Differences in Albizia odoratissima genetic diversity between Hainan Island and mainland populations in China

BackgroundThis study aimed at exploring unique population genetic characteristics of Albizia odoratissima (Linn. f) Benth on Hainan Island to provide a scientific basis for its rational utilization and protection.MethodsIt analyzed the genetic diversity and structure of 280 individuals from 10 subpopulations of A. odoratissima from Hainan Island and Baise City using 16 expression sequence markers - simple sequence repeat markers.ResultsThe genetic diversity of Hainan population (I = 0.7290, He = 0.4483) was lower than that of the Baise population (I = 0.8722, He = 0.5121). Compared with the Baise population (Nm = 2.0709, FST = 0.1077), the Hainan Island population (Nm = 1.7519, FST = 0.1249) exhibited lower gene flow and higher degree of genetic differentiation. Molecular variance and genetic differentiation analyses showed that the main variation originated from individuals within the subpopulation. There were significant differences in the genetic structure between Hainan and Baise populations. It grouped according to geographical distance, consistent with the Mantel test results (R2 = 0.77, p = 0.001). In conclusion, the genetic diversity of the island A. odoratissima population was lower than that distributed on land, the two populations exhibited obvious genetic structure differences. Both the degrees of inbreeding and genetic differentiation were higher in the island population than in the land population

Study on roof deformation mechanism and control technology of cross-fault roof cutting and pressure relief self-forming roadway

Aiming at the problem that roof deformation of cross fault roof cutting and pressure relief self-formed roadway is large and difficult to support, the mechanism of roof deformation of self-formed roadway in cross fault roof cutting and pressure-relieving roadway is studied based on the engineering background of the transport channeling of No. 11101 face in the eastern district of Qipanjing Coal Mine, and the effect of the support method of “roof cutting and pressure relief + constant resistance anchor cable” on roof deformation control of self-formed roadway in cross-fault roof cutting and pressure relieving roadway is studied. A mechanical analysis model was established to study the influence of the relevant parameters of the roof on the direct roof stress of the roadway, and the deformation process of the roof of the roadway was divided into four stages. The calculation method of each stage and the total vertical displacement of the roof of the roadway were studied and the calculation formula was given, and the relevant parameters were substituted into the formula to solve the vertical displacement of the roof of the roadway across the fault. 3DEC numerical simulation software was used to establish the numerical calculation model of self-formed roadway with cross fault roof cutting and pressure relief, and the stress-strain evolution law of roadway roof near the fault and the control effect of constant resistance anchor cable were studied. The research results show that the error between the numerical simulation and theoretical analysis data of roadway roof deformation is 1.14% and 4.04%, respectively. The constant resistance anchor cable can effectively reduce the deformation of roadway roof. Compared with the non-constant resistance anchor cable model, the vertical displacement of roof on slit side of upper wall roadway is reduced to 16.8%, and the vertical displacement of roof on slit side of lower wall roadway is reduced to 50.7%. The roof of upper and lower wall roadway will have different degree of stress concentration in the process of mining through the measurement section of roadway, and the vertical stress concentration value of upper wall roadway roof is larger than that of lower wall roadway, which is 5.72 MPa and 4.48 MPa respectively. The constant resistance anchor cable can reduce the deformation rate of roadway roof near the fault, and control the deformation of roadway roof jointly with the gravel wall after the filling is completed. By comparing the displacement monitoring data of surrounding rock along the channel of No. 11101 face transport with the calculation results of the theoretical model, the error is less than 10%, which proves that the support method of “roof cutting and pressure relief + constant resistance anchor cable” has a good control effect on the roof deformation of cross fault roadway

Use of the 2A Peptide for Generation of Multi-Transgenic Pigs through a Single Round of Nuclear Transfer

Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation. Most multi-transgenic pigs have been produced by complex and time-consuming breeding programs using multiple single-transgenic pigs. This study explored the feasibility of producing multi-transgenic pigs using the viral 2A peptide in the light of previous research indicating that it can be utilized for multi-gene transfer in gene therapy and somatic cell reprogramming. A 2A peptide-based double-promoter expression vector that mediated the expression of four fluorescent proteins was constructed and transfected into primary porcine fetal fibroblasts. Cell colonies (54.3%) formed under G418 selection co-expressed the four fluorescent proteins at uniformly high levels. The reconstructed embryos, which were obtained by somatic cell nuclear transfer and confirmed to express the four fluorescent proteins evenly, were transplanted into seven recipient gilts. Eleven piglets were delivered by two gilts, and seven of them co-expressed the four fluorescent proteins at equivalently high levels in various tissues. The fluorescence intensities were directly observed at the nose, hoof and tongue using goggles. The results suggest that the strategy of combining the 2A peptide and double promoters efficiently mediates the co-expression of the four fluorescent proteins in pigs and is hence a promising methodology to generate multi-transgenic pigs by a single nuclear transfer

