2,210 research outputs found
Combining chemical and X-Ray microtomography investigations on crustal xenoliths at Mount Etna: evidence of volcanic gas fluxing
Crustal xenoliths embedded in lavas offer chances to provide information on the lithology of the volcano basement and the process occurring during magma ascent. In this work we studied seven xenoliths found among the scoriae ejected during several paroxysms occurred between 2011 and 2016 at the South East Crater (SEC) of Mount Etna volcano. These samples are characterized by massive presence of glass and vesicles resulting in a foam-like appearance of their texture. A detailed study of their textural and chemical features was carried out combining XRF, SEM-EDS, two-dimensional observation and Synchrotron X-ray microtomography (3D imaging). This multidisciplinary approach allowed us to identify and quantify the components of the samples, individuate the likely provenance rock, ascribable to the Numidian Flysch outcropping west of the Etnean edifice. 2D and 3D imaging were successfully applied to estimate the amount of the phases constituting the samples (grains + glass + vesicles) and the degree of partial melting that occurred during the interaction with the basaltic melt and gaseous phase. The sedimentary rocks underwent profound changes led by partial melting, gas fluxing, vesiculation and selective alkali enrichment. These processes could not be driven exclusively by the prolonged contact with the molten phase but must be considered as result of a significant contribution from the volatiles rising through the plumbing system and the sedimentary basement
Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reactive oxygen species within tissues and cells induces various defense mechanisms or programmed cell death. It has been shown that, besides inducing apoptosis, oxidative stress can also induce autophagy. To date, however, the regulation of autophagy in response to oxidative stress remains largely elusive and poorly understood. Therefore, the present study was designed to examine the ratio between oxidative stress and autophagy in macrophages after oxidant exposure (AAPH) and to investigate the ultrastructural localization of beclin-1, a protein essential for autophagy, under basal and stressful conditions. Our data provide evidence that oxidative stress induces autophagy in macrophages. We demonstrate, for the first time by immunoelectron microscopy, the subcellular localization of beclin-1 in autophagic cells
Effect of argon plasma abutment activation on soft tissue healing: RCT with histological assessment.
OBJECTIVE
To assess the peri-implant soft tissue profiles between argon plasma treatment (PT) and non-treated (NPT) healing abutments by comparing clinical and histological parameters 2 months following abutment placement.
MATERIALS AND METHODS
Thirty participants were randomly assigned to argon-plasma treatment abutments group (PT) or non-treated abutments (NPT) group. Two months after healing abutment placement, soft peri-implant tissues and abutment were harvested, and histological and clinical parameters including plaque index, bleeding on probing, and keratinized mucosa diameter (KM) were assessed. Specialized stainings (hematoxylin-eosin and picrocirious red) coupled with immunohistochemistry (vimentin, collagen, and CK10) were performed to assess soft tissue inflammation and healing, and the collagen content keratinization. In addition to standard statistical methods, machine learning algorithms were applied for advanced soft tissue profiling between the test and control groups.
RESULTS
PT group showed lower plaque accumulation and inflammation grade (6.71% vs. 13.25%, respectively; p-value 0.02), and more advanced connective tissue healing and integration compared to NPT (31.77% vs. 23.3%, respectively; p = 0.009). In the control group, more expressed keratinization was found compared to the PT group, showing significantly higher CK10 (>47.5%). No differences in KM were found between the groups.
SIGNIFICANCE
PT seems to be a promising protocol for guided peri-implant soft tissue morphogenesis reducing plaque accumulation and inflammation, and stimulating collagen and soft tissue but without effects on epithelial tissues and keratinization
Characterization of breast tissues in density and effective atomic number basis via spectral X-ray computed tomography
Differentiation of breast tissues is challenging in X-ray imaging because
tissues might share similar or even the same linear attenuation coefficients
. Spectral computed tomography (CT) allows for more quantitative
characterization in terms of tissue density and effective atomic number by
exploiting the energy dependence of . In this work, 5 mastectomy samples
and a phantom with inserts mimicking breast soft tissues were evaluated in a
retrospective study. The samples were imaged at three monochromatic energy
levels in the range of 24 - 38 keV at 5 mGy per scan using a propagation-based
phase-contrast setup at SYRMEP beamline at the Italian national synchrotron
Elettra. A custom-made algorithm incorporating CT reconstructions of an
arbitrary number of spectral energy channels was developed to extract the
density and effective atomic number of adipose, fibro-glandular, pure
glandular, tumor, and skin from regions selected by a radiologist. Preliminary
results suggest that, via spectral CT, it is possible to enhance tissue
differentiation. It was found that adipose, fibro-glandular and tumorous
tissues have average effective atomic numbers (5.94 0.09, 7.03
0.012, and 7.40 0.10) and densities (0.90 0.02, 0.96 0.02,
and 1.07 0.03 g/cm) and can be better distinguished if both
quantitative values are observed together.Comment: 26 pages, 7 figures, submitted to Physics in Medicine and Biolog
Meniscal ossicles as micro-CT imaging biomarker in a rodent model of antigen-induced arthritis: A synchrotron-based x-ray pilot study
It is increasingly recognized that early detection of bone erosion plays an important role in the overall evaluation of rheumatoid arthritis and in the choice of the correct treatment approach. Since an appropriate use of imaging biomarkers in preclinical settings offers the prospect of smaller and optimized sample size, in the present study we define an anatomical imaging biomarker that could be objectively measured from micro-CT imaging data as an indicator of bone erosion in arthritis process. The well-characterized antigen-induced arthritis (AIA) model in rats was used. The animals were divided into 2 groups: arthritic disease control and arthritic having been administrated with the tumor necrosis factor alpha-blocking agent (Humira). Rats were sacrificed in the acute phase of AIA; peripheral blood and synovial tissue were collected for assessment of arthritis. Ex vivo micro-CT tomography of knee joints was performed at the Elettra synchrotron light source (Trieste, Italy). Overall, results from this study suggest that use of high-resolution micro-CT analysis coupled with meniscal ossicles bone parameters quantification provide a powerful combination to enhance data interpretation and assessment of disease-modifying drugs in an animal model of arthritis
