Engineering a portable riboswitch-LacP hybrid device for two-way gene regulation

Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we used a library screening strategy for efficient creation of ON and OFF riboswitches of lacI on the chromosome of Escherichia coli. We then engineered a riboswitch-LacP hybrid device to achieve portable gene control in response to theophylline and IPTG. Moreover, this device regulated target expression in a ‘two-way’ manner: the default state of target expression was ON; the expression was switched off by adding theophylline and restored to the ON state by adding IPTG without changing growth medium. We showcased the portability and two-way regulation of this device by applying it to the small RNA CsrB and the RpoS protein. Finally, the use of the hybrid device uncovered an inhibitory role of RpoS in acetate assimilation, a function which is otherwise neglected using conventional genetic approaches. Overall, this work establishes a portable riboswitch-LacP device that achieves sequential OFF-and-ON gene regulation. The two-way control of gene expression has various potential scientific and biotechnological applications and helps reveal novel gene functions

A Novel Adaptive Spectrum Noise Cancellation Approach for Enhancing Heartbeat Rate Monitoring in a Wearable Device

This paper presents a novel approach, Adaptive Spectrum Noise Cancellation (ASNC), for motion artifacts removal in Photoplethysmography (PPG) signals measured by an optical biosensor to obtain clean PPG waveforms for heartbeat rate calculation. One challenge faced by this optical sensing method is the inevitable noise induced by movement when the user is in motion, especially when the motion frequency is very close to the target heartbeat rate. The proposed ASNC utilizes the onboard accelerometer and gyroscope sensors to detect and remove the artifacts adaptively, thus obtaining accurate heartbeat rate measurement while in motion. The ASNC algorithm makes use of a commonly accepted spectrum analysis approaches in medical digital signal processing, discrete cosine transform, to carry out frequency domain analysis. Results obtained by the proposed ASNC have been compared to the classic algorithms, the adaptive threshold peak detection and adaptive noise cancellation. The mean (standard deviation) absolute error and mean relative error of heartbeat rate calculated by ASNC is 0.33 (0.57) beats·min-1 and 0.65%, by adaptive threshold peak detection algorithm is 2.29 (2.21) beats·min-1 and 8.38%, by adaptive noise cancellation algorithm is 1.70 (1.50) beats·min-1 and 2.02%. While all algorithms performed well with both simulated PPG data and clean PPG data collected from our Verity device in situations free of motion artifacts, ASNC provided better accuracy when motion artifacts increase, especially when motion frequency is very close to the heartbeat rate

Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli

<p>Abstract</p> <p>Background</p> <p>The low pH environment of the human stomach is lethal for most microorganisms; but not <it>Escherichia coli</it>, which can tolerate extreme acid stress. Acid resistance in <it>E. coli </it>is hierarchically controlled by numerous regulators among which are small noncoding RNAs (sncRNA).</p> <p>Results</p> <p>In this study, we individually deleted seventy-nine sncRNA genes from the <it>E. coli </it>K12-MG1655 chromosome, and established a single-sncRNA gene knockout library. By systematically screening the sncRNA mutant library, we show that the sncRNA GcvB is a novel regulator of acid resistance in <it>E. coli</it>. We demonstrate that GcvB enhances the ability of <it>E. coli </it>to survive low pH by upregulating the levels of the alternate sigma factor RpoS.</p> <p>Conclusion</p> <p>GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in <it>E. coli</it>.</p

Observing white dwarf tidal stripping with TianQin gravitational wave observatory

Recently discovered regular X-ray bursts known as quasi-periodic eruptions have a proposed model that suggests a tidal stripping white dwarf inspiralling into the galaxy's central black hole on an eccentric orbit. According to this model, the interaction of the stripping white dwarf with the central black hole would emit gravitational wave signals as well, their detection can help explore the formation mechanism of quasi-periodic eruptions and facilitate multi-messenger observations. In this paper, we aim to perform a preliminary study of the gravitation wave observation of TianQin on this stripping white dwarf model. We investigated the horizon distance of TianQin on this type of gravitation wave signal and found it can be set to 200Mpc. We also find that those stripping white dwarf model sources with central black hole mass within $10^4\sim10^{5.5}M_\odot$ are more likely to be detected by TianQin. We assessed the parameter estimation precision of TianQin on those stripping white dwarf model sources. Our result shows that, even in the worst case, TianQin can determine the central black hole mass, the white dwarf mass, the central black hole spin, and the orbital initial eccentricity with a precision of $10^{-2}$. In the optimistic case, TianQin can determine the central black hole mass and the white dwarf mass with a precision of $10^{-7}$, determine the central black hole spin with a precision of $10^{-5}$, and determine the orbital initial eccentricity with a precision of $10^{-8}$. Moreover, TianQin can determine the luminosity distance with a precision of $10^{-1}$ and determine the sky localization with a precision of $10^{-2}\sim10$ $\rm deg^2$.Comment: 9 pages, 4 figure

All your sessions are belong to us: Investigating authenticator leakage through backup channels on Android

Security of authentication protocols heavily relies on the confidentiality of credentials (or authenticators) like passwords and session IDs. However, unlike browser-based web applications for which highly evolved browsers manage the authenticators, Android apps have to construct their own management. We find that most apps simply locate their authenticators into the persistent storage and entrust underlying Android OS for mediation. Consequently, these authenticators can be leaked through compromised backup channels. In this work, we conduct the first systematic investigation on this previously overlooked attack vector. We find that nearly all backup apps on Google Play inadvertently expose backup data to any app with internet and SD card permissions. With this exposure, the malicious apps can steal other apps' authenticators and obtain complete control over the authenticated sessions. We show that this can be stealthily and efficiently done by building a proof-of-concept app named AuthSniffer. We find that 80 (68.4%) out of the 117 tested top-ranked apps which have implemented authentication schemes are subject to this threat. Our study should raise the awareness of app developers and protocol analysts about this attack vector.No Full Tex

The deformation mechanism in the western Qiangtang terrane and its surroundings: evidence from magnetotelluric data

Located in the central part of the Tibetan Plateau, the Qiangtang terrane preserves important record of the uplift and deformation history of the Plateau, and therefore remains an attractive area of research. However, deep geophysical investigations of its western part are still limited. To further understand the deep structure of the western Qiangtang terrane and its surroundings, we use magnetotelluric array data to generate a 3D electrical structure. It reveals high resistivity anomalies in the upper crust and scattered high conductivity anomalies in the mid-lower crust. The electrical structure also suggests that the Longmu Co-Gozha Co fault once believed to be a major regional deformation boundary, may not have cut through the crust. The melt content and rheological parameters derived from the electrical structures show dominant ductile-type deformation in most of the study area, which contributes to block extrusion along the slip faults. Viscous deformation regions formed by mantle melt upwelling in the mid-lower crust may contribute to the formation of the N-S directed normal faults on the surface

1-[9-Ethyl-6-(2-methyl­benzo­yl)-9H-carbazol-3-yl]ethanone

In the title compound, C24H21NO2, the pendant benzene ring is inclined at a dihedral angle of 86.66 (18)° with respect to the adjacent aromatic ring of the carbozole unit. In the crystal structure, symmetry-related mol­ecules are linked via C—H⋯O and C—H⋯π inter­actions