Detecting insider threat within institutions using CERT dataset and different ML techniques

The reason of countries development in industrial and commercial enterprises fields in those countries. The security of a particular country depends on its security institutions, the confidentiality of its employees, their information, the target's information, and information about the forensic evidence for those targets. One of the most important and critical problems in such institutions is the problem of discovering an insider threat that causes loss, damage, or theft the information to hostile or competing parties. This threat is represented by a person who represents one of the employees of the institution, the goal of that person is to steal information or destroy it for the benefit of another institution's desires. The difficulty in detecting this type of threat is due to the difficulty of analyzing the behavior of people within the organization according to their physiological characteristics. In this research, CERT dataset that produced by the University of Carnegie Mellon University has been used in this investigation to detect insider threat. The dataset has been preprocessed. Five effective features were selected to apply three ML techniques Random Forest, Naïve Bayes, and 1 Nearest Neighbor. The results obtained and listed sequentially as 89.75917519%, 91.96650826%, and 94.68205476% with an error rate of 10.24082481%, 8.03349174%, and 5.317945236%

Marion County Newspapers

The Florida Press Association, in celebrating the 75th anniversary of that organization, asked me to prepare a history of the Ocala Star-Banner. The article prepared at that time has been revised somewhat and brought up to date

Analytical determination of copper products

It is a well known fact that the presence of impurities in copper products has been a source of annoyance to the Chemist. The object of this thesis was to make myself familiar with the actions of different impurities in copper products, and the methods for the determination of Arsenic in the same --page 1

Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease

The in vitro study of the pathogenesis of inflammatory bowel disease (IBD) requires a cell model which closely reflects the characteristics of the in vivo intestinal epithelium. This study aimed to investigate the application of L-pNIPAM hydrogel as a scaffold to develop a long-term 3D co-culture model of Caco-2 and HT29-MTX cells under conditions analogous to inflammation, to determine its potential use in studying IBD. Monocultures and co-cultures were layered on L-pNIPAM hydrogel scaffolds and maintained under dynamic culture conditions for up to 12 weeks. Treatments with IL-1β, TNFα, and hypoxia for 1 week were used to create an inflammatory environment. Following prolonged culture, the metabolic activity of Caco-2 monoculture and 90% Caco-2/10% HT29-MTX co-cultures on L-pNIPAM hydrogels were increased, and finger-like structures, similar in appearance to villi were observed. Following treatment with IL-1β, TNFα and hypoxia, ALP and ZO-1 were decreased, MUC2 increased, and MUC5AC remained unchanged. ADAMTS1 was increased in response to hypoxia. Caspase 3 expression was increased in response to TNFα and hypoxic conditions. In conclusion, L-pNIPAM hydrogel supported long-term co-culture within a 3D model. Furthermore, stimulation with factors seen during inflammation recapitulated features seen during IBD

Developing models of the small intestine

Inflammatory bowel disease (IBD) is a chronic autoimmune disease characterised by inflammation of the gastrointestinal tract. The pathogenesis of IBD is not fully understood and curative therapies are lacking. Consequently, development of robust intestine models, representative of the pathogenesis of IBD remains an unmet need. Thus, the overall aims of the studies presented in this thesis were to develop a number of models of small intestine including: genetically engineered murine model, epithelial cell culture models, and an intestinal stem cell organoid model which could reflect or be used to study the pathogenesis of IBD. Interleukin 1 (IL-1) is an important mediator of inflammation and tissue damage in IBD. The balance between IL-1 and IL-1Ra as a natural inhibitor plays a vital role in a variety of diseases. Here, this thesis investigated whether changes seen during IBD could be induced spontaneously by the removal of IL-1Ra in mice that lack a functional IL-1rn gene. Data presented from this thesis highlighted the importance of IL-1 in the pathogenesis of inflammatory bowel disease. In addition, the potential of L-pNIPAM hydrogel scaffolds, which were developed by the research team at Sheffield Hallam University, was utilised to develop long-term 3D co-cultures of layered Caco-2 and HT29-MTX cells under conditions representative of inflammation by treatment with IL-1β, TNFα, and hypoxia (1% O2) for 1 week was investigated. In vitro cell culture studies in this thesis have demonstrated that L-pNIPAM hydrogel supported long-term 3D co-culture model and stimulation with factors seen during inflammation recapitulated features of IBD. Finally, the potential of L-pNIPAM hydrogel scaffolds to develop 3D intestinal stem cell organoid model was investigated. The in vitro study demonstrated the ability of L-pNIPAM hydrogel as scaffold to support organoid formation and cell differentiation in vitro from small intestinal crypts and Lgr5+ stem cells isolated from mice

Tissue engineering laboratory models of the small intestine.

In recent years, three-dimensional (3D) cell culture models of the small intestine have gained much attention. These models support cell proliferation, migration, and differentiation, and encourage tissue organization which is not possible in two-dimensional (2D) culture systems. Furthermore, the use of a wide variety of cell culture scaffolds and support substrates have revealed considerable differences in cell behavior and tissue organization. These systems have been used in combination with intestinal stem cells, organoid units or human colonic adenocarcinoma cell lines such as Caco-2 and HT29-MTX to generate a number of in vitro and in vivo models of the intestine. Here, we review the current 2D and 3D tissue engineering models of the intestine to determine the most effective sources of intestinal cells and current research on support scaffolds capable of inducing the morphological architecture and function of the intestinal mucosa