Gaming machine density is correlated with rates of help-seeking for problem gambling: a local area analysis in Victoria, Australia

Local environment plays an important role in understanding gambling as a public health issue. This study uses help-seeking as an outcome measure for a local area analysis of problem gambling in Victoria, Australia. We used a cross-sectional ecological design to investigate the extent to which gaming industry and demographic, economic, and social factors are associated with rates of telephone and face-to-face counselling for problem gambling at the local government area level. Electronic gaming machine density was independently correlated with both types of help-seeking, with a range of local factors controlled. This study supports previous research that has consistently found an association between gaming machine density and problem gambling, using gaming machine expenditure as a proxy measure of harm. We build on previous work by confirming that this relationship exists when gambling harm is measured through two types of help-seeking

Kovesi and the Formal and Material Elements of Concepts

Published in Philosophia, December 2011, Volume 39, Issue 4, pp 699-720. doi: 10.1007/s11406-011-9305-x</p

Crisis? What crisis? Myth and reality in the debate on a ageing Australia

The Commonwealth Government’s Intergenerational Report 2002_03claims that Australia’s ageing population poses a serious challenge for responsible fiscal policy in the longer term, a claim repeated in subsequent official pronouncements. We reject this claim, pointing to its rhetorical nature and to the lack of systematic sensitivity analysis of many of the critical variables. We conclude that the ageing ‘crisis’ is largely mythical

Crisis? What crisis? Myth and reality in the debate on a ageing Australia

The Commonwealth Government’s Intergenerational Report 2002_03claims that Australia’s ageing population poses a serious challenge for responsible fiscal policy in the longer term, a claim repeated in subsequent official pronouncements. We reject this claim, pointing to its rhetorical nature and to the lack of systematic sensitivity analysis of many of the critical variables. We conclude that the ageing ‘crisis’ is largely mythical. Copyright. Monash University and the author/

Super-multiplexed fluorescence microscopy via photostability contrast

Fluorescence microscopy is widely used to observe and quantify the inner workings of the cell. Traditionally, multiple types of cellular structures or biomolecules are visualized simultaneously in a sample by using spectrally distinct fluorescent labels. The wide emission spectra of most fluorophores limits spectral multiplexing to four or five labels in a standard fluorescence microscope. Further multiplexing requires another dimension of contrast. Here, we show that photostability differences can be used to distinguish between fluorescent labels. By combining photobleaching characteristics with a novel unmixing algorithm, we resolve up to three fluorescent labels in a single spectral channel and unmix fluorescent labels with nearly identical emission spectra. We apply our technique to organic dyes, autofluorescent biomolecules and fluorescent proteins. Our approach has the potential to triple the multiplexing capabilities of any digital widefield or confocal fluorescence microscope with no additional hardware, making it readily accessible to a wide range of researchers.Antony Orth, Richik N. Ghosh, Emma R. Wilson, Timothy Doughney, Hannah Brown, Philipp Reineck, Jeremy G. Thompson and Brant C. Gibso

Super-multiplexed fluorescence microscopy via photostability contrast

Fluorescence microscopy is widely used to observe and quantify the inner workings of the cell. Traditionally, multiple types of cellular structures or biomolecules are visualized simultaneously in a sample by using spectrally distinct fluorescent labels. The wide emission spectra of most fluorophores limits spectral multiplexing to four or five labels in a standard fluorescence microscope. Further multiplexing requires another dimension of contrast. Here, we show that photostability differences can be used to distinguish between fluorescent labels. By combining photobleaching characteristics with a novel unmixing algorithm, we resolve up to three fluorescent labels in a single spectral channel and unmix fluorescent labels with nearly identical emission spectra. We apply our technique to organic dyes, autofluorescent biomolecules and fluorescent proteins. Our approach has the potential to triple the multiplexing capabilities of any digital widefield or confocal fluorescence microscope with no additional hardware, making it readily accessible to a wide range of researchers