Le FORUM, Vol. 37 No. 4

https://digitalcommons.library.umaine.edu/francoamericain_forum/1039/thumbnail.jp

Novel roles for podocalyxin in regulating stress myelopoiesis, Rap1a, and neutrophil migration.

Podocalyxin (Podxl) is a CD34 orthologue and cell surface sialomucin reported to have roles in renal podocyte diaphragm slit development; vascular cell integrity; and the progression of blood, breast, and prostate cancers. Roles for Podxl during nonmalignant hematopoiesis, however, are largely undefined. We have developed a Vav-Cre Podxl knockout (KO) mouse model, and report on novel roles for Podxl in governing stress myelopoiesis. At steady state, Podxl expression among hematopoietic progenitor cells was low level but was induced by granulocyte colony-stimulating factor (G-CSF) in myeloid progenitors and by thrombopoietin in human stem cells. In keeping with low-level Podxl expression at steady state, Vav-Cre deletion of Podxl did not markedly alter peripheral blood cell levels. A G-CSF challenge in Podxl-KO mice, in contrast, hyperelevated peripheral blood neutrophil and monocyte levels. Podxl-KO also substantially heightened neutrophil levels after 5-fluorouracil myeloablation. These loss-of-function phenotypes were selective, and Podxl-KO did not alter lymphocyte, basophil, or eosinophil levels. Within bone marrow (and after G-CSF challenge), Podxl deletion moderately decreased colony forming units-granulocytes, eyrthrocytes, monocyte/macrophages, megakaryocytes and CD16/3

Video1_Observations of ion upflow and 630.0 nm emission during pulsating aurora.MPG

In this study, we report observations made by filtered (557.7 and 630.0 nm) All-Sky Imagers located at Poker Flat, Alaska alongside Poker Flat Incoherent Scatter Radar data for an event observed on 5 February 2017. Together, the data indicate ion upflow in the vicinity of pulsating aurora. Additionally, the data show a strong 630.0 nm (red-line) auroral emission. Observations of pulsating aurora are typically reported at 557.7 and 427.8 nm, as these wavelengths are more sensitive to high-energy (∼ tens of keV) electron precipitation. In contrast, 630.0 nm emission is generated preferentially by low-energy soft electron precipitation (∼ hundreds of eV), and is less commonly observed. The All-Sky Imager data discussed here are unusual in that they suggest regions of enhanced soft electron precipitation in conjunction with enhanced ambipolar electric fields, which are a known factor contributing to ion outflow.</p