Clinical and laboratory substantiation of complex for prevention and treatment periodontal diseases in women with polycystic ovary syndrome

Воспалительные и воспалительно-деструктивные заболевания тканей пародонта (хронический гингивит и пародонтит) являются одной из наиболее важных медицинских и социально-экономических проблем современного общества. Это обусловлено как массовостью распространения данной патологии (с тенденцией к дальнейшему повышению показателей), что является одной из основных причин полной потери зубов, так и негативным влиянием очагов хронической инфекции в пародонтальном комплексе на состояние соматического здоровья. В соответствии с данными ВОЗ около 95 % взрослого и 80 % детского населения планеты имеют те или иные признаки заболеваний пародонта. Высокий уровень заболеваемости отмечается в возрасте 20–44 года (65–95 %) и 15–19 лет (55–89 %), при этом женщины болеют чаще, чем мужчины. Цель исследования: клинико-лабораторное обоснование комплекса для профилактики и лечения заболеваний тканей пародонта у женщин с синдромом поликистозных яичников на основании оценки пародонтального, гормонального статуса и биохимических маркеров гомеостаза полости рта.Сlinical and laboratory substantiation of complex for prevention and treatment periodontal diseases in women with polycystic ovary syndrome (PCOS) based on the assessment of periodontal status, hormonal status and changes of biochemical markers of homeostasis in oral cavity

Влияние комбинированного применения антиоксидантов при патологии пародонта у женщин с синдромом поликистоза яичников

Мета роботи – встановлення особливостей змін біохімічних маркерів у жінок із генералізованим пародонтитом середнього ступеня тяжкості на тлі синдрому полікістозу яєчників при використанні оптимальної стратегії профілактики та лікування пародонтиту. Обстежили в динаміці 2 групи пацієнтів по 25 жінок із генералізованим пародонтитом середнього ступеня тяжкості на тлі синдрому полікістозу яєчників. Контрольна група – 25 жінок. У першій групі застосовували лікувально-профілактичний комплекс (місцево – еліксир «Біодент-4» та мукозальний гель «ЕКСО», внутрішньо – препарат «Амарант Королівський» на основі олії амаранту та таблетки «ЕКСО»), у другій – традиційне лiкування.The purpose of this work was to assess the features of biochemical markers changes in women with periodontal pathology associated with polycystic ovarian syndrome using optimal management of chronic generalized periodontitis prevention and treatment. There were examined two groups of 25 women each in the course of moderate severity chronic generalized periodontitis associated with polycystic ovarian syndrome. The control group consisted of 25 women. The treatment-and-prophylactic complex (locally – mouthwash “Biodent-4” and mucosal gel “EKSO”, orally – an amaranth oil-based drug and tablets “EKSO”) was used in the frst group; only conventional treatment was used in the second group.Цель работы – определение особенностей изменений биохимических маркеров у женщин с патологией пародонта на фоне синдрома поликистоза яичников при использовании оптимальной стратегии профилактики и лечения хронического генерализованного пародонтита. В динамике обследованы 2 группы пациентов по 25 женщин с хроническим генерализованным пародонтитом средней степени тяжести на фоне синдрома поликистоза яичников. Контрольная группа составляла 25 женщин. В первой группе применяли лечебно-профилактический комплекс (местно – эликсир «Биодент-4» и мукозальный гель «ЭКСО», внутрь – препарат на основе масла амаранта «Амарант Королевский» и таблетки «ЭКСО»), во второй – традиционное лечение

ジペプチジルペプチターゼ4阻害剤（シタグリプチン）およびアンギオテンシンⅡ1型受容体遮断薬（ロサルタン）の併用療法は非糖尿病ラットモデルにおける非アルコール性脂肪肝炎の進行を抑制する

AIM: Dipeptidyl peptidase-4 (DPP4) inhibitors (DPP4-I) are oral glucose-lowering drugs for type 2 diabetes mellitus. Previously, we reported that DPP4-I (sitagliptin) exerted suppressive effects on experimental liver fibrosis in rats. Blockade of the renin-angiotensin system by angiotensin-II type 1 receptor blocker (losartan), commonly used in the management of hypertension, has been shown to significantly alleviate hepatic fibrogenesis and carcinogenesis. We aimed to elucidate the effects and possible mechanisms of a sitagliptin + losartan combination on the progression of non-diabetic non-alcoholic steatohepatitis (NASH) in a rat model. METHODS: To induce NASH, Fischer 344 rats were fed a choline-deficient L-amino acid-defined diet for 12 weeks. We elucidated the chemopreventive effects of sitagliptin + losartan, especially in conjunction with hepatic stellate cell (HSC) activation, angiogenesis, and oxidative stress, all known to play important roles in the progression of NASH. RESULTS: Sitagliptin + losartan suppressed choline-deficient L-amino acid-defined diet-induced hepatic fibrogenesis and carcinogenesis. The combination treatment exerted a greater inhibitory effect than monotherapy. These inhibitory effects occurred almost concurrently with the suppression of HSC activation, neovascularization, and oxidative stress. In vitro studies showed that sitagliptin + losartan inhibited angiotensin II-induced proliferation and expression of transforming growth factor-β1 and α1 (I)-procollagen mRNA of activated HSC and in vitro angiogenesis, in parallel with the suppression observed in in vivo studies. CONCLUSIONS: The widely and safely used sitagliptin + losartan combination treatment in clinical practice could be an effective strategy against NASH.博士（医学）・乙第1406号・平成29年9月27日© 2016 The Japan Society of HepatologyCopyright © 2016 John Wiley & Sons, Inc. All Rights Reserved.This is the pre-peer reviewed version of the following article: Hepatology research [Epub ahead of print] (2016 Dec 28), which has been published in final form at http://dx.doi.org/10.1111/hepr.12860. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving

Localization of the murine cholecystokinin A and B receptor genes

We have determined the chromosomal locations of the two cholecystokinin (CCK) receptor genes in the mouse. Genetic localization utilized an interspecific backcross panel formed from the cross (C57BL/6J x Mus spretus ) F 1 x Mus spretus . Genomic DNAs from 94 individuals in the backcross were analyzed by Southern hybridization with rat CCK A and CCK B receptor cDNA probes. Unique map positions were determined by haplotype analysis with 650 previously mapped loci in the mouse backcross. The CCK A receptor gene ( Cckar ) mapped to mouse Chromosome (Chr) 5, in tight linkage with the DNA marker D5Bir8 . The CCK B receptor gene ( Cckbr ) mapped to mouse Chr 7, tightly linked to the β-hemoglobin locus ( Hbb ). This localization places Cckbr in the same region as the mouse obesity mutation tubby ( tub ), which also maps near Hbb (2.4±1.4 cM). Since CCK can function as a satiety factor when administered to rodents, localization of Cckbr near the tub mutation identifies this receptor as a possible candidate gene for this obesity mutation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47021/1/335_2004_Article_BF00352408.pd

THE PECULIARITIES OF ORAL CAVITY ENZYME FUNCTIONING IN WOMEN WITH POLYCYSTIC OVARIAN SYNDROME

The aim of the study was to evaluate the functioning of the enzyme systems of oral cavity in women with polycystic ovarian syndrome. There was found that every fourth patient with Polycystic ovarian syndrome (PCOS) has manifestations of chronic catarrhal gingivitis. The level of the oral hygiene in both groups was comparable studies: 1,120,2 and 0,940,1 points accounting respectively. PMA index in women with PCOS was 35,3 ± 2,3%, while in the second group it was 11,1 ± 1,2%. Accordingly, the index CPITN in the patients of group was 2,90,2 points, while the second group of patients had 2,10,2 points (p <0,05). The patients with PCOS were marked and increased in the concentration of MDA to 0,130,02 mmol / L, the reduction in alkaline phosphatase activity was to 0,060,01 mkkat / L, the increasing activity of urease was to 0,080,01 and the decreasing production of lysozyme was to 113,013,6 U / l. It is established that the content of MDA correlates with the activity of lysozyme (r = 0,67), alkaline phosphatase (r = 0,67) and catalase (r = 0,73). The problems of the pathogenesis of periodontal lesions in women with polycystic ovary syndrome were discussed

The influence of combined antioxidants usage on periodontal pathology in women with polycystic ovarian syndrome

The purpose of this work was to assess the features of biochemical markers changes in women with periodontal pathology associated with polycystic ovarian syndrome using optimal management of chronic generalized periodontitis prevention and treatment. Materials and methods. There were examined two groups of 25 women each in the course of moderate severity chronic generalized periodontitis associated with polycystic ovarian syndrome. The control group consisted of 25 women. The treatment-and-prophylactic complex (locally – mouthwash “Biodent-4” and mucosal gel “EKSO”, orally – an amaranth oil-based drug and tablets “EKSO”) was used in the first group; only conventional treatment was used in the second group. Results. In the first group urease activity decreased by 36.0 % after a month of treatment, in 6 months - by 46.0 %, in 12 months - by 62.4 %; lysozyme increased within 1 month by 40.5 %, in 6 months – by 52.4 %, in 12 months – by 53.6 %, diene conjugates in 1 month – by 32.0 %, in 6 months – by 43.5 %, in 12 months – by 42,8 %; malondialdehyde in 1 month by 31.7 %, in 6 months – by 43.9 %, in 12 months – by 42.6 %. Catalase increased in 1 month by 40.0 %, in 6 months – by 52.0 %, in 12 months – by 62.0 %. In the second group urease activity decreased by 12.6 % in 1 month, in 6 months – by 25.3 %, in 1 year – by 34.2 %. The level of diene conjugates decreased in 6 months by 22.3 %, in 12 months – by 24.8 %. Activity of superoxide dismutase decreased in 1 month by 20.0 %, in 6 months – by 22.6 %, in 12 months – by 27.8 %, glutathione peroxidase in 6 months – by 28.9 %, in 12 – by 26.3 %, catalase and lysozyme increased in 12 months by 36.7 and 30.5 %, respectively. Conclusions. The oral fluid biochemical parameters dynamics in women with moderate severity chronic generalized periodontitis associated with polycystic ovarian syndrome evidences the high efficiency of the prophylactic and therapeutic complex. Positive changes in the periodontal status of patients can be explained by a decrease in the intensity of lipid peroxidation, an inflammatory response, and activation of antioxidant protection in the oral cavity