Immunodeficient Rabbit Models: History, Current Status and Future Perspectives

Production of immunodeficient (ID) models in non-murine animal species had been extremely challenging until the advent of gene-editing tools: first zinc finger nuclease (ZFN), then transcription activator-like effector nuclease (TALEN), and most recently clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR)/Cas9. We and others used those gene-editing tools to develop ID rabbits carrying a loss of function mutation in essential immune genes, such as forkhead box protein N1 (FOXN1), recombination activating gene 1/2 (RAG1/2), and interleukin 2 receptor subunit gamma (IL2RG). Like their mouse counterparts, ID rabbits have profound defects in their immune system and are prone to bacterial and pneumocystis infections without prophylactic antibiotics. In addition to their use as preclinical models for primary immunodeficient diseases, ID rabbits are expected to contribute significantly to regenerative medicine and cancer research, where they serve as recipients for allo- and xeno-grafts, with notable advantages over mouse models, including a longer lifespan and a much larger body size. Here we provide a concise review of the history and current status of the development of ID rabbits, as well as future perspectives of this new member in the animal model family

A taxonomy of web prediction algorithms

Web prefetching techniques are an attractive solution to reduce the user-perceived latency. These techniques are driven by a prediction engine or algorithm that guesses following actions of web users. A large amount of prediction algorithms has been proposed since the first prefetching approach was published, although it is only over the last two or three years when they have begun to be successfully implemented in commercial products. These algorithms can be implemented in any element of the web architecture and can use a wide variety of information as input. This affects their structure, data system, computational resources and accuracy. The knowledge of the input information and the understanding of how it can be handled to make predictions can help to improve the design of current prediction engines, and consequently prefetching techniques. This paper analyzes fifty of the most relevant algorithms proposed along 15 years of prefetching research and proposes a taxonomy where the algorithms are classified according to the input data they use. For each group, the main advantages and shortcomings are highlighted. © 2012 Elsevier Ltd. All rights reserved.This work has been partially supported by Spanish Ministry of Science and Innovation under Grant TIN2009-08201, Generalitat Valenciana under Grant GV/2011/002 and Universitat Politecnica de Valencia under Grant PAID-06-10/2424.Domenech, J.; De La Ossa Perez, BA.; Sahuquillo Borrás, J.; Gil Salinas, JA.; Pont Sanjuan, A. (2012). A taxonomy of web prediction algorithms. Expert Systems with Applications. 39(9):8496-8502. https://doi.org/10.1016/j.eswa.2012.01.140S8496850239

Decreased plasma levels of PDGF-BB, VEGF-A, and HIF-2α in preterm infants after ibuprofen treatment

IntroductionIbuprofen is one of the most common non-steroidal anti-inflammatory drugs used to close patent ductus arteriosus (PDA) in preterm infants. PDA is associated with bronchopulmonary dysplasia (BPD), while PDA closure by ibuprofen did not reduce the incidence of BPD or death. Previous studies have indicated an anti-angiogenesis effect of ibuprofen. This study investigated the change of angiogenic factors after ibuprofen treatment in preterm infants.MethodsPreterm infants with hemodynamically significant PDA (hsPDA) were included. After confirmed hsPDA by color doppler ultrasonography within 1 week after birth, infants received oral ibuprofen for three continuous days. Paired plasma before and after the ibuprofen treatment was collected and measured by ELISA to determine the concentrations of platelet-derived growth factor-BB (PDGF-BB) and vascular endothelial growth factor A (VEGF-A), and hypoxia-inducible factor-2α (HIF-2α).Results17 paired plasma from infants with hsPDA were collected. The concentration of PDGF-BB and VEGF-A significantly decreased after ibuprofen treatment (1,908 vs. 442 pg/mL for PDGF-BB, 379 vs. 174 pg/mL for VEGF-A). HIF-2α level showed a tendency to decrease after ibuprofen treatment, although the reduction was not statistically significant (p = 0.077).ConclusionThis study demonstrated decreased vascular growth factors after ibuprofen exposure in hsPDA infants

