Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism

Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe. Like all orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that is encapsidated by the viral nucleocapsid (N) protein in the form of a ribonucleoprotein complex (RNP). We recently reported the three-dimensional structure of SBV N that revealed a novel fold. Here we report the crystal structure of the SBV N protein in complex with a 42-nt-long RNA to 2.16 Å resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ribonucleotides. Eight bases are bound in the positively charged cleft between the N- and C-terminal domains of N, and three bases are shielded by the extended N-terminal arm. SBV N appears to sequester RNA using a different mechanism compared with the nucleoproteins of other negative-sense RNA viruses. Furthermore, the structure suggests that RNA binding results in conformational changes of some residues in the RNA-binding cleft and the N- and C-terminal arms. Our results provide new insights into the novel mechanism of RNA encapsidation by orthobunyaviruses

Z-graded weak modules and regularity

It is proved that if any Z-graded weak module for vertex operator algebra V is completely reducible, then V is rational and C_2-cofinite. That is, V is regular. This gives a natural characterization of regular vertex operator algebras.Comment: 9 page

Quasiperiodic, periodic, and slowing-down states of coupled heteroclinic cycles

We investigate two coupled oscillators, each of which shows an attracting heteroclinic cycle in the absence of coupling. The two heteroclinic cycles are nonidentical. Weak coupling can lead to the elimination of the slowing-down state that asymptotically approaches the heteroclinic cycle for a single cycle, giving rise to either quasiperiodic motion with separate frequencies from the two cycles or periodic motion in which the two cycles are synchronized. The synchronization transition, which occurs via a Hopf bifurcation, is not induced by the commensurability of the two cycle frequencies but rather by the disappearance of the weaker frequency oscillation. For even larger coupling the motion changes via a resonant heteroclinic bifurcation to a slowing-down state corresponding to a single attracting heteroclinic orbit. Coexistence of multiple attractors can be found for some parameter regions. These results are of interest in ecological, sociological, neuronal, and other dynamical systems, which have the structure of coupled heteroclinic cycles

Specification Lasso and an Application in Financial Markets

This paper proposes the method of Specification-LASSO in a flexible semi-parametric regression model that allows for the interactive effects between different covariates. Specification-LASSO extends LASSO and Adaptive Group LASSO to achieve both relevant variable selection and model specification. Specification-LASSO also gives preliminary estimates that facilitate the estimation of the regression model. Monte Carlo simulations show that the Specification-LASSO can accurately specify partially linear additive models with interactive regressors. Finally, the proposed methods are applied in an empirical study, which examines the topic proposed by Freyberger et al. (2020), which argues that firms’ sizes may have interactive effects with other security-specific characteristics, which can explain the stocks excess returns together

Investigation of electrical contact resistance for nonconductive film functionalized with Π -conjugated self-assembled molecules

©2007 American Institute of Physics. The electronic version of this article is the complete one and can be found online at: http://link.aip.org/link/?APPLAB/90/092102/1DOI:10.1063/1.2709638Nonconductive adhesive/nonconductive film (NCA/NCF) bonding technology has attracted increasing research interests as lead-free interconnect. During bonding, heat and pressure are applied and the direct physical contacts between the two surfaces of integrated circuit bump and substrate bond pad can be achieved. The electrical contact resistance of a NCA/NCF joint is controlled by the pressure, roughness and NCA/NCF material properties. An accurate prediction of contact resistance can help guide experiment setup towards improving the electrical performance of NCA/NCF. In this study, a model is developed and correlated to experiments. The effects of NCA/NCF material properties on electrical contact resistance are investigated

Adaptive Measurement Network for CS Image Reconstruction

Conventional compressive sensing (CS) reconstruction is very slow for its characteristic of solving an optimization problem. Convolu- tional neural network can realize fast processing while achieving compa- rable results. While CS image recovery with high quality not only de- pends on good reconstruction algorithms, but also good measurements. In this paper, we propose an adaptive measurement network in which measurement is obtained by learning. The new network consists of a fully-connected layer and ReconNet. The fully-connected layer which has low-dimension output acts as measurement. We train the fully-connected layer and ReconNet simultaneously and obtain adaptive measurement. Because the adaptive measurement fits dataset better, in contrast with random Gaussian measurement matrix, under the same measuremen- t rate, it can extract the information of scene more efficiently and get better reconstruction results. Experiments show that the new network outperforms the original one.Comment: 11pages,8figure