase velocity profiles, indicating the co-existence of cooler and warmer gas.Comment: Accepted by Ap

Diverse and tissue-enriched small RNAs in the plant pathogenic fungus, Magnaporthe oryzae

<p>Abstract</p> <p>Background</p> <p>Emerging knowledge of the impact of small RNAs as important cellular regulators has prompted an explosion of small transcriptome sequencing projects. Although significant progress has been made towards small RNA discovery and biogenesis in higher eukaryotes and other model organisms, knowledge in simple eukaryotes such as filamentous fungi remains limited.</p> <p>Results</p> <p>Here, we used 454 pyrosequencing to present a detailed analysis of the small RNA transcriptome (~ 15 - 40 nucleotides in length) from mycelia and appressoria tissues of the rice blast fungal pathogen, <it>Magnaporthe oryzae</it>. Small RNAs mapped to numerous nuclear and mitochondrial genomic features including repetitive elements, tRNA loci, rRNAs, protein coding genes, snRNAs and intergenic regions. For most elements, small RNAs mapped primarily to the sense strand with the exception of repetitive elements to which small RNAs mapped in the sense and antisense orientation in near equal proportions. Inspection of the small RNAs revealed a preference for U and suppression of C at position 1, particularly for antisense mapping small RNAs. In the mycelia library, small RNAs of the size 18 - 23 nt were enriched for intergenic regions and repetitive elements. Small RNAs mapping to LTR retrotransposons were classified as LTR retrotransposon-siRNAs (LTR-siRNAs). Conversely, the appressoria library had a greater proportion of 28 - 35 nt small RNAs mapping to tRNA loci, and were classified as tRNA-derived RNA fragments (tRFs). LTR-siRNAs and tRFs were independently validated by 3' RACE PCR and northern blots, respectively.</p> <p>Conclusions</p> <p>Our findings suggest <it>M. oryzae </it>small RNAs differentially accumulate in vegetative and specialized-infection tissues and may play an active role in genome integrity and regulating growth and development.</p