Genome-Wide Meta-Analysis Identifies Variants in DSCAM and PDLIM3 that correlate with efficacy outcomes in metastatic renal cell carcinoma patients treated with sunitinib

Individual response to sunitinib in metastatic renal cell carcinoma (mRCC) patients is highly variable. Earlier, sunitinib outcome was related to single nucleotide polymorphisms (SNPs) in CYP3A5 and ABCB1. Our aim is to provide novel insights into biological mechanisms underlying sunitinib action. We included mRCC patients from the European EuroTARGET consortium (n = 550) and the RIKEN cohort in Japan (n = 204) which were analysed separately and in a meta-analysis of genome-wide association studies (GWAS). SNPs were tested for association with progression-free survival (PFS) and overall survival (OS) using Cox regression. Summary statistics were combined using a fixed effect meta-analysis. SNP rs28520013 in PDLIM3 and the correlated SNPs rs2205096 and rs111356738 both in DSCAM, showed genome-wide significance (p Personalised Therapeutic

A revised method for the preparation of dry bone samples used in histological examination: Five simple steps

Histology of dry bone tissue has many scientific applications. The histological analysis of bone requires the production of good quality thin sections. Many researchers have developed new histological techniques and/or they have refined existing ones. In this paper, we describe a revision of histological techniques for obtaining thin sections from modern dry bone. The method is easy to apply and the equipment required is commonly found in a histology laboratory. In comparison to other techniques presented in the literature, this adapted method reduces the number of consumables and steps, thereby improving the efficiency and cost-effectiveness of the procedure