Influence of Chemical Composition and Microvesiculation on the Chromatic Features of the Obsidian of Sierra de las Navajas (Hidalgo, Mexico)
The obsidian of Sierra de las Navajas is well known for its green color and gold hue. In order to relate these features with compositional and microtextural characteristics, we have carried out a microanalytical study by Wave Dispersion System associated to Electron Probe Micro-Analyzer, Scanning Electron Microscope observation, and X-rays micro-tomographic analyses of samples showing different colors (dark to light green, sometimes with bands of different color intensity) and hues (changing, uniform, no hue). In accordance with previous studies, the green color of the obsidian seems to be related to a high iron content, probably in its reduced state. However, no significant difference in composition occurs between dark and light green samples. The SEM observation and microtomographic study revealed the absence of microcrystals and the occurrence of vesicles of different size, shape, and orientation. Lighter green colors are shown by highly vesiculated surfaces, whereas non-vesiculated samples are darker. On the surfaces with a high concentration of coarse vesicles, a uniform golden hue is observed. Decreasing vesiculation gives a hue changing with the angle of incident light. However, when the vesicularity approaches zero, no hue is visible. The iso-orientation of vesicles along preferential directions and their distribution in bands determine the variation in color intensity and hue on differently oriented surfaces. Microvesiculation also influences other characteristics that were important features for the use of obsidian in the past, such as fracture, transparency, and roughness
Distribution of corpora amylacea in the human midbrain: using synchrotron radiation phase-contrast microtomography, high-field magnetic resonance imaging, and histology
Corpora amylacea (CA) are polyglucosan aggregated granules that accumulate in the human body throughout aging. In the cerebrum, CA have been found in proximity to ventricular walls, pial surfaces, and blood vessels. However, studies showing their three-dimensional spatial distribution are sparse. In this study, volumetric images of four human brain stems were obtained with MRI and phase-contrast X-ray microtomography, followed up by Periodic acid Schiff stain for validation. CA appeared as hyperintense spheroid structures with diameters up to 30μm. An automatic pipeline was developed to segment the CA, and the spatial distribution of over 200,000 individual corpora amylacea could be investigated. A threefold—or higher—density of CA was detected in the dorsomedial column of the periaqueductal gray (860–4,200 CA count/mm3) than in the superior colliculus (150–340 CA count/mm3). We estimated that about 2% of the CA were located in the immediate vicinity of the vessels or in the peri-vascular space. While CA in the ependymal lining of the cerebral aqueduct was rare, the sub-pial tissue of the anterior and posterior midbrain contained several CA. In the sample with the highest CA density, quantitative maps obtained with MRI revealed high R2∗ values and a diamagnetic shift in a region which spatially coincided with the CA dense region
X-ray and FTIR \u3bc-CTs for morphological and chemical characterization of eco-sustainable insulating foams
Here it is reported a multidisciplinary approach based on tomography and infrared techniques applied to the characterization of tannin porous rigid foams, potentially usable as new insulating materials in green building technology. With conventional x-ray tomography it was possible to preliminary evaluate the homogeneity of the samples at low resolution, while then, thanks to the synchrotron source, it was possible to obtain more detailed information at a micro-scale level. At the same time chemical characterization was done through Fourier Transform infrared (FTIR) imaging. Conventionally, FTIR imaging is limited to a planar projection, not considering the 3D structure of the material. To avoid this limitation, a FTIR 3D-tomography setup was built and the foams characterized by a chemical point of view. The idea is to directly correlate these data with the 3D-structural information obtained with the x-ray computed tomography exploiting the synchrotron radiation as source, allowing a complete characterization of the material morphology and chemistry at the microscale
Synthesis and Characterization of High-Performing Sulfur-Free Tannin Foams
Tannin foams are green lightweight materials that have attracted industrial interest for the
manufacturing of sandwich panels for insulation purposes. However, the dimensions of the cells and
the presence of sulfur in the formulation developed until now have discouraged their upscaling. In
this work, we present the synthesis and the characterization of the more promising small cell and
sulfur-free materials. It was observed that, with respect to standard ones, foams catalyzed with nitric
acid present similar physical properties and more phenolic character, which favors the absorption of
ionic pollutants. Conversely, the foams blown with aliphatic solvents and surfactants present smaller
pores, and higher mechanical and insulating properties, without a\ufb00ecting the chemical properties or
the heating value. The combined foam produced with nitric acid as a catalyst and petroleum ether as
a blowing agent result in sulfur-free and small cell material with overall improved features. These
foams have been produced at 30
7 30
7 3 cm3, with high homogeneity and, to date, they represent
the most suitable formulation for industrial upscaling
Raspberry Pi Driven Flow-Injection System for Electrochemical Continuous Monitoring Platforms
Nowadays, there is an immense interest regarding new bio-sensing technologies, highlighting the need for effective validation of their capabilities. This necessity is more crucial when examining the proprieties of a sensor for continuous monitoring of a concentration trend in time, before in vivo implementations. In the framework of personalised medicine, it is imperative to introduce a robust way to parametrise the highly variable responses of human metabolism. We propose a novel solution for the design of an automatic flow-injection environment that validates continuous monitoring systems performances. The setup is also validated for reproducing a paracetamol concentration trend in buffer solution
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