Antimicrobial Activity of Sabulun Salo a Local Traditional Medicated Soap

The antimicrobial activity of Sabulun salo; a local traditional medicated soap widely used by different tribes in Nigeria such as Hausa, Yoruba and Nupe against skin infections was examined against some clinical isolates of pathogenic microorganisms (Staphylococcus aureus, Escherichia coli and Candida albicans) using agar dilution method. The pattern of inhibition varied with the soap concentration and the organisms tested. The soap was more effective on S. aureus with maximum zone of growth inhibition of 28 mm at 100% w/v followed by C. albicans (24mm). However, E. coli was resistant to the soap at all concentrations tested. The minimum inhibitory concentration (MIC) was found to be 12.5% w/v for both S. aureus and C. albicans. The antibacterial activities exhibited by sabulun salo in this study could be attributed to the presence of its constituents which signifies the potential of the soap as a of topical therapeutic agent. These findings therefore, justify the traditional medicinal use of sabulun salo

Phytochemical and Antibacterial Properties of Leaf Extracts of Ipomoea asarifolia

The antibacterial potency of aqueous and methanol extracts of Ipomoea asarifolia leaves; a plant widely used by traditional medical practitioners in Nigeria was determined in vitro against three bacterial pathogens (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) by agar well diffusion method. The pattern of inhibition varied with the extracts and the organisms tested. Both the aqueous and methanol extracts were potent on E. coli and S. aureus with maximum zone of growth inhibition of 21mm and 20mm at 200mg/ml respectively Pseudomonas aeruginosa was resistant to both extracts at all the concentrations tested. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) against E. coli for aqueous extract were 50mg/ml and 100mg/ml respectively; and 100mg/ml and 200mg/ml for methanol extract respectively. Similarly, The MIC and MBC against S. aureus for aqueous extract were 100mg/ml and 200mg/ml; 200mg/ml for the methanol extract. Preliminary phytochemical screening reveal the presence of anthraquinones, saponins and tannins in both aqueous and methanol extracts; triterpenes, flavonoids and glycosides were found only in the aqueous extract while alkaloids were found only in the methanol extract. However, carbohydrate and steroids were absent in both extracts. The spectra of activities shown by the extracts could be attributed to the presence of these phytochemicals which signifies the potential of I. asarifolia as a source of therapeutic agents. These findings therefore, justify the traditional medicinal use of the plant.Keywords: Ipomoea asarifolia, phytochemical, antibacterial potency, bacterial pathogensNigerian Journal of Basic and Applied Science (2011), 19 (2): 236-24

Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection

Abstract Fumonisins B1 (FB1) and fumonisin B2 (FB2) are the main members of a family of mycotoxins produced by Fusarium verticillioides, Fusarium proliferatum, and other fungi species of the section Liseola. The present work shows the results of comparative studies using two different procedures for the analysis of fumonisins in maize and maize-based samples. The studied analytical methods involve extraction with methanol/water, dilution with PBS, and clean-up through immunoaffinity columns. Two reagents (o-phthaldialdehyde and naphthalene-2,3-dicarboxaldehyde) were studied for formation of fluorescent derivatives. The separation and identification were carried out by high-performance liquid chromatography with fluorescence detection. The optimized method for analysis of fumonisins in maize involved extraction with methanol/water (80:20), clean-up with an immunoaffinity column, and derivatization with naphthalene-2,3-dicarboxaldehyde (NDA). The limit of detection was 20 µg kg-1 for FB1 and 15 µg kg-1 for FB2. Recoveries of FB1 and FB2 ranged from 79% to 99.6% for maize fortified at 150 µg kg-1 and 200 µg kg-1, respectively, with within-day RSDs of 3.0 and 2.7%. The proposed method was applied to 31 samples, and the presence of fumonisins was found in 14 samples at concentrations ranging from 113 to 2,026 µg kg-1. The estimated daily intake of fumonisins was 0.14 µg kg-1 body weight per day

Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions

The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.The authors thank the Brazilian Agricultural Research Corporation (EMBRAPA/Brazil), National Council for Scientific and Technological Development (CNPq/Brazil), Agency for the Improvement of Higher Education Studies (CAPES/Brazil) and Sergipe State Research and Technological Innovation Foundation (FAPITEC/SE, Brazil) for their financial support. The authors also acknowledge the valuable contributions of Dr. Sarah Brandao Santa Cruz Barbosa (in memoriam) during the execution of this study