The expanding toolkit of translating ribosome affinity purification

Translating ribosome affinity purification is a method initially developed for profiling mRNA from genetically defined cell types in complex tissues. It has been applied both to identify target molecules in cell types that are important for controlling a variety of behaviors in the brain, and to understand the molecular consequences on those cells due to experimental manipulations, ranging from drugs of abuse to disease-causing mutations. Since its inception, a variety of methodological advances are opening new avenues of investigation. These advances include a variety of new methods for targeting cells for translating ribosome affinity purification by features such as their projections or activity, additional tags and mouse reagents increasing the flexibility of the system, and new modifications of the method specifically focused on studying the regulation of translation. The latter includes methods to assess cell type-specific regulation of translation in specific subcellular compartments. Here, I provide a summary of these recent advances and resources, highlighting both new experimental opportunities and areas for future technical development.</jats:p

Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons

AbstractWe developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of its effectiveness in capturing mRNA from these cells. We also report a translational profile of these neurons which may be of use to investigators studying the gene expression of these cells. Finally, we have provided the line to Jackson Laboratories for distribution and use in future studies.</jats:p

Ionospheric E-region Irregularities Produced by Non-linear Coupling of Unstable Plasma Waves

Ionospheric E region irregularities produced by nonlinear coupling of unstable plasma wave

A Hybrid Analysis for Security Protocols with State

Cryptographic protocols rely on message-passing to coordinate activity among principals. Each principal maintains local state in individual local sessions only as needed to complete that session. However, in some protocols a principal also uses state to coordinate its different local sessions. Sometimes the non-local, mutable state is used as a means, for example with smart cards or Trusted Platform Modules. Sometimes it is the purpose of running the protocol, for example in commercial transactions. Many richly developed tools and techniques, based on well-understood foundations, are available for design and analysis of pure message-passing protocols. But the presence of cross-session state poses difficulties for these techniques. In this paper we provide a framework for modeling stateful protocols. We define a hybrid analysis method. It leverages theorem-proving---in this instance, the PVS prover---for reasoning about computations over state. It combines that with an "enrich-by-need" approach---embodied by CPSA---that focuses on the message-passing part. As a case study we give a full analysis of the Envelope Protocol, due to Mark Ryan

In-flight transition measurement on a 10 deg cone at Mach numbers from 0.5 to 2.0

Boundary layer transition measurements were made in flight on a 10 deg transition cone tested previously in 23 wind tunnels. The cone was mounted on the nose of an F-15 aircraft and flown at Mach numbers room 0.5 to 2.0 and altitudes from 1500 meters (5000 feet) to 15,000 meters (50,000 feet), overlapping the Mach number/Reynolds number envelope of the wind tunnel tests. Transition was detected using a traversing pitot probe in contact with the surface. Data were obtained near zero cone incidence and adiabatic wall temperature. Transition Reynolds number was found to be a function of Mach number and of the ratio of wall temperature to adiabatic all temperature. Microphones mounted flush with the cone surface measured free-stream disturbances imposed on the laminar boundary layer and identified Tollmien-Schlichting waves as the probable cause of transition. Transition Reynolds number also correlated with the disturbance levels as measured by the cone surface microphones under a laminar boundary layer as well as the free-stream impact

WR146 - observing the OB-type companion

We present new radio and optical observations of the colliding-wind system WR146 aimed at understanding the nature of the companion to the Wolf-Rayet star and the collision of their winds. The radio observations reveal emission from three components: the WR stellar wind, the non-thermal wind-wind interaction region and, for the first time, the stellar wind of the OB companion. This provides the unique possibility of determining the mass-loss rate and terminal wind velocity ratios of the two winds, independent of distance. Respectively, these ratios are determined to be 0.20+/-0.06 and 0.56+/-0.17 for the OB-companion star relative to the WR star. A new optical spectrum indicates that the system is more luminous than had been believed previously. We deduce that the ``companion'' cannot be a single, low luminosity O8 star as previously suggested, but is either a high luminosity O8 star, or possibly an O8+WC binary system.Comment: 9 pages, 5 figures, ftp://fto.drao.nrc.ca/pub/smd/wr146/accepted.ps.gz To be published in Monthly Notices of the Royal Astronomical Societ

Experimental Persistence Probability for Fluctuating Steps

The persistence behavior for fluctuating steps on the $Si(111)$ $(\sqrt3 \times \sqrt3)R30^{0} - Al$ surface was determined by analyzing time-dependent STM images for temperatures between 770 and 970K. The measured persistence probability follows a power law decay with an exponent of $\theta=0.77 \pm 0.03$. This is consistent with the value of $\theta= 3/4$ predicted for attachment/detachment limited step kinetics. If the persistence analysis is carried out in terms of return to a fixed reference position, the measured persistence probability decays exponentially. Numerical studies of the Langevin equation used to model step motion corroborate the experimental observations.Comment: LaTeX, 11 pages, 4 figures, minor changes in References sectio