1,110 research outputs found
Perturbations in the E-cadherin/catenin junctional complex in mouse embryos resulting in exencephaly
Finite element simulations of the deformation of fused-cast refractories based on X-ray computed tomography
International audienceThis work consists in building a 3D numerical model of the microstructure of a fused-cast refractory (two-phase material)..
Enhanced Intrinsic Excitability in Basket Cells Maintains Excitatory-Inhibitory Balance in Hippocampal Circuits
SummaryThe dynamics of inhibitory circuits in the cortex is thought to rely mainly on synaptic modifications. We challenge this view by showing that hippocampal parvalbumin-positive basket cells (PV-BCs) of the CA1 region express long-term (>30 min) potentiation of intrinsic neuronal excitability (LTP-IEPV-BC) upon brief repetitive stimulation of the Schaffer collaterals. LTP-IEPV-BC is induced by synaptic activation of metabotropic glutamate receptor subtype 5 (mGluR5) and mediated by the downregulation of Kv1 channel activity. LTP-IEPV-BC promotes spiking activity at the gamma frequency (∼35 Hz) and facilitates recruitment of PV-BCs to balance synaptic and intrinsic excitation in pyramidal neurons. In conclusion, activity-dependent modulation of intrinsic neuronal excitability in PV-BCs maintains excitatory-inhibitory balance and thus plays a major role in the dynamics of hippocampal circuits
The OPFOS microscopy family: High-resolution optical-sectioning of biomedical specimens
We report on the recently emerging (Laser) Light Sheet based Fluorescence
Microscopy field (LSFM). The techniques used in this field allow to study and
visualize biomedical objects non-destructively in high-resolution through
virtual optical sectioning with sheets of laser light. Fluorescence originating
in the cross section of the sheet and sample is recorded orthogonally with a
camera. In this paper, the first implementation of LSFM to image biomedical
tissue in three dimensions - Orthogonal-Plane Fluorescence Optical Sectioning
microscopy (OPFOS) - is discussed. Since then many similar and derived methods
have surfaced (SPIM, Ultramicroscopy, HR-OPFOS, mSPIM, DSLM, TSLIM...) which we
all briefly discuss. All these optical sectioning methods create images showing
histological detail. We illustrate the applicability of LSFM on several
specimen types with application in biomedical and life sciences.Comment: 19 pages, 10 figures, to be published in Anatomical Research
International (Hindawi
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