The pleiotropic deubiquitinase Ubp3 confers aneuploidy tolerance

Aneuploidy-or an unbalanced karyotype in which whole chromosomes are gained or lost-causes reduced fitness at both the cellular and organismal levels but is also a hallmark of human cancers. Aneuploidy causes a variety of cellular stresses, including genomic instability, proteotoxic and oxidative stresses, and impaired protein trafficking. The deubiquitinase Ubp3, which was identified by a genome-wide screen for gene deletions that impair the fitness of aneuploid yeast, is a key regulator of aneuploid cell homeostasis. We show that deletion of UBP3 exacerbates both karyotype-specific phenotypes and global stresses of aneuploid cells, including oxidative and proteotoxic stress. Indeed, Ubp3 is essential for proper proteasome function in euploid cells, and deletion of this deubiquitinase leads to further proteasome-mediated proteotoxicity in aneuploid yeast. Notably, the importance of UBP3 in aneuploid cells is conserved. Depletion of the human homolog of UBP3, USP10, is detrimental to the fitness of human cells upon chromosome missegregation, and this fitness defect is accompanied by autophagy inhibition. We thus used a genome-wide screen in yeast to identify a guardian of aneuploid cell fitness conserved across species. We propose that interfering with Ubp3/USP10 function could be a productive avenue in the development of novel cancer therapeutics

Phase Transitions in Isolated Vortex Chains

In very anisotropic layered superconductors (e.g. Bi$_2$Sr$_2$CaCu$_2$O$_x$) a tilted magnetic field can penetrate as two co-existing lattices of vortices parallel and perpendicular to the layers. At low out-of-plane fields the perpendicular vortices form a set of isolated vortex chains, which have recently been observed in detail with scanning Hall-probe measurements. We present calculations that show a very delicate stability of this isolated-chain state. As the vortex density increases along the chain there is a first-order transition to a buckled chain, and then the chain will expel vortices in a continuous transition to a composite-chain state. At low densities there is an instability towards clustering, due to a long-range attraction between the vortices on the chain, and at very low densities it becomes energetically favorable to form a tilted chain, which may explain the sudden disappearance of vortices along the chains seen in recent experiments.Comment: 9 pages, 10 figure

The Value of Inherited Deficiencies of Human Carbonic Anhydrase Isozymes in Understanding Their Cellular Roles a

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73265/1/j.1749-6632.1984.tb12346.x.pd

Evaporation of the pancake-vortex lattice in weakly-coupled layered superconductors

We calculate the melting line of the pancake-vortex system in a layered superconductor, interpolating between two-dimensional (2D) melting at high fields and the zero-field limit of single-stack evaporation. Long-range interactions between pancake vortices in different layers permit a mean-field approach, the ``substrate model'', where each 2D crystal fluctuates in a substrate potential due to the vortices in other layers. We find the thermal stability limit of the 3D solid, and compare the free energy to a 2D liquid to determine the first-order melting transition and its jump in entropy.Comment: 4 pages, RevTeX, two postscript figures incorporated using eps

Vortex Collisions: Crossing or Recombination?

We investigate the collision of two vortex lines moving with viscous dynamics and driven towards each other by an applied current. Using London theory in the approach phase we observe a non-trivial vortex conformation producing anti-parallel segments; their attractive interaction triggers a violent collision. The collision region is analyzed using the time-dependent Ginzburg-Landau equation. While we find vortices will always recombine through exchange of segments, a crossing channel appears naturally through a double collision process.Comment: 4 pages, 3 figure

Vortices in a Thin Film Superconductor with a Spherical Geometry

We report results from Monte Carlo simulations of a thin film superconductor in a spherical geometry within the lowest Landau level approximation. We observe the absence of a phase transition to a low temperature vortex solid phase with these boundary conditions; the system remains in the vortex liquid phase for all accessible temperatures. The correlation lengths are measured for phase coherence and density modulation. Both lengths display identical temperature dependences, with an asymptotic scaling form consistent with a continuous zero temperature transition. This contrasts with the first order freezing transition which is seen in the alternative quasi-periodic boundary conditions. The high temperature perturbation theory and the ground states of the spherical system suggest that the thermodynamic limit of the spherical geometry is the same as that on the flat plane. We discuss the advantages and drawbacks of simulations with different geometries, and compare with current experimental conclusions. The effect of having a large scale inhomogeneity in the applied field is also considered.Comment: This replacment contains substantial revisions: the new article is twice as long with new and different results on the thermodynamic limit on the sphere plus a full discussion on the alternative boundary conditions used in simulations in the LLL approximation. 19 pages, 12 encapsulated PostScript figures, 1 JPEG figure, uses RevTeX (with epsf

Josephson vortices and solitons inside pancake vortex lattice in layered superconductors

In very anisotropic layered superconductors a tilted magnetic field generates crossing vortex lattices of pancake and Josephson vortices (JVs). We study the properties of an isolated JV in the lattice of pancake vortices. JV induces deformations in the pancake vortex crystal, which, in turn, substantially modify the JV structure. The phase field of the JV is composed of two types of phase deformations: the regular phase and vortex phase. The phase deformations with smaller stiffness dominate. The contribution from the vortex phase smoothly takes over with increasing magnetic field. We find that the structure of the cores experiences a smooth yet qualitative evolution with decrease of the anisotropy. At large anisotropies pancakes have only small deformations with respect to position of the ideal crystal while at smaller anisotropies the pancake stacks in the central row smoothly transfer between the neighboring lattice positions forming a solitonlike structure. We also find that even at high anisotropies pancake vortices strongly pin JVs and strongly increase their viscous friction.Comment: 22 pages, 11 figures, to appear in Phys. Rev.

Topological Defects in the Abrikosov Lattice of Vortices in Type-II Superconductors

The free energy costs for various defects within an Abrikosov lattice of vortices are calculated using the lowest Landau level approximation (LLL). Defect solutions with boundary conditions for lines to meet at a point (crossing defect) and for lines to twist around each other (braid defect) are sought for 2, 3, 6, and 12 lines. Many results have been unexpected, including the nonexistence of a stable two- or three-line braid. This, and the high energy cost found for a six-line braid lead us to propose that the equilibrium vortex state is not entangled below the irreversibility line of the high-$T_c$ superconductors or in a large part of the vortex-liquid phase above this line. Also, the solution for an infinite straight screw dislocation is found, and used to give a limiting form for the free energy cost of very large braids. This depends on the area enclosed by the braid as well as its perimeter length.Comment: 30 pages, 17 Encapsulated PostScript figures, uses Revtex (with epsf

Abrupt Change of Josephson Plasma Frequency at the Phase Boundary of the Bragg Glass in Bi_2Sr_2CaCu_2O_{8+\delta}

We report the first detailed and quantitative study of the Josephson coupling energy in the vortex liquid, Bragg glass and vortex glass phases of Bi_2Sr_2CaCu_2O_{8+\delta} by the Josephson plasma resonance. The measurements revealed distinct features in the T- and H-dependencies of the plasma frequency $\omega_{pl}$ for each of these three vortex phases. When going across either the Bragg-to-vortex glass or the Bragg-to-liquid transition line, $\omega_{pl}$ shows a dramatic change. We provide a quantitative discussion on the properties of these phase transitions, including the first order nature of the Bragg-to-vortex glass transition.Comment: 5pages, 4figure