Race affects SVR12 in a large and ethnically diverse hepatitis C-infected patient population following treatment with direct-acting antivirals: Analysis of a single-center Department of Veterans Affairs cohort.

Hepatitis C virus (HCV) infection is a major cause of chronic liver disease. HCV cure has been linked to improved patient outcomes. In the era of direct-acting antivirals (DAAs), HCV cure has become the goal, as defined by sustained virological response 12 weeks (SVR12) after completion of therapy. Historically, African-Americans have had lower SVR12 rates compared to White people in the interferon era, which had been attributed to the high prevalence of non-CC interleukin 28B (IL28B) type. Less is known about the association between race/ethnicity and SVR12 in DAA-treated era. The aim of the study is to evaluate the predictors of SVR12 in a diverse, single-center Veterans Affairs population. We conducted a retrospective study of patients undergoing HCV therapy with DAAs from 2014 to 2016 at the VA Greater Los Angeles Healthcare System. We performed a multivariable logistic regression analysis to determine predictors of SVR12, adjusting for age, HCV genotype, DAA regimen and duration, human immunodeficiency virus (HIV) status, fibrosis, nonalcoholic fatty liver disease (NAFLD) fibrosis score, homelessness, mental health, and adherence. Our cohort included 1068 patients, out of which 401 (37.5%) were White people and 400 (37.5%) were African-American. Genotype 1 was the most common genotype (83.9%, N = 896). In the adjusted models, race/ethnicity and the presence of fibrosis were statistically significant predictors of non-SVR. African-Americans had 57% lower odds for reaching SVR12 (adj.OR = 0.43, 95% CI = 1.5-4.1) compared to White people. Advanced fibrosis (adj.OR = 0.40, 95% CI = 0.26-0.68) was also a significant predictor of non-SVR. In a single-center VA population on DAAs, African-Americans were less likely than White people to reach SVR12 when adjusting for covariates

Membrane-bound β-catenin degradation is enhanced by ETS2-mediated Siah1 induction in Helicobacter pylori-infected gastric cancer cells.

β-catenin has two different cellular functions: intercellular adhesion and transcriptional activity. The E3 ubiquitin ligase Siah1 causes ubiquitin-mediated degradation of the cytosolic β-catenin and therefore, impairs nuclear translocation and oncogenic function of β-catenin. However, the effect of Siah1 on the cell membrane bound β-catenin has not been studied. In this study, we identified that the carcinogenic bacterium H. pylori increased ETS2 transcription factor-mediated Siah1 protein expression in gastric cancer cells (GCCs) MKN45, AGS and Kato III. Siah1 protein level was also noticeably higher in gastric adenocarcinoma biopsy samples as compared to non-cancerous gastric epithelia. Siah1 knockdown significantly decreased invasiveness and migration of H. pylori-infected GCCs. Although, Siah1 could not increase degradation of the cytosolic β-catenin and its nuclear translocation, it enhanced degradation of the membrane-bound β-catenin in the infected GCCs. This loss of membrane-bound pool of β-catenin was not associated with the proteasomal degradation of E-cadherin. Thus, this work delineated the role of Siah1 in increasing invasiveness of H. pylori-infected GCCs

Pulmonary Echinococcal Cyst with a Filamentous Fungus Co-Infection

Fungal infections are known to colonize the preexisting lung cavities formed as a result of diseases like tuberculosis, sarcoidosis, bronchiectasis and cavitatary neoplasia, mostly encountered in immunocompromised patients. Pulmonary echinococcal cysts have been reported coexistent with cryptococcosis and other saprophytic mycosis, but the coexistence of aspergillosis and echinococcal cyst is extremely rare and occasionally been reported in English literature. Active invasion and proliferation of the fungi in the laminated ectocyst of the echinococcal cyst is very unusual. We report a case of 60 years old immunocompetent female, presented with cough, chest pain and shortness of breath. The chest X- ray showed a large thick walled cavity in the lower and mid zone of right lung with positive water lily sign. Surgical enucleation of the echinococcal cyst revealed aspergilloma involving the cavity with massive invasion of laminated ectocyst by filamentous fungus, morphologically resembling an Aspergillus species and was further treated with Itraconazole for 3 months. This unique coexistence of active pulmonary echinococcosis and aspergillosis is being reported because of its rarity and clinical importance for its management.Keywords: Aspergillosis, echinococcosis, echinococcal cyst, pulmonary, mycosis

