Investigation of sperm chromatin condensation by aniline blue staining in infertile men with normal and abnormal semen parameters

Aim: To evaluate the relationship between sperm chromatin condensation assessed by aniline blue staining and semen parameters in infertile men. Methods: Infertile men applied to our urology clinics and diagnosed with normozoospermia (n=50), asthenozoospermia (n=17), oligozoospermia (n=3), teratozoospermia (n=2) oligoasthenoteratozoospermia (OAT) (n=10) according to their semen analysis were included in the study. Semen samples were evaluated for sperm chromatin condensation by aniline blue staining. Results: The percentage of aniline-positive spermatozoa in the OAT and teratozoospermia group was found to be higher than in the normozoospermic and asthenozoospermic infertile groups (p=0.002, p=0.044 with normozoospermia group and p=0.026, p=0.007 with asthenozoospermia group, respectively). Sperm chromatin condensation was negatively correlated with sperm concentration (p=0.003, r=-0.322), total sperm count (p=0.004, r=-0.313), total progressive motile sperm count (p=0.005, r=-0.307), and normal morphology (p<0.0001, r=-0.554); and positively correlated with the percentage of immotile sperm (p=0.037, r=0.230). Conclusion: Sperm chromatin condensation was found to be different in infertile men differently diagnosed based on their semen analysis. The results of the study suggest that chromatin condensation, together with routine sperm parameters, may constitute a valuable parameter in the evaluation of male fertility

Social and psychological well-being among vulnerable groups during covid-19 epidemic: Literature review

While traumatic Covid-19 epidemic affects many people’s everyday lives, it is very important to recognize vulnerable groups’ needs during coronavirus cri-sis. Individuals’ social-emotional, psychological, spiritual-religious and economic needs in coronavirus crisis should be examined. Vulnerable groups often face ad-ditional struggles during traumatic events. This article aims to investigate avail-able evidence on key experiences of social and psychological well-being among vulnerable groups including elderly people, children, youths and pregnant women during the pandemic. The critical literature review was the method of this article. This investigation concentrates on vulnerable groups’ key lived experiences dur-ing lockdown linked to the outbreak. Also, it examines available literature on how elderly people, children, youths and pregnant women face Covid-19 pandemic restrictions and lockdown by focusing on their wellness. This article found that vulnerability among children, young people, pregnant women and elderly people was associated with their developmental circumstances. Importantly, these groups were frequently face social and psychological difficulties based on their vulnerable positions during coronavirus crisis. It suggests that social policymakers need to develop available and accessible tools and sources for vulnerable groups during Covid-19 to meet their social and psychological needs.

Covid-19 in Liver Transplant Recipients: A National Cohort

[No Abstract Available