Antiretroviral therapy among HIV-infected persons in Northeastern Vietnam : Impact of peer support on virologic failure and mortality in a cluster randomized controlled trial

Background: Wide access to antiretroviral therapy (ART) has substantially improved the prognosis of patients living with HIV/AIDS (PLHIV). However, in resource-limited countries, sustaining ART programs to prevent drug resistance and treatment failure and to maximize the existing human resources is still challenging. In 2010, Vietnam had 254,000 PLHIV and 52,000 people accessed ART. Viral load (VL) testing has not been routinely performed for monitoring treatment failures due to the high cost and the necessity of advanced laboratory equipment. Peer support has been proven to improve quality of life, reduce stigma and to improve adherence to treatment. However, there is little known about the impact of peer adherence support on ART outcomes. The overall aim of this study was to assess the impact of peer support on virologic and immunologic treatment outcomes and mortality among HIV-infected patients by monitoring routinely a simple- and low- cost VL in a cluster randomized controlled trial in Quang Ninh, Vietnam. The primary outcome was virologic failure rate between intervention and control group. Methods: A total of 640 HIV-infected patients recruited from 59 clusters (communes) were randomized into either intervention or control group. Both groups received first-line ART regimens according to the National Treatment Guidelines and were followed up for 24 months. Viral load (ExaVirTM Load) and CD4 counts were measured every 6 months. Patients in the intervention group received enhanced adherence support by 14 peer supporters. Survival analyses with Kaplan-Meier curve and Cox proportional hazard model were used to identify survival rate and risk factors for deaths. Causes of death were assessed through medical records and verbal autopsy questionnaire. Cluster longitudinal and survival analyses with intention-to-treat were used to study time to virologic failure and CD4 trends and to compare between the intervention and control groups. At baseline, we monitored the spread of infection and prevalence of transmitted drug resistance mutations (TDRMs) by analyzing 63 1000bp pol-gene sequences generated from 63 treatment-naïve HIV-1 CRF01_AE patients. Through the cohort, we determined the feasibility, sensitivity and specificity of ExaVir Load in 605 HIV treatment-naïve patients and compared the correlation and agreement of 60 samples between Roche Cobas TaqMan® VL and ExaVir Load. Results: After 24 months of follow-up, 78% of the patients remained in the study, mortality rate was 11% (6.4/100 person-years), cumulative virologic failure rate (VL >1,000 copies/ml) was 7.2% and the median CD4 increase was 286 cells/μl. There were no significant differences between intervention and control groups in virologic failure rates (VL >1,000 copies/ml) [6.9% vs 7.5%, respectively, RR 0.93; (95%CI: 0.13-6.54), p=0.94], in the time to virologic failure [HR 1.0; (95%CI 0.5-1.7), p=0.94], in CD4 trends [Coeff. (95%CI: 0.2(-0.6;-0.9), p=0.69] and in mortality (Log-rank p=0.79). Risk factors for virologic failure were ART-non-naïve status [aHR 6.9;(95%CI 3.2-14.6); p100,000 copies/ml [aHR 2.3;(95%CI 1.2-4.3); p35 years, clinical stage 3 or 4, body mass index (BMI) 100,000 copies/ml. The TDRMs including Y181C, L210W, L74I and V75M were found in 4/63 patients (6.3%). Phylogenetic analysis for calculating the time of the most recent common ancestor (tMRCA) was shown in two distinct groups: the small group (n=3) had tMRCA in year 1997.5 and the larger group had tMRCA in 1989.8. The ExaVir Load and the Roche Cobas TaqMan showed a strong correlation (r2 =0.97), high agreement (log difference =0.34; 95% CI -0.35;1.03), high sensitivity (98%) and high specificity (100%). Conclusions: Enhanced adherence intervention by peer support had no impact on virologic failure and CD4 trends as well as on mortality after 24 months of ART initiation. Early deaths occurred among patients presented late to ART and majority of deaths were attributable to TB. Baseline VL >100,000 copies/ml was a predictive factor for virologic failure, CD4 changes and mortality. Transmitted drug resistance rate should be monitored regularly and prospectively in Vietnam. Using ExaVir Load is feasible to monitor efficacy of ART programs in resource-limited settings

