Surface Treatment of Metals

The purpose or this thesis is to relate to others my experimentation of various types of metals, and the methods employed to decorate the surfaces of these particular metals. The differences, and similarities in means of working with and decorating each type of metal used is also discussed. The first part of the paper is devoted to a brief history of metal techniques. This gives a better understanding as to when the particular techniques were first employed and how many of them were almost lost with the arrival of nineteeth century industralization. Techniques which I used, and the various means of achieving them is discussed next. This brings an understanding to the reader when the series or works are explained in the final section of the tesis. The final portion of the paper gives detailed explainations of each of the pieces done while experimenting with the various techniques. Particular Problems which arose with the incorporation of techniques are discussed, along with the sucess of others. I conclude the paper by noting that the major problem in my working with surface treatments was, that more forethought about accual construction was necessary. New techniques needed to be explored to avoid the quailities of dullness and staliness

Surface Treatment of Metals

The purpose or this thesis is to relate to others my experimentation of various types of metals, and the methods employed to decorate the surfaces of these particular metals. The differences, and similarities in means of working with and decorating each type of metal used is also discussed. The first part of the paper is devoted to a brief history of metal techniques. This gives a better understanding as to when the particular techniques were first employed and how many of them were almost lost with the arrival of nineteeth century industralization. Techniques which I used, and the various means of achieving them is discussed next. This brings an understanding to the reader when the series or works are explained in the final section of the tesis. The final portion of the paper gives detailed explainations of each of the pieces done while experimenting with the various techniques. Particular Problems which arose with the incorporation of techniques are discussed, along with the sucess of others. I conclude the paper by noting that the major problem in my working with surface treatments was, that more forethought about accual construction was necessary. New techniques needed to be explored to avoid the quailities of dullness and staliness

Ino80 is essential for proximal-distal axis asymmetry in part by regulating Bmp4 expression

Western blotting confirming custom Ino80 antibody. Western analysis of Ino80 protein expression in wild-type and Ino80 KO ESCs using custom antibody VCU23 (a) and a commercially available Ino80 antibody (ProteinTech Cat# 18810-1-AP) (b). In each case Ponceau S was used as a loading control. (TIF 6874 kb

Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes

Amutation in the small GTPase Rab38 gives rise to the mouse coat color phenotype “chocolate” (cht), implicating Rab38 in the regulation of melanogenesis. However, its role remains poorly characterized. We report that cht Rab38G19V is inactive and that the nearly normal pigmentation in cht melanocytes results from functional compensation by the closely related Rab32. In cht cells treated with Rab32-specific small interfering RNA, a dramatic loss of pigmentation is observed. In addition to mature melanosomes, Rab38 and Rab32 localize to perinuclear vesicles carrying tyrosinase and tyrosinase-related protein 1, consistent with a role in the intracellular sorting of these proteins. In Rab38/Rab32-deficient cells, tyrosinase appears to be mistargeted and degraded after exit from the trans-Golgi network (TGN). This suggests that Rab38 and Rab32 regulate a critical step in the trafficking of melanogenic enzymes, in particular, tyrosinase, from the TGN to melanosomes. This work identifies a key role for the Rab38/Rab32 subfamily of Rab proteins in the biogenesis of melanosomes and potentially other lysosome-related organelles

Novel and Known Protein Tyrosine Kinases and Their Abnormal Expression in Human Melanoma

We have used the polymerase chain reaction and Northern blotting to identify protein tyrosine kinases that may play an an important role in the process of melanoma initiation and progression. Degenerate primers from the conserved catalytic domain of tyrosine kinase genes were used to amplify and clone partial cDNA sequences from a human melanoma cell line (DX3-LT5.1) and normal human melanocytes. When the melanoma reaction products were sequenced, 13 distinct clones were found, of which one is novel to date and has provisionally been named MEK (for melanocytic kinase). Of the remaining 12 known kinases, only two, ERB-B2 and IGFI-1-R, have previously been reported in pigment cells. Reaction products from melanocytes included only eight of these 13 sequences. To test for quantitative differences in tyrosine kinase expression between normal and malignant cells, a panel of eight melanoma lines and normal melanocytes was analyzed by Northern blotting. Two tyrosine kinases (JTK-14/TIE and TYRO-9) were detected in some melanomas but were not found in normal melanocytes, whereas others, including MEK, appeared to be overexpressed in some malignant lines. A minority of kinases showed either no change or a reduction in the level of mRNA. Expression of tyrosine kinases varied independently, and individual lines contained various combinations of these enzymes. Our findings are consistent with an increased overall expression of these putative growth factor receptors during melanoma development

The mediating effect of task presentation on collaboration and children's acquisition of scientific reasoning

There has been considerable research concerning peer interaction and the acquisition of children's scientific reasoning. This study investigated differences in collaborative activity between pairs of children working around a computer with pairs of children working with physical apparatus and related any differences to the development of children's scientific reasoning. Children aged between 9 and 10 years old (48 boys and 48 girls) were placed into either same ability or mixed ability pairs according to their individual, pre-test performance on a scientific reasoning task. These pairs then worked on either a computer version or a physical version of Inhelder and Piaget's (1958) chemical combination task. Type of presentation was found to mediate the nature and type of collaborative activity. The mixed-ability pairs working around the computer talked proportionally more about the task and management of the task; had proportionally more transactive discussions and used the record more productively than children working with the physical apparatus. Type of presentation was also found to mediated children's learning. Children in same ability pairs who worked with the physical apparatus improved significantly more than same ability pairs who worked around the computer. These findings were partially predicted from a socio-cultural theory and show the importance of tools for mediating collaborative activity and collaborative learning

Victim evaluations of face-to-face restorative justice conferences: A quasi-experimental analysis

One major goal of face-to-face restorative justice (RJ) is to help heal the psychological harm suffered by crime victims (Braithwaite, 2002). Substantial evidence from randomized controlled trials (RCTs) has shown that this can be accomplished (Strang, 2002) and more trials are underway (Sherman & Strang, 2004). These outcomes are even more clearly, if less rigorously, demonstrated through retrospective interviews of victims about their feelings before and after RJ took place. We review the responses of victims (N = 210) who participated in trials in Canberra (Australia) and in London, Thames Valley, and Northumbria (UK). Despite substantial variations in offense types, social contexts, nation and race, before-after changes revealed by qualitative and quantitative data are all in the same beneficial direction

Flamingo Vol. II N 2

Keeler, Clyde. Dad\u27s Day . Picture. 0. Puppet. Untitled. Prose. 1. Crescent. Untitled. Prose. 1. Williams, Charles L. As To Reading Biography . Prose. 5. Verlaine, Paul. Kyrie Eleison . Poem. 6. Anonymous. Mystery . Poem. 6. R. To You . Poem. 7. Q. The Passion Flower . Poem. 7. Bennett, G.W. November . Poem. 7. R. Storm . Poem. 7. Anonymous. Untitled.Prose. 8. Anonymous. No Doubt! . Poem. 8. Anonymous. Untitled. Picture. 8. McCutcheon, Dorothy. Ready-Made Reputations . Prose. 9. Anonymous. Untitled. Prose. 9. Anonymous. Untitled. Prose. 10. Ubersax, Delmar. Untitled. Prose. 10. Anonymous. Statistics From The Football Centers . Prose. 10. Anonymous. Our Own Handbook . Prose. 11. Anonymous. Real Reels . Prose. 12. Anonymous. Tire Trouble at The Round Table . Prose. 12. Anonymous. Untitled. Prose. 14. Anonymous. The Return From Dayton . Cartoon. 16. Anonymous. Untitled. Prose. 18. Anonymous. Down The Street . Poem. 18. Anonymous. The 7:30 . Poem. 18. Lord Jeff. In The Hole . Poem. 18. Anonymous. How It\u27s Done . Prose. 19. Anonymous. A Zoological Dream . Picture. 19. Anonymous. A Smooth Line . Prose. 20. Anonymous. Untitled. Prose. 20. Anonymous. You\u27ve Heart it . Prose. 20. Anonymous. Dog Gone! . Prose. 21. Anonymous. Untitled. Prose. 21. Cols Dis. Untitled. Poem. 21. Anonymous. Eddie Tor\u27s Lament . Poem. 22. Anonymous. Untitled. Prose. 22. Anonymous. The Long And Short of it. Prose. 22. Anonymous. Final Exam. In Zetetic Jactation 613 (You Look it up.) . Prose. 24. Pope of Fools. Love Letters of a Sceptic . Prose. 25. Anonymous. Untitled. Prose. 26. Williams, Grace. Untitled. Picture. 28. Anonymous. Untitled. Prose. 28. Goblin. It is a Wise Landlady . Prose. 28. Anonymous. Untitled. Prose. 28. Drexard. Untitled. Prose. 29. Chaparral. Untitled. Prose. 29. Punch Bowl. The Trifler . Prose. 29. Anonymous. His Finger Got Stuck in the Bowling Ball . Picture. 30. Chaparral. Untitled. Prose. 30. Banter. Untitled. Prose. 30. Tiger. Untitled. Prose. 30. Brown Jug. Untitled. Prose. 31. Octopus. Untitled. Prose. 31. Burr. Untitled. Prose. 31. Squib. Untitled. Prose. 31. Ubersax, Delmar. Untitled. Picture. 32. Purple Cow. Untitled. Prose. 32. Mather, William G. A Ditty . Poem. 12

Functional Characterization and Atomic Force Microscopy of a DNA Repair Protein Conjugated to a Quantum Dot

Quantum dots (QDs) possess highly desirable optical properties that make them ideal fluorescent labels for studying the dynamic behavior of proteins. However, a lack of characterization methods for reliably determining protein–quantum dot conjugate stoichiometry and functionality has impeded their widespread use in single-molecule studies. We used atomic force microscopic (AFM) imaging to demonstrate the 1:1 formation of UvrB–QD conjugates based on an antibody-sandwich method. We show that an agarose gel-based electrophoresis mobility shift assay and AFM can be used to evaluate the DNA binding function of UvrB–QD conjugates. Importantly, we demonstrate that quantum dots can serve as a molecular marker to unambiguously identify the presence of a labeled protein in AFM images