Effects of sand burial on biomass, chlorophyll fluorescence and extracellular polysaccharides of man-made cyanobacterial crusts under experimental conditions

Soil cyanobacterial crusts occur throughout the world, especially in the semiarid and arid regions. It always encounters sand burial, which is an important feature of mobile sand dunes. A greenhouse 41 study was conducted to determine the effects of sand burial on biomass, chlorophyll fluorescence and extracellular polysaccharides of man-made cyanobacterial crusts in six periods of time (0, 5, 10, 15, 20 and 30 d after burying) and at five depths (0, 0.2, 0.5, 1 and 2cm). The results indicated that with the increase of the burial time and burial depth extracellular polysaccharides content and Fv/Fm decreased correspondingly and there were no significant differences between 20 and 30 burial days under different burial depths. The degradation of chlorophyll a content appeared only at 20 and 30 burial days and there was also no significant difference between them under different burial depths. It was also observed a simultaneous decrease of the values of the Fv/Fm and the content of extracellular polysaccharides happened in the crusted cyanobacterium Microcoleus vaginatus Gom. It may suggest that there exists a relationship between extracellular polysaccharides and recovery of the activity of photosystem II (PS II) after rehydration.Soil cyanobacterial crusts occur throughout the world, especially in the semiarid and arid regions. It always encounters sand burial, which is an important feature of mobile sand dunes. A greenhouse 41 study was conducted to determine the effects of sand burial on biomass, chlorophyll fluorescence and extracellular polysaccharides of man-made cyanobacterial crusts in six periods of time (0, 5, 10, 15, 20 and 30 d after burying) and at five depths (0, 0.2, 0.5, 1 and 2cm). The results indicated that with the increase of the burial time and burial depth extracellular polysaccharides content and Fv/Fm decreased correspondingly and there were no significant differences between 20 and 30 burial days under different burial depths. The degradation of chlorophyll a content appeared only at 20 and 30 burial days and there was also no significant difference between them under different burial depths. It was also observed a simultaneous decrease of the values of the Fv/Fm and the content of extracellular polysaccharides happened in the crusted cyanobacterium Microcoleus vaginatus Gom. It may suggest that there exists a relationship between extracellular polysaccharides and recovery of the activity of photosystem II (PS II) after rehydration

Generation of Rabbit Models by Gene Editing Nucleases

Due to the lack of germline transmitting pluripotent stem cells (PSCs) cell lines and the extreme difficulty of somatic cell nuclear transfer (SCNT) in rabbit, the gene targeting technology in rabbit was lagging far behind those in rodents and in farm animals. As a result, the development and application of genetically engineered rabbit model are much limited. With the advent of gene editing nucleases, including ZFN, TALEN, and CRISPR/Cas9, it is now possible to produce gene targeting (i.e., knockout, knockin) rabbits with high success rates. In this chapter, we describe a comprehensive, step-by-step protocol for rabbit genome editing based on gene editing nucleases with specific emphasis of CRISPR/Cas9.http://deepblue.lib.umich.edu/bitstream/2027.42/174902/2/2019-MMB-Protocol_GenerationOfRabbitModels.pdfPublished versio

LncRNA 148400 Promotes the Apoptosis of Renal Tubular Epithelial Cells in Ischemic AKI by Targeting the miR−10b−3p/GRK4 Axis

Although recent studies have reported that long non-coding RNA (lncRNA) is involved in the development of ischemic acute kidney injury (AKI), the exact function and regulatory mechanism of lncRNAs in ischemic AKI remain largely unknown. Herein, we found that ischemic injury promoted the expression of lncRNA 148400 in mouse proximal tubule-derived cell line (BUMPT) and C57BL/6J mice. Furthermore, the lncRNA148400 mediates ischemic injury-induced apoptosis of BUMPT cells. Mechanistically, lncRNA 148400 sponged miR−10b−3p to promote apoptosis via GRK4 upregulation. Finally, knockdown of lncRNA 148400 alleviated the I/R-induced deterioration of renal function, renal tubular injury, and cell apoptosis. In addition, cleaved caspase−3 is increased via targeting the miR−10b−3p/GRK4 axis. Collectively, these results showed that lncRNA 148400/miR−10b−3p/GRK4 axis mediated the development of ischemic AKI