Coordination properties of vic-isonitrosoimines in their copper (II) and palladium (II) complexes

Preparation and structural characterization of palladium (II) complexes of ligands III-V and copper (II) complexes of III are reported. The elemental analyses of the complexes show that the metal: ligand ratio is 1:2. The electrical conductance in acetone shows the non-electrolytic nature of the complexes. The diamagnetic character suggests a gross square-planar geometry for the palladium (II) complexes. Copper (II) complexes are paramagnetic with μeff.~1·90 B.M. Spectral data suggest that in all the complexes the ligand coordinates to the metal (II) symmetrically through isonitroso-nitrogen and imine-nitrogen, forming a five membered chelate ring. Amine-exchange reactions of the complexes are discussed and compared on the basis of their structures

Magnetoelectric effect due to local noncentrosymmetry

Magnetoelectrics often possess ions located in noncentrosymmetric surroundings. Based on this fact we suggest a microscopic model of magnetoelectric interaction and show that the spin-orbit coupling leads to spin-dependent electric dipole moments of the electron orbitals of these ions, which results in non-vanishing polarization for certain spin configurations. The approach accounts for the macroscopic symmetry of the unit cell and is valid both for commensurate and complex incommensurate magnetic structures. The model is illustrated by the examples of MnWO4, MnPS3 and LiNiPO4. Application to other magnetoelectrics is discussed.Comment: 11 pages, 2 figures, 2 table

Bioenergy crop production and carbon sequestration potential under changing climate and land use: A case study in the upper River Taw catchment in southwest England

Reductions in CO2 emissions are essential to support the UK in achieving its net zero policy objective by around mid-century. Both changing climate and land use change (LUC) offer an opportunity to deploy suitable bioenergy crops strategically to enhance energy production and C sequestration to help deliver net zero through capturing atmospheric CO2. Against this background, we applied process-based models to evaluate the extent of net primary productivity (NPP) losses/gains associated with perennial bioenergy crops and to assess their C sequestration potential under changing climate in the upper River Taw observatory catchment in southwest England. In so doing, we also determined whether LUC from permanent grassland to perennial bioenergy crops, considered in this study, can increase the production and C sequestration potential in the study area. The results show that a warming climate positively impacts the production of all crops considered (permanent grassland, Miscanthus and two cultivars of short rotation coppice (SRC) willow). Overall, Miscanthus provides higher aboveground biomass for energy compared to willow and grassland whereas the broadleaf willow cultivar ‘Endurance’ is best suited, among all crops considered, for C sequestration in this environment, and more so in the changing climate. In warmer lowlands, LUC from permanent grassland to Miscanthus and in cooler uplands from permanent grassland to ‘Endurance’, enhances NPP. Colder areas are predicted to benefit more from changing climate in terms of above and belowground biomass for both Miscanthus and willow. The study shows that above LUC can help augment non-fossil energy production and increase C sequestration potential if C losses from land conversion do not exceed the benefits from LUC. In the wake of a changing climate, aboveground biomass for bioenergy and belowground biomass to enhance carbon sequestration can be managed by the careful selection of bioenergy crops and targeted deployment within certain climatic zones

Farm-scale zoning of extreme temperatures in Southern Mallee, Victoria, Australia

Extreme temperatures around the flowering of wheat have the potential to reduce grain yield and at farm scale their impact can be spatially variable. In this study, the zoning of extreme temperatures, using data collected over two years, was carried out for a 164 ha farm in the Southern Mallee, Victoria, Australia in order to identify areas at high risk of extreme temperatures around the time of the flowering of wheat. Twenty-five data loggers were installed at 0.8 m height across the farm to spatially record the daily course of temperatures around the average date of flowering for the region. After applying the zoning algorithms, the maps of different temperature zones were produced by spatial interpolation in ArcView 3.2. It was found that in 2003, about 58% of the farm area was prone to exposure to higher temperatures and about 73% to the lower temperatures whereas in 2004 about 46% of the farm area was prone to exposure to higher temperatures and about 39% to the lower temperatures

Modification of a spatially referenced crop model to simulate the effect of spatial pattern of subsoil salinity

High levels of subsoil salinity limit the growth and yield of dryland cereals in the Victorian southern Mallee, Australia. Currently available crop simulation models of wheat production perform poorly in this region, presumably due to their inability to account for subsoil limitations, mainly salinity. The objective of this work was to modify a spatially referenced Water and Nitrogen Management Model (WNMM) to account for the spatial pattern of subsoil salinity, by adjusting crop water uptake, in order to explain the spatial variation in wheat yield in this area. Measurements of above-ground biomass and yield of wheat, and the profile of soil salinity (0–80 cm) were made at 40 locations across an 88 ha paddock (35.78°S, 142.98°E) in the Victorian southern Mallee. The S-shaped water stress response function for crop water uptake proposed by van Genuchten (1987) was explored to modify the WNMM by adjusting the water uptake due to salinity, which significantly improved yield simulation over the original WNMM. The improvement in the model's ability to simulate wheat yield indicates that the subsoil salinity limits crop performance in the area. The incorporation of a salinity function in spatial crop models offers potential for simulating yield across a landscape and thus practicing precision agriculture provided salinity impact is considered dynamically

Effect of topography on farm-scale spatial variation in extreme temperatures in the Southern Mallee of Victoria, Australia

Extreme temperatures around flowering of wheat have the potential to reduce grain yield and at farm scale their impact can be spatially variable depending on topography. Twenty-five data loggers were installed at 0.8-m height across a 164-ha farm in the southern Mallee of Victoria, Australia to spatially record the daily course of temperatures around the average date of flowering of wheat in the region. The experiment was conducted during 2- years period. In 1 year, the farm had no crop cover and in another year the farm had a wheat crop. Multiple linear regression analysis techniques were used to fit models relating daily extreme temperatures to the farm topographic features of elevation, aspect and slope, and the average maximum and minimum temperatures of each day at the farm in order to identify areas of high risk of extreme temperatures around the time of the flowering of wheat. The fitted regression models explained 90% and 97% of the variability in maximum and minimum temperatures, respectively, when the farm had no crop cover and 80% and 94% of the variability in maximum and minimum temperatures, respectively, when the farm had a wheat crop cover. When the farm had no crop, only minimum temperature was partially explained by the topography however, both maximum and minimum temperatures were partially explained by the topography when the farm had a wheatcrop. From this study it was concluded that, (1) high temperature variations were found across the farm (2) temperature variations were only partially explained from the developed model presumably due to the flatter topography of the farm and (3) the relationships obtained from this study could be used in a crop model which can explain variation in grain yield based on the topography of a field

Impact of spatially variable soil salinity on crop physiological properties, soil water content and yield of wheat in a semi arid environment

In the Birchip region of the Victorian southern Mallee, Australia, subsoil salinity is an important factor determining crop growth and yield. Crop simulation models have performed poorly in this region, presumably due to their inability to account for subsoil constraints, mainly salinity. The objective of this work was to study the impact of subsoil salinity on crop physiological properties, growth, water use and yield of a wheat crop. From a calibrated electromagnetic survey (EM 38) over an area 7 m wide by 100 m long, three sites of low, medium and high salinity levels were identified. For each site, soil electrical conductivity was measured and the values averaged for the depth 0-70 cm were 0.25, 1.14 and 1.63 dS/m at the sites with low, medium and high salinity, respectively. Further, at different stages of crop growth, radiation interception by the canopy as well as soil water content were measured while plant samples were collected to estimate crop physiological properties. Grain yield at each salinity site was also measured. All the physiological properties and yield were negatively affected by increasing salinity levels due to less water use and radiation interception. Compared to the low salinity level, medium and high salinity levels reduced the above-ground dry weight of the crop at harvest by 40% and 41%, accumulated intercepted radiation by 23% and 37%, radiation use efficiency by 25% and 52%, water use by 18% and 35% and grain yield by 41% and 48%, respectively