APPLICATION OF HIGH SPEED PANNING ON THE TECHNICAL ANALYSIS OF POLE VAULT

INTRODUCTION: The pole vault is an event with the characteristics of a complicated technical movement and a large motion space. Generally, its field of view needs a near 10m horizontal shooting scope and an above 6m vertical shooting scope. The factors such as the edge distortion should be also considered. If the fixed photography of 16mm high-speed camera is used, the image formation is too small to read photographic images. Therefore, the high speed panning of a 35mm camera has been used to record the performance of an elite Chinese pole-vault athlete. On the basis of the shooting film of his movement in competitions, a biomechanical analysis and diagnosis have been made in order to put forward constructive suggestions for the coach and athlete to assist with specific training to improve technique

A study of evacuation efficiency of a hopper-shape exit by using mice under high competition

Exit is the bottleneck of an evacuation from a room and the flow rate through an exit is believed to be depended on its width. A series of experiments were conducted in a bi-dimensional container where mice were driven to pass through two kinds of exit of the identical width, i.e., a conventional exit and a hopper-shape exit. The evacuation efficiency of the two exits was experimentally compared by using mice under competition. The results showed that a hopper-shape exit reduces the escape time by 25% compared with a conventional exit. Further study was conducted with the presence of a column in front of the two exits. The presence of a column in front of the conventional exit increases the escape time by 22.5%. On the contrary, the placement of column in front of the hopper-shape exit reduces the escape time by 48%. The study showed that the escape efficiency could be greatly improved by appropriately redesigning configuration of exit

Epigenetic regulation of CD271, a potential cancer stem cell marker associated with chemoresistance and metastatic capacity.

Cancer stem cells (CSCs) are considered to be the cause of tumor initiation, metastasis and recurrence. Additionally, CSCs are responsible for the failure of chemotherapy and radiotherapy. The isolation and identification of CSCs is crucial for facilitating the monitoring, therapy or prevention of cancer. We aimed to identify esophageal squamous cell carcinoma (ESCC) stem-like cells, the epigenetic mechanism and identify novel biomarkers for targeting ESCC CSCs. Sixty-three paired ESCC tissues and adjacent non-cancerous tissues were included in this study. CD271, which was identified as the CSC marker for melanoma, was assessed using quantitative PCR (qPCR). Using flow cytometry, we isolated CD271+ cells comprising 7.5% of cancer cells from the KYSE70 cell line. Sphere formation and anchorage-independent growth were analyzed in CD271+ and CD271- cancer cells, respectively. qPCR was used to detect stem-related genes and CCK-8 was performed to analyze the sensitivity to chemotherapy in the two groups. Bisulfite genomic sequencing was used to analyze the methylation status. CD271 expression was significantly higher in ESCC tissues than in adjacent non-cancerous tissues. Compared with CD271- cancer cells, CD271+ cancer cells showed a higher ability of sphere and colony formation, a high level expression of stem-related gene, and resistance to chemotherapy. The expression of CD271 was induced by a demethylation agent. In conclusion, CD271+ ESCC cells possess stem-like properties. CD271 can potentially act as a prognostic marker for ESCC, whose expression is regulated epigenetically

Weight of Evidence Method and Its Applications and Development

AbstractThe development and applications about the weight of evidence technology in recent years are reviewed. This paper introduced the improved weight of evidence in remote sensing image processing and in different fields of application. Summary its constraints and existent problems. Look forward to the weight of evidence for the practical application

Sevoflurane ameliorates doxorubicin-induced myocardial injury by affecting the phosphorylation states of proteins in PI3K/Akt/mTOR signaling pathway

  Background: The effect of sevoflurane on the doxorubicin-induced myocardial injury was explored by investigating the phosphorylation states of proteins in phosphatidylinositol 3-kinase (PI3K)/Akt/mam­malian target of rapamycin (mTOR) signaling pathway. Methods: Myocardial injury rat models were induced by doxorubicin and evenly assigned into five groups according to different treatment: Doxorubicin group (DG, 200-μL saline solution), sevoflurane group (SevG, inhaled with 2.4% sevoflurane for 2 h), LY294002 group (LYG, Akt inhibitor, 0.3 mg/kg in 200-μL Dimethyl Sulfoxide [DMSO]), solvent DMSO control group (SG) and autophagy inhibitor 3-methyladenine (3-MA) group (MG, 30 mg/kg in 200-μL DMSO). The healthy rats were assigned to a contro1 group (CG, 200-μL saline solution). Myocardial apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The concentration of cardiac troponin I (cTnI) was detected by ELISA. The levels of total Akt (t-Akt), phosphorylated Akt (p-Akt), mammalian target of rapamycin (mTOR), phosphorylated-mTOR (p-mTOR) and autophagy marker LC3-II was detected by Western Blot. The experiments were also repeated at the cell level. Results: Terminal deoxynucleotidyl transferase dUTP nick end labeling analysis showed that the ap­optosis rates were high in DG and SG, reached the highest level in LYG, reduced in SevG and MG, and reached the lowest level in CG. The levels of p-Akt p-mTOR were low in groups DG and SG, reached the lowest level in LYG, increased in SevG and MG, and reached the highest level in CG. In contrast, LC3-II expression, apoptosis index and serum cTnI concentration were high in DG and SG, reached the highest level in LYG, reduced in SevG and MG, and reached the lowest level in CG (p < 0.05). Cell experiment showed similar results as with animal experiments. Conclusions: Sevoflurane ameliorates myocardial injury by affecting the phosphorylation states of the proteins in PI3K/Akt/mTOR signaling pathway and reducing the injury biomarker. (Cardiol J 2017; 24, 4: 409–418

BIOMECHANICS ANALYSIS OF "BRUSH KNEE AND TWIST STEPS" MOVEMENT IN TAI CHI

The purpose of this study was to analyze the biomechanical characteristics of a typical Tai Chi (TC) movement - "brush knee and twist steps". A 3-Dimensional fixed video filming method was used for data collection. Three elite professional athletes of TC performed this movement three times and the best one was selected for analysis. The kinematics data included the distance of hands and feet, the angle between the feet, the joint angles of wrist, elbow and knee, the 3-dimensional displacement, velocity and acceleration of CG. The analysis showed that TC exercise could enhance the lower extremity muscular strength movement coordination, and the neuromuscular control for posture and balance