New simplified analytical method for the Prediction of global stability of steel and composite sway frames

peer reviewedEurocode 4 is the European design code for composite construction; in its so-called EN 1994-1-1 version, the design of “non-sway buildings” is mainly covered. As a result, EC4 focuses on the check of structural elements like beams, columns, slabs and joints. However, in the last years, the construction of taller buildings and larger industrial halls without wind bracing systems tends to make global instability a relevant failure mode, which is not well covered by Eurocode 4. Recently, intensive experimental, numerical and theoretical investigations have been carried out at Liège University. The latter aimed at improving the knowledge in the field of sway composite building frames and at developing appropriate design rules. The rotational behavior of the beam-to-column composite joints is one of the key aspects of the problem to which a special attention has been paid. This paper reflects investigations carried out at Liege University on this topic. In particular, an innovative simplified analytical method to predict the ultimate loading factor and the associated collapse mode of both steel and composite frames subjected to static loadings is presented

Creation and suppression of point defects through a kick-out substitution process of Fe in InP

Indium antisite defect In P-related photoluminescence has been observed in Fe-diffused semi-insulating (SI) InP. Compared to annealed undoped or Fe-predoped SI InP, there are fewer defects in SI InP obtained by long-duration, high-temperature Fe diffusion. The suppression of the formation of point defects in Fe-diffused SI InP can be explained in terms of the complete occupation by Fe at indium vacancy. The In P defect is enhanced by the indium interstitial that is caused by the kick out of In and the substitution at the indium site of Fe in the diffusion process. Through these Fe-diffusion results, the nature of the defects in annealed undoped SI InP is better understood. © 2002 American Institute of Physics.published_or_final_versio

Effects of nutriental and environmental conditions on carotenoid biosynthesis by Rhodotorula sp.

Carotenoid compounds are popular natural antioxidants which are commonly isolated from the plants. Recently, there have been many researches on carotenoid biosynthesis towards low cost products. In this study, Rhodotorula sp. was grown on an agricultural byproduct (corncobs) as a matrix in solid-state fermentation. Essential nutrients were added with different concentrations to optimize condition for the carotenoid biosynthesis. Effects of other environmental factors such as moisture content and fermentation time on the yield were also characterized. The optimal nutrient composition for the yeast’s growth and carotenoid biosynthesis is a compound of 500μg nitrogen and 16mg carbon in 100g matrix. Additionally, the moisture content of 80% is the best for producing carotenoid by this yeast strain. The fermentation time for the highest carotenoid yield is observed after 8 days

Effects of cardiomyopathy-linked mutations K15N and R21H in tropomyosin on thin-filament regulation and pointed-end dynamics

Missense mutations K15N and R21H in striated muscle tropomyosin are linked to dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM), respectively. Tropomyosin, together with the troponin complex, regulates muscle contraction and, along with tropomodulin and leiomodin, controls the uniform thin-filament lengths crucial for normal sarcomere structure and function. We used Förster resonance energy transfer to study effects of the tropomyosin mutations on the structure and kinetics of the cardiac troponin core domain associated with the Ca2+-dependent regulation of cardiac thin filaments. We found that the K15N mutation desensitizes thin filaments to Ca2+ and slows the kinetics of structural changes in troponin induced by Ca2+ dissociation from troponin, while the R21H mutation has almost no effect on these parameters. Expression of the K15N mutant in cardiomyocytes decreases leiomodin’s thin-filament pointed-end assembly but does not affect tropomodulin’s assembly at the pointed end. Our in vitro assays show that the R21H mutation causes a twofold decrease in tropomyosin’s affinity for F-actin and affects leiomodin’s function. We suggest that the K15N mutation causes DCM by altering Ca2+-dependent thin-filament regulation and that one of the possible HCM-causing mechanisms by the R21H mutation is through alteration of leiomodin’s function

Synthesis and photocatalytic property of Prussian blue/g-C3N4 composite applied to degradation of rhodamine B under visible light

In this work, the Prussian blue/g-C3N4 (PB/g-C3N4) composite was synthesized from Prussian blue and g-C3N4 via a simple method. The composite was characterized by using X-ray diffraction, Fourier-transform infrared spectroscopy, and ultraviolet-visible diffuse reflectance spectroscopy. The material’s photocatalytic performance was studied via the degradation of rhodamine B (RhB). The results show that the composite degraded RhB more than pristine Prussian blue under visible light after 60 min. This material is promising for organic waste treatment

Global proteomics analysis of the response to starvation in <i>C. elegans</i>

Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass-spectrometry-based quantitative proteomics to identify global changes in the Caenorhabditis elegans proteome due to acute starvation of young adult animals. Measuring changes in the abundance of over 5,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin-associated proteins, including specific linker histones, histone variants, and histone posttranslational modifications associated with the epigenetic control of gene expression. To maximize community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/)

In Situ Synthesis of Reduced Graphene Oxide and Gold Nanocomposites for Nanoelectronics and Biosensing

In this study, an in situ chemical synthesis approach has been developed to prepare graphene–Au nanocomposites from chemically reduced graphene oxide (rGO) in aqueous media. UV–Vis absorption, atomic force microscopy, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy were used to demonstrate the successful attachment of Au nanoparticles to graphene sheets. Configured as field-effect transistors (FETs), the as-synthesized single-layered rGO-Au nanocomposites exhibit higher hole mobility and conductance when compared to the rGO sheets, promising its applications in nanoelectronics. Furthermore, we demonstrate that the rGO-Au FETs are able to label-freely detect DNA hybridization with high sensitivity, indicating its potentials in nanoelectronic biosensing