49 research outputs found
Transcriptome Profiling of Wild Arachis from Water-Limited Environments Uncovers Drought Tolerance Candidate Genes
ChemInform Abstract: CONFORMATIONAL STUDY OF METHYL ETHERS OF THE TYPE MEOCH2R: AN NMR AND VIBRATIONAL SPECTROSCOPIC STUDY
Influence of Magnetic Susceptibility on Position and Line-width of Proton Magnetic Resonance Lines in Powder Samples of Transition Metal Hydrides
Gamma-tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis
Gamma-tubulin is required for microtubule (MT) nucleation at MT organizing centers such as centrosomes or spindle pole bodies, but little is known about its noncentrosomal functions. We conditionally downregulated gamma-tubulin by inducible expression of RNA interference (RNAi) constructs in Arabidopsis thaliana. Almost complete RNAi depletion of gamma-tubulin led to the absence of MTs and was lethal at the cotyledon stage. After induction of RNAi expression, gamma-tubulin was gradually depleted from both cytoplasmic and microsomal fractions. In RNAi plants with partial loss of gamma-tubulin, MT recovery after drug-induced depolymerization was impaired. Similarly, immunodepletion of gamma-tubulin from Arabidopsis extracts severely compromised in vitro polymerization of MTs. Reduction of gamma-tubulin protein levels led to randomization and bundling of cortical MTs. This finding indicates that MT-bound gamma-tubulin is part of a cortical template guiding the microtubular network and is essential for MT nucleation. Furthermore, we found that cells with decreased levels of gamma-tubulin could progress through mitosis, but cytokinesis was strongly affected. Stepwise diminution of gamma-tubulin allowed us to reveal roles for MT nucleation in plant development, such as organization of cell files, anisotropic and polar tip growth, and stomatal patterning. Some of these functions of gamma-tubulin might be independent of MT nucleation
A Conformational Analysis of 2-(X), 4-(Y)-Substituted Pentanes (X, Y = OH, SH), Using Chiroptical Properties and 1H NMR Spectroscopy
Properties of Bioriented Nylon 6 Films related to film production technology
none7F. Bianchi; S. Cantagallo; G. Consolati; M. Laporta; M. Pegoraro; G. Tieghi; L. ZanderighiBianchi, Fiorenzo; S., Cantagallo; Consolati, Giovanni; M., Laporta; M., Pegoraro; Tieghi, Giuseppe; L., Zanderigh