Evaluation of the Human IgG Antibody Response to Aedes albopictus Saliva as a New Specific Biomarker of Exposure to Vector Bites

Aedes-borne viruses like dengue and chikungunya are a major problem in Reunion Island. Assessing exposure to Aedes bites is crucial to estimating the risk of pathogen transmission. Currently, the exposure of populations to Aedes albopictus bites is mainly evaluated by entomological methods which are indirect and difficult to apply on a large scale. Recent findings suggest that evaluation of human antibody responses against arthropod salivary proteins could be useful in assessing exposure to mosquito bites. The results indicate that 88% of the studied population produce IgG to Ae. albopictus saliva antigens in Reunion Island and show that this biomarker can detect different levels of individual exposure. In addition, little cross-reactivity is observed with Aedes aegypti saliva, suggesting that this could be a specific marker for exposure to Aedes albopictus bites. Taken together, these results suggest that antibody responses to saliva could constitute a powerful immuno-epidemiological tool for evaluating exposure to Aedes albopictus and therefore the risk of arbovirus infection

Shift in species composition in the Anopheles gambiae complex after implementation of long-lasting insecticidal nets in Dielmo, Senegal

International audienceLong-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) are the cornerstones of malaria vector control. However, the effectiveness of these control tools depends on vector ecology and behaviour, which also largely determine the efficacy of certain Anopheles mosquitoes (Diptera: Culicidae) as vectors. Malaria vectors in sub-Saharan Africa are primarily species of the Anopheles gambiae complex, which present intraspecific differences in behaviour that affect how they respond to vector control tools. The focus of this study is the change in species composition in the An. gambiae complex after the implementation of LLINs in Dielmo, Senegal. The main findings referred to dramatic decreases in the proportions of Anopheles coluzzii and An. gambiae after the introduction of LLINs, and an increase in the proportion of Anopheles arabiensis. Two years after LLINs were first introduced, An. arabiensis remained the most prevalent species and An. gambiae had begun to rebound. This indicated a need to develop additional vector control tools that can target the full range of malaria vectors

Anopheles arabiensis and Anopheles funestus biting patterns in Dielmo, an area of low level exposure to malaria vectors

Background In Dielmo, Senegal, the widespread use of long-lasting insecticidal nets has decreased both the incidence of malaria and the density of theAnophelespopulation. However, persistent low-level malaria transmission may hamper efforts to eliminate the disease. Therefore, continuous monitoring of the vector population is needed in order to improve knowledge ofAnophelesbiting behaviour and to readjust control interventions. Methods In 2015,Anopheleswere collected every month for a whole year and each specimen was identified using morphological and molecular techniques. The biting pattern of each species was analysed according to night (7 pm-7am) and morning (7am-11am) periods, the place of biting and the season. The ELISA CSP technique was used to assess thePlasmodium falciparumsporozoite rate to evaluate the entomological inoculation rate (EIR). Results Anopheles arabiensisandAnopheles funestussensu stricto were found to be the main vectors biting humans. Overall, the biting rate was low, at 3.84bites per night (bpn) and 1.27 bites per morning (bpm), respectively (IRR = 3.04, CI [1.84-5.00], p < 0.001). The EIR was 2.51 and 5.03 infectious bites per year during the night and morning, respectively. During the night, theAn. arabiensisandAn. funestusbiting rate was 1.81 bpn and 1.71 bpn, respectively (IRR = 0.95, CI [0.46-1.92], p = 0.88). During the morning, their density decreased to 0.51 bpm and 0.73 bpm forAn. arabiensisandAn. funestus, respectively (IRR = 1.47, CI [0.58-3.71], p = 0.41). During the night and the morning, no specific trend of indoor or outdoor biting was observed in the dry and rainy season for both vectors. Conclusion This study highlighted low levelAnophelesnocturnal and diurnal biting and the associated risk of malaria transmission. It showed also the influence of the season on the indoor and outdoor biting pattern, indicating that the human population could be exposed all year round to a low level ofAnophelesbites. Control programmes should increase awareness of the use of bed nets throughout the year and promote the development and implementation of complimentary tools to targetAnophelesbiting shortly after dawn when people are still indoors and outside the bed nets

Campagne Rapanui (et transit Manrap): Les frontieres et les deformations de la microplaque Rapanui

The Rapanui Expedition consisted of three legs. The first leg (named Manrap) was a transit between Manzanillo, Mexico and Easter Island, Chile, the second leg (Rapanui 1) consisted of work on the Rapanui (Easter) microplate and was the primary workload of the expedition, and the third leg (Rapanui 2) was a transit from Easter Island, Chile to Tahiti, French Polynesia, and realized additional work on the Rapanui Microplate, and a fuel stop at Hao, French Polynesia. Instrumentation on all legs consisted of standard geophysical tools used on the N/O Jean Charcot

Acquisition of antibodies to variant antigens on the surface of Plasmodium falciparum-infected erythrocytes during pregnancy

Pregnancy-associated malaria is characterized by Plasmodium falciparum adherence to chondroitin sulfate A (CSA) in placenta, through a particular variant surface antigen (VSA). VSA(CSA)-specific IgG are involved in protection against placental malaria. In order to assess the relationship between VSA(CSA)-specific antibody responses and parity as well as protection against placental malaria, the occurrence of P. falciparum infection was assessed in 306 pregnant women from a low malaria transmission area of Senegal. Anti-VSA(CSA) antibodies against three placental parasite isolates were measured by flow cytometry, at enrollment and delivery. Placental infection prevalence rates were highest in primigravidae, but no clear decreasing trend was observed from the second pregnancy onwards. Anti-VSA(CSA) antibody prevalence rates increased with parity. Both anti-VSA(CSA) antibody prevalence rates and levels increased during pregnancy only in women infected with P. falciparum. Although a single or a very limited number of P. falciparum infections were able to induce an anti-VSA(CSA) antibody response, the level or the quality of this response did not appear to confer protection against placental malaria infection. (c) 2006 Elsevier B.V. All rights reserved

Pito and Orongo Fracture Zones: the Northern and Southern Boundaries of the Easter Microplate (southeast Pacific)

The Easter (Rapanui) microplate is a case example of a large dual spreading center system in a region where the fastest seafloor spreading on Earth is occurring today. Recent theoretical models of the tectonic evolution of dual spreading center systems have explored the effects of shear and rigid rotation on the boundaries and internal structure of microplates but the models must be critically constrained by improved relative motion and structural fabric data sets. During the January 1987 Rapanui expedition on the N/O “Jean Charcot” we conducted a Sea Beam/magnetics/ gravity survey of a portion of the microplate boundaries. The method that was used was to fully map selected portions of the boundaries in order to establish precise structural relationships. The northern terminus of the East Rift or eastern boundary of the microplate is expressed as a series of parallel NW-SE trending valleys including what appears to be, with 5890 m depth, the deepest active rift axis mapped in the Pacific today (Pito Rift). The northern end of the Pito Rift merges with an E-W to 083° narrow band of linear faults interpreted to be a transform fault between the Nazca and Easter (Rapanui) plates. The northern triple junction between the Easter (Rapanui), Nazca and Pacific plates is a RFF type with the two transform faults colinear along an approximately E-W direction. The southwestern boundary of the Easter (Rapanui) microplate is marked by a series of en-echelon offsets, outlined by depressions, which merge into an approximately E-W zone where shear must be predominant. The southern triple junction is a RRF junction with an overlapping ridge system. The structural data acquired during the survey provide strong constraints for kinematic models of the microplate. The structural data need to be combined with crustal age determinations in order to derive a model for the evolution of the microplate

Contemporary variations of immune responsiveness during range expansion of two invasive rodents in Senegal

Biological invasions provide unique opportunities for studying life history trait changes over contemporary time scales. As spatial spread may be related to changes in parasite communities, several hypotheses (such as the evolution of increased competitive ability (EICA) or EICA-refined hypotheses) suggest immune changes in invasive species along invasion gradients. Although native hosts may be subject to similar changes in parasite selection pressures, their immune responses have been rarely investigated in invasion contexts. In this study, we evaluated immune variations for invasive house mice Mus musculus domesticus, invasive black rats Rattus rattus and native rodents Mastomys erythroleucus and Mastomys natalensis along well-characterised invasion gradients in Senegal. We focused on antibody-mediated (natural antibodies and complement) and inflammatory (haptoglobin) responses. One invasion route was considered for each invasive species, and environmental conditions were recorded. Natural-antibody mediated responses increased between sites of long-established invasion and recently invaded sites only in house mice. Both invasive species exhibited higher inflammatory responses at the invasion front than in sites of long-established invasion. The immune responses of native species did not change with the presence of invasive species. These patterns of immune variations do not support the EICA and EICA refined hypotheses, and they rather suggest a higher risk of exposure to parasites on the invasion front. Altogether, these results provide a first basis to further assess the role of immune changes in invasion success