Claiming spaces: Prioritising Maaori worldview

Ohomairangi Trust was established as a provider of early intervention services in February 2002. It is funded and accredited by the Ministry of Education, and is the first Kaupapa Maaori based early intervention service to be accredited by the Ministry of Education. Essentially Ohomairangi was developed because of a need in the community for a service that could focus on developing and providing early intervention in a uniquely Maaori way, without the constraints of a crown agency. The Ohomairangi early intervention team has a commitment to supporting both the positive developments for Maaori within the Ministry of Education, and the continued independent research and development of Kaupapa Maaori services. The primary purpose of Ohomairangi is to develop and provide a Kaupapa Maaori based early intervention service across Taamaki Makaurau, which meets recommended practice guidelines. This evolves from a starting point of Kaupapa Maaori theory

Sustainable DC

Over the past three decades, sustainability has earned a growing importance in city planning and policy decisions. Planners often champion sustainable development as the model framework for achieving social, economic and environmental objectives. However, sustainability in practice is less about striking the perfect balance between these three components than it is about building livable cities. In 2011, Washington DC launched the Sustainable DC initiative. This vision sought to identify focus areas and goals for making the District "the healthiest, greenest and most livable city in the United States" by 2030. The purpose of this thesis is to understand ways in which the city can ensure a high quality of life for residents as it works to implement the Sustainable DC initiative. This research examined relationships between the physical environment and socioeconomic characteristics of residents across DC neighborhoods to make recommendations for implementing Sustainable DC

Assessing an Age-Graded Theory of Informal Social Control: Are There Conditional Effects of Life Events in the Desistance Process?

In 1993, Sampson and Laub presented their age-graded theory of informal social control in Crime in the Making: Pathways and Turning Points Through Life. In essence, Sampson and Laub state that, among offenders, strong social bonds stemming from a variety of life events predict desistance from criminal offending in adulthood. In the past decade, there has been a growing amount of research supporting this general finding. However, little research has examined the potential conditional effects of life events on desistance. Using Sheldon and Eleanor Gluecks' Unraveling Juvenile Delinquency data, their follow-up data to age 32, and the long-term follow-up data collected by John Laub and Robert Sampson, this research focuses on the potential conditional effects of marital attachment, stable employment, honorable military service, and long-term juvenile incarceration on criminal offending over the life course. Specifically, the present study tests Sampson and Laub's notion that strong social bonds predict desistance by asking two fundamental questions that bear on both theory and policy surrounding desistance from crime. First, does a high level of social integration as evidenced by the accumulation of social bonds stemming from life events within the same individual influence a person's level of offending and/or rate of desistance? Second, does the individual risk factor of low self-control or the related protective factor of adolescent competence interact with life events such that they differentially influence adult offending patterns? Using the longitudinal methodologies of semiparametric mixed Poisson modeling and hierarchical linear modeling, the analyses find additional support for Sampson and Laub's theory. First, a person's level of social integration significantly affects his future offending patterns even after controlling for criminal propensity and prior adult crime. Second, no significant interaction effects emerge between life events and individual characteristics on future offending patterns. The conclusion then is that a high level of social bonding within the same individual influences offending, regardless of a person's level of self-control or adolescent competence. The implications of this research for life-course theories of crime, future research, and policies regarding desistance are discussed

Diagnostic Screening Workflow for Mutations in the BRCA1 and BRCA2 Genes

Objectives: Screening for mutations in large genes is challenging in a molecular diagnostic environment. Sanger-based DNA sequencing methods are largely used; however, massively parallel sequencing (MPS) can accommodate increasing test demands and financial constraints. This study aimed to establish a simple workflow to amplify and screen all coding regions of the BRCA1 and BRCA2 (BRCA1/2) genes by Sanger-based sequencing as well as to assess a MPS approach encompassing multiplex polymerase chain reaction (PCR) and pyrosequencing. Methods: This study was conducted between July 2011 and April 2013. A total of 20 patients were included in the study who had been referred to Genetic Health Services New Zealand (Northern Hub) for BRCA1/2 mutation screening. Patients were randomly divided into a MPS evaluation and validation cohort (n = 10 patients each). Primers were designed to amplify all coding exons of BRCA1/2 (28 and 42 primer pairs, respectively). Primers overlying known variants were avoided to circumvent allelic drop-out. The MPS approach necessitated utilisation of a complementary fragment analysis assay to eliminate apparent false-positives at homopolymeric regions. Variants were filtered on the basis of their frequency and sequence depth. Results: Sanger-based sequencing of PCRamplified coding regions was successfully achieved. Sensitivity and specificity of the combined MPS/homopolymer protocol was determined to be 100% and 99.5%, respectively. Conclusion: In comparison to traditional Sangerbased sequencing, the MPS workflow led to a reduction in both cost and analysis time for BRCA1/2 screening. MPS analysis achieved high analytical sensitivity and specificity, but required complementary fragment analysis combined with Sanger-based sequencing confirmation in some instances

PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA replication

DNA damage can stall the DNA replication machinery, leading to genomic instability. Thus, numerous mechanisms exist to complete genome duplication in the absence of a pristine DNA template, but identification of the enzymes involved remains incomplete. Here, we establish that Primase-Polymerase (PrimPol; CCDC111), an archaeal-eukaryotic primase (AEP) in eukaryotic cells, is involved in chromosomal DNA replication. PrimPol is required for replication fork progression on ultraviolet (UV) lightdamaged DNA templates, possibly mediated by its ability to catalyze translesion synthesis (TLS) of these lesions. This PrimPol UV lesion bypass pathway is not epistatic with the Pol h-dependent pathway and, as a consequence, protects xeroderma pigmentosum variant (XP-V) patient cells from UV-induced cytotoxicity. In addition, we establish that PrimPol is also required for efficient replication fork progression during an unperturbed S phase. These and other findings indicate that PrimPol is an important player in replication fork progression in eukaryotic cells

Prevalence, knowledge and factors associated with e-cigarette use among parents of secondary school children

OBJECTIVES Identify prevalence rates and attitudes towards e-cigarette use among parents to inform prevention strategies designed to reduce uptake in young people. STUDY DESIGN A mixed methods sequential study guided by the Theory of Planned Behaviour. METHODS This research involved two phases. Phase one was an elicitation study using focus groups, interviews and open-ended questionnaires (N = 17) to elicit parental behavioural, normative, and control beliefs around e-cigarette use. Findings from phase 1 were used to inform a questionnaire administered to a sample of 612 parents in phase 2. The aim of phase 2 was to identify and explain factors that influence parental attitudes and motivations towards e-cigarette use. Parents were recruited through post-primary schools and were sent a link to an online survey. RESULTS Approximately 19% of parents had tried an e-cigarette, with 9% reporting current use. Sociodemographic variables, TPB constructs and knowledge of e-cigarettes, accounted for 43% and 60% of ever use and intention to use an e-cigarette, respectively. Intention, gender, age and free school meal entitlement were associated with ever use. Intention to use an e-cigarette was related to lower educational level, current smoking of traditional cigarettes, more positive attitudes, greater social pressure, having greater control over use and knowledge. CONCLUSIONS Prevention strategies designed to reduce uptake in young people should raise awareness of the health risks of e-cigarette use, legislation and regulations and highlight the role parents play in encouraging young people to abstain from using an e-cigarette

Array-based Identification of Copy Number Changes in a Diagnostic Setting : Simultaneous gene-focused and low resolution whole human genome analysis

Objectives: The aim of this study was to develop and validate a comparative genomic hybridisation (CGH) array that would allow simultaneous targeted analysis of a panel of disease genes and low resolution whole genome analysis. Methods: A bespoke Roche NimbleGen 12x135K CGH array (Roche NimbleGen Inc., Madison, Wisconsin, USA) was designed to interrogate the coding regions of 66 genes of interest, with additional widelyspaced backbone probes providing coverage across the whole genome. We analysed genomic deoxyribonucleic acid (DNA) from 20 patients with a range of previously characterised copy number changes and from 8 patients who had not previously undergone any form of dosage analysis. Results: The custom-designed Roche NimbleGen CGH array was able to detect known copy number changes in all 20 patients. A molecular diagnosis was also made for one of the additional 4 patients with a clinical diagnosis that had not been confirmed by sequence analysis, and carrier testing for familial copy number variants was successfully completed for the remaining four patients. Conclusion: The custom-designed CGH array described here is ideally suited for use in a small diagnostic laboratory. The method is robust, accurate, and cost-effective, and offers an ideal alternative to more conventional targeted assays such as multiplex ligation-dependent probe amplification