Dufilho, Grandchamps, or Peyroux? The Development of Professional Pharmacy in Colonial and Early National American Louisiana

This paper will examine the hidden history of the New Orleans Pharmacy Museum and investigate the claims regarding the first licensed pharmacist in the United States. Drawing from legislative, church, medical, legal and institutional records, this study argues that colonial control, such as regulations governing medical practice and licensing requirements, established by continental powers in their overseas colonies, tended to recreate traditions and laws found in the home countries. For instance, the more rigorous licensing requirements for medical professionals, as practiced in France and Spain, were also the custom in Louisiana. However, when Louisiana became part of the Unites States, these regulations were relaxed, reflecting the laissez-faire policy of English laws and custom. This work challenges the bias often found in the presentation of American historical experience that makes claims for English colonial traditions informing the entire American experience

Managing Soybean Rustin South Dakota in 2006

Soybean rust, caused by the fungus Phakopsora pachyrhizi, was first introduced to the U.S. in Fall 2004. It survived the winter in small pockets of infected kudzu in Florida and produced a widespread low-severity disease in the southeastern U.S. in 2005. During Winter 2005, the pathogen survived over a much wider area and appears to have become established in Mexico. That means the threat from soybean rust is greater in 2006 than in 2005, and we can expect that the threat will generally increase over the coming years as the pathogen becomes established in new areas with little risk of winter freeze. Fungicides remain the only practical and effective control for this disease. Several fungicides have received EPA labeling and more products are being considered. Which fungicide products are available is a constantly changing landscape. Check with your local county Extension office for a listing of the most current products or go to http://plantsci.sdstate.edu/planthealth and link to Soybean Rust

Comparison of three microsatellite analysis methods for detecting genetic diversity in Phytophthora sojae (Stramenopila: Oomycete)

Analysis of an organism’s genetic diversity requires a method that gives reliable, reproducible results. Microsatellites are robust markers, however, detection of allele sizes can be difficult with some systems as well as consistency among laboratories. In this study, our two laboratories used 219 isolates of Phytophthora sojae to compare three microsatellite methods. Two capillary electrophoresis methods, the Applied Biosystems 3730 Genetic Analyzer and the CEQ 8000 Genetic Analysis system, detected an average of 2.4-fold more alleles compared to gel electrophoresis with a mean of 8.8 and 3.6 alleles per locus using capillary and gel methods, respectively. The two capillary methods were comparable, although allele sizes differed consistently by an average of 3.2 bp across isolates. Differences between capillary methods could be overcome if reference standard DNA genotypes are shared between collaborating laboratories

Pathotype Diversity of Phytophthora sojae in Eleven States in the United States

Pathotype diversity of Phytophthora sojae was assessed in 11 states in the United States during 2012 and 2013. Isolates of P. sojae were recovered from 202 fields, either from soil samples using a soybean seedling bioassay or by isolation from symptomatic plants. Each isolate was inoculated directly onto 12 soybean differentials; no Rps gene or Rps 1a, 1b, 1c, 1k, 3a, 3b, 3c, 4, 6, 7, or 8. There were 213 unique virulence pathotypes identified among the 873 isolates collected. None of the Rps genes were effective against all the isolates collected but Rps6 and Rps8 were effective against the majority of isolates collected in the northern regions of the sampled area. Virulence toward Rps1a, 1b, 1c, and 1k ranged from 36 to 100% of isolates collected in each state, while virulence to Rps6 and Rps8 was less than 36 and 10%, respectively. Depending on the state, the effectiveness of Rps3a ranged from totally effective to susceptible to more than 40% of the isolates. Pathotype complexity has increased in populations of P. sojae in the United States, emphasizing the increasing importance of stacked Rps genes in combination with high partial resistance as a means of limiting losses to P. sojae

Integrated Management Strategies for Phytophthora sojae Combining Host Resistance and Seed Treatments

Phytophthora sojae has re-emerged as a serious soybean pathogen in the past decade. This may be due in part to changes in resistance levels in current cultivars, adoption of P. sojae populations to deployed Rps genes, and highly favorable environments in the past decade. This multilocation study evaluated the effect of seed treatments on the incidence and severity of Phytophthora root and stem rot on soybeans with different combinations of Rps genes and levels of partial resistance. The efficacy of the seed treatments was highly variable across locations. Seed treatments (metalaxyl and mefenoxam) provided protection and increased yields across cultivars in locations where rain or irrigation occurred shortly after planting (Ohio, South Dakota, and Ontario). However, there were no significant differences in stand or yield consistently across cultivars in Iowa, Nebraska, Wisconsin, or Ohio, where heavy precipitation did not occur until later growth stages. The environment, levels of inoculum, and pathogen complex may have played a role in the different responses to the seed treatments and to the different combinations of Rps genes and levels of partial resistance to P. sojae in the cultivars. Fields that are poorly drained and have P. sojae populations with complex pathotypes may benefit the most from seed treatments. Individual fields where producers may see the greatest benefit to utilizing these integrated management strategies will need to be identified

Phenotypic Characterization of a Major Quantitative Disease Resistance Locus for Partial Resistance to Phytophthora sojae

Major quantitative disease resistance loci (QDRLs) are rare in the Phytophthora sojae (Kaufmann and Gerdemann)–soybean [Glycine max (L). Merr.] pathosystem. A major QDRL on chromosome 18 (QDRL-18) was identified in PI 427105B and PI 427106. QDRL-18 represents a valuable resistance source for breeding programs. Thus, our objectives were to determine its isolate specificity and measure its effect on yield and resistance to both P. sojae and other soybean pathogens. We characterized near isogenic lines (NILs) developed from F7 recombinant inbred lines heterozygous at QDRL-18; NILs represent introgressions from PI 427105B, PI 427106, and susceptible ‘OX20- 8’. The introgressions from PI 427105B and PI 427106 increased resistance to P. sojae by 11 to 20% and 35 to 40%, respectively, based on laboratory and greenhouse assays, and increased yield by 13 to 29% under disease conditions. The resistant introgression from PI 427105B was also effective against seven P. sojae isolates with no isolate specificity detected. Based on quantitative polymerase chain reaction assays, NILs with the susceptible introgression had significantly higher relative levels of P. sojae colonization 48 h after inoculation. No pleiotropic effects for resistance to either soybean cyst nematode or Fusarium graminearum were detected. This information improves soybean breeders’ ability to make informed decisions regarding the deployment of QDRL-18 in their respective breeding programs

An Immunofluorescence Assay to Detect Urediniospores of \u3ci\u3ePhakopsora pachyrhizi\u3c/i\u3e

An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact nongerminated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006