Inhibition of Fructose-1,6-bisphosphatase by Aminoimidazole Carboxamide Ribotide Prevents Growth of Salmonella enterica purH Mutants on Glycerol

The enzyme fructose-1,6-bisphosphatase (FBP) is key regulatory point in gluconeogenesis. Mutants of Salmonella enterica lacking purH accumulate 5-amino-4-imidazole carboxamide ribotide (AICAR) and are unable to utilize glycerol as sole carbon and energy sources. The work described here demonstrates this lack of growth is due to inhibition of FBP by AICAR. Mutant alleles of fbp that restore growth on glycerol encode proteins resistant to inhibition by AICAR and the allosteric regulator AMP. This is the first report of biochemical characterization of substitutions causing AMP resistance in a bacterial FBP. Inhibition of FBP activity by AICAR occurs at physiologically relevant concentrations and may represent a form of regulation of gluconeogenic flux in Salmonella enterica

S.S.I. Recipients Forfeit Benefits When Traveling Abroad

On December 11, 1978, in Califano v. Aznavorian, 99 S.Ct. 471 (1978), the Supreme Court unanimously upheld the constitutionality of §1611(f) of the Social Security Act (42 U.S.C. §1382(f)). Though §1611(f) denies benefits to Supplemental Security Income (SSI) recipients for any month that the recipient spends entirely outside of the United States, the Court concluded that the provision was not an impermissible burden on the right of international travel as guaranteed by the Fifth Amendment

The Utility of Trouble: Maximizing the Value of Our Human Services Dollars

Outlines recommendations to standardize service delivery areas and consolidate area offices of the state's seven largest human services agencies, as well as to close antiquated institutions. Projects benefits such as improved accessibility and savings

Greater Wheeling Regional Plan: Survey Results

The Wheeling Area Strategic Plan included a process for public involvement. Originally, it was to have involved holding open forums and in-person stakeholder meetings. The sessions had been scheduled for early Spring, but they were delayed and eventually cancelled because of the COVID-19 pandemic. Instead, we conducted a web-based survey to gather ideas and opinions from people from across the region

Directed evolution: new parts and optimized function

Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understanding, directed evolution is a powerful complement to ‘rational’ engineering approaches. Aided by clever selection schemes, directed evolution has generated new parts for genetic circuits, cell–cell communication systems, and non-natural metabolic pathways in bacteria

Information Technology Use by Local Governments in the Northeast: Assessment and Needs

How local governments are using information technology is important to understand if extension teaching on local government and economic development issues is to be effective. This study uses results from surveys of local officials in New York, Pennsylvania, and West Virginia to examine the potential for delivering extension programs to local officials through information technology. The responses suggest that local officials predominantly prefer face-to-face training, but many are willing to try distance education. In addition, even though many governments are using the Internet, there still are significant numbers who do not yet have access to these technologies