The Determinants of Customer Satisfaction in Fast Food Industry

This primary objective of this study is to identify the determinants of customer satisfaction for KFC in Vietnam. Fast food industry developed rapidly in an emerging economy like Viet Nam. Current competition in Vietnamese fast food has required companies to pay more attention to customer satisfaction. Data is collected from KFC's customers in Ho Chi Minh City, Viet Nam. The research model is adopted from the SERVQUAL model (Parasuraman et al., 1988). The findings show that the main determinants of customer satisfaction of KFC Vietnam are Food Quality, Ambience, Price, Service Quality. Among these determinants, ambiance causes the most impact on customer satisfaction, following up by food quality and price. Managerial implications for KFC and other fast-food companies are discussed to improve customer satisfaction

Risk factors of dengue fever in an urban area in Vietnam: a case-control study

BackgroundDengue is a mosquito-borne flavivirus present in many metropolitan cities of tropical countries.MethodsDuring and after the dengue season (September 2018 to January 2019), we conducted a case-control study in order to determine the risk factors for dengue fever in Hanoi city, Vietnam. 98 dengue patients and 99 patients with other acute infections, such as Hepatitis B virus infection, were recruited at Department of Infectious Disease of Bach Mai national hospital in Hanoi. Patients were interviewed using a structured questionnaire covering demographic, housing, environmental factors and knowledge, attitude, and practice on dengue prevention and control. Univariate analysis and multivariable logistic regression were used to determine the risk factors of dengue status.ResultsThe mean score of knowledge items and practice items was only 7.9 out of total 19 points and 3.9 out of total 17 points, respectively. While the mean score of attitude items was 4.8 out of total 6 points. Multivariable logistic regression indicated that older patients had lesser risk of getting dengue infection as compared to younger adults aged 16-30, and patients living in peri-urban districts were less likely to suffer of dengue fever than patients living in central urban districts (OR=0.31; 95% CI 0.13-0.75). This study could not find any association with occupation, water storage habit, knowledge, attitude, or practice on dengue prevention.ConclusionsAll patients had a relatively low level of knowledge and practice on dengue prevention and control. However, the attitude of the participants was good. We found that age group and living district were the risk factors correlated with the dengue status. Communication programs on raising dengue awareness should be repeated all year round and target particular groups of adolescents, younger adults, landlords and migrants from other provinces to improve their knowledge and encourage them to implement preventive measures against dengue fever

Exploiting Idioms and Proverbs of Vietnamese Regions in Teaching Mathematics in Primary Schools

Mathematics and idioms, as well as proverbs, all reflect the laws of life. At the same time, primary school children may have heard idioms and proverbs before attending school. Therefore, there are many possibilities to exploit and apply idioms and proverbs in teaching mathematics in primary schools. This study aims to identify appropriate situations and apply idioms and proverbs in different regions of Vietnam to teaching mathematics. The researchers selected 1155 expressions related to mathematics from many typical pieces of research on idioms and proverbs in Vietnam. After surveying 1822 teachers three times in many provinces and cities in all 3 regions of Vietnam: the North, the Central and the South, the researchers have classified the data according to the criteria from closed to open-ended questions. The results show a prominent level of interest (level 4/5) of all teachers participating in the survey, and there is no difference in the effectiveness in the three regions, but there is a clear difference in regions in using idioms and proverbs. Particularly, identifying situations to teach geometric and quantitative knowledge, as well as probability and statistics, allows one to apply idioms and proverbs at a high level. It is concluded that if idioms and proverbs from Vietnamese regions are appropriately selected and applied in teaching mathematics in primary schools, they will contribute to improving students' mathematical ability and preserving the national cultural heritage. Doi: 10.28991/ESJ-2022-SIED-015 Full Text: PD

A modern purification by accelerated solvent extraction and centrifugal partition chromatography and biological evaluation of capsaicin from Capsicum chinense

A special alkaloid compound known as capsaicin, which can only be found in the fruit of the Capsicum plant, was isolated and tested for its anti-inflammatory activity. The purpose of this work is to establish a simple and quick approach for capsaicin purification utilizing centrifugal partition chromatography (CPC) as well as an effective method - accelerated solvent extraction (ASE), for extracting capsaicin from Capsicum chinense. After purification, capsaicin was validated by HPLC-DAD at 281 nm to be > 90% purity. The in vivo anti-inflammatory activity of the isolated capsaicin was also investigated, and the IC50 value of the capsaicin was determined to be 57.61 µg/mL. The current work emphasizes how an ASE and CPC system may combine to extract high-purity capsaicin from Capsicum chinense, which have the anti-inflammatory activity, as we evaluated in the experiment

Spontaneous Symmetry Breaking of Solitons Trapped in a Double-Gauss Potentials

We consider an extended model of the model considered before with double-square potential, namely one-dimensional (1D) nonlinear Schrödinger equation (NLSE) with self-focusing nonlinearity and a 1D double-gauss potential. Spontaneous symmetry breaking has been presented in terms of the control parameter which is propagation constant in the case of optics and chemical potential in the of Bose-Einstein Condensate (BEC), correspondingly. The numerical simulations predict a bifurcation breaking the symmetry of 1D trapped in the double-gauss potential of the supercritical type as in the case of double-square potential. Furthermore we have constructed bifurcation diagrams considering the stability of solitons with three methods: the method using Vakhitov–Kolokolov (V-K) Stability Criterion, Pseudospectral Method and Method for Linear-Stability Eigenvalues. It will be shown that for our model the results obtained are the same for these three methods but the third one is the fastest

Meningitis patients with Angiostrongylus cantonensis may present without eosinophilia in the cerebrospinal fluid in northern Vietnam.

BACKGROUND: Eosinophilic meningitis (EM) is a rare clinical syndrome caused by both infectious and noninfectious diseases. In tropical pacific countries, Angiostrongylus cantonensis is the most common cause. However, the EM definition varies in the literature, and its relation to parasitic meningitis (PM) remains unclear. METHODOLOGY/PRINCIPAL FINDINGS: Adult and adolescent patients of 13 years old or above with suspected central nervous system (CNS) infections with abnormal CSF findings were prospectively enrolled at a tertiary referral hospital in Hanoi, Vietnam from June 2012 to May 2014. Patients with EM or suspected PM (EM/PM) were defined by the presence of either ≥10% eosinophils or an absolute eosinophil cell counts of ≥10/mm3 in the CSF or blood eosinophilia (>16% of WBCs) without CSF eosinophils. In total 679 patients were enrolled: 7 (1.03%) had ≥10% CSF eosinophilia, 20 (2.95%) had ≥10/mm3 CSF eosinophilia, and 7 (1.03%) had >16% blood eosinophilia. The patients with ≥10% CSF eosinophilia were significantly younger (p = 0.017), had a lower body temperature (p = 0.036) than patients with ≥10/mm3 CSF eosinophilia among whom bacterial pathogens were detected in 72.2% (13/18) of those who were tested by culture and/or PCR. In contrast, the characteristics of the patients with >16% blood eosinophilia resembled those of patients with ≥10% CSF eosinophilia. We further conducted serological tests and real-time PCR to identify A. cantonensis. Serology or real-time PCR was positive in 3 (42.8%) patients with ≥10% CSF eosinophilia and 6 (85.7%) patients with >16% blood eosinophilia without CSF eosinophils but none of patients with ≥10/mm3 CSF eosinophilia. CONCLUSIONS: The etiology of PM in northern Vietnam is A. cantonensis. The eosinophil percentage is a more reliable predictor of parasitic EM than absolute eosinophil count in the CSF. Patients with PM may present with a high percentage of eosinophils in the peripheral blood but not in the CSF

Incidence of AIDS-Defining Opportunistic Infections and Mortality during Antiretroviral Therapy in a Cohort of Adult HIV-Infected Individuals in Hanoi, 2007-2014

Background: Although the prognosis for HIV-infected individuals has improved after antiretroviral therapy (ART) scale-up, limited data exist on the incidence of AIDS-defining opportunistic infections (ADIs) and mortality during ART in resource-limited settings. Methods: HIV-infected adults in two large hospitals in urban Hanoi were enrolled to the prospective cohort, from October 2007 through December 2013. Those who started ART less than one year before enrollment were assigned to the survival analysis. Data on ART history and ADIs were collected retrospectively at enrollment and followed-up prospectively until April 2014. Results: Of 2,070 cohort participants, 1,197 were eligible for analysis and provided 3,446 person-years (PYs) of being on ART. Overall, 161 ADIs episodes were noted at a median of 3.20 months after ART initiation (range 0.03–75.8) with an incidence 46.7/1,000 PYs (95% confidence interval [CI] 39.8–54.5). The most common ADI was tuberculosis with an incidence of 29.9/1,000 PYs. Mortality after ART initiation was 8.68/1,000 PYs and 45% (19/45) died of AIDS-related illnesses. Age over 50 years at ART initiation was significantly associated with shorter survival after controlling for baseline CD4 count, but neither having injection drug use (IDU) history nor previous ADIs were associated with poor survival. Semi-competing risks analysis in 951 patients without ADIs history prior to ART showed those who developed ADIs after starting ART were at higher risk of death in the first six months than after six months. Conclusion: ADIs were not rare in spite of being on effective ART. Age over 50 years, but not IDU history, was associated with shorter survival in the cohort. This study provides in-depth data on the prognosis of patients on ART in Vietnam during the first decade of ART scale-up

Challenges in diagnosing scrub typhus among hospitalized patients with undifferentiated fever at a national tertiary hospital in northern Vietnam

BACKGROUND: Scrub typhus (ST) is a leading cause of non-malarial febrile illness in Southeast Asia, but evidence of its true disease burden is limited because of difficulties of making the clinical diagnosis and lack of adequate diagnostic tests. To describe the epidemiology and clinical characteristics of ST, we conducted an observational study using multiple diagnostic assays at a national tertiary hospital in Hanoi, Vietnam. METHODOLOGY/PRINCIPAL FINDINGS: We enrolled 1,127 patients hospitalized with documented fever between June 2012 and May 2013. Overall, 33 (2.9%) patients were diagnosed with ST by PCR and/or screening of ELISA for immunoglobulin M (IgM) with confirmatory tests: 14 (42.4%) were confirmed by indirect immunoperoxidase assay (IIP), and 19 (57.6%) were by IIP and PCR. Living by farming, conjunctival injection, eschar, aspartate aminotransferase elevation, and alanine aminotransferase elevation were significantly associated with ST cases (adjusted odds ratios (aORs): 2.8, 3.07, 48.8, 3.51, and 4.13, respectively), and having a comorbidity and neutrophilia were significantly less common in ST cases (aORs: 0.29 and 0.27, respectively). The majority of the ST cases were not clinically diagnosed with rickettsiosis (72.7%). Dominant IIP reactions against a single antigen were identified in 15 ST cases, whereas indistinguishably high reactions against multiple antigens were seen in 11 ST cases. The most frequently observed dominant IIP reaction was against Karp antigen (eight cases) followed by Gilliam (four cases). The highest diagnostic accuracy of IgM ELISA in acute samples was 78%. In a phylogenetic analysis of the 56-kDa type-specific antigen gene, the majority (14 cases) were located in the Karp-related branch followed by the Gilliam-related (two cases), Kato-related (two cases), and TA763-related clades (one case). CONCLUSIONS/SIGNIFICANCE: Both the clinical and laboratory diagnoses of ST remain challenging at a tertiary hospital. Implementation of both serological and nucleic acid amplification assays covering endemic O. tsutsugamushi